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The map label for this gene is dusC [H]

Identifier: 147674229

GI number: 147674229

Start: 665940

End: 666413

Strand: Reverse

Name: dusC [H]

Synonym: VC0395_A0623

Alternate gene names: 147674229

Gene position: 666413-665940 (Counterclockwise)

Preceding gene: 147674360

Following gene: 147674652

Centisome position: 22.04

GC content: 51.27

Gene sequence:

>474_bases
GTGCACGCGCGCACCAAAGCCGGAGGCTACAAGGCCAGCGAAATCAAATGGCATTACATTGACCAGATCCGCCAAAAATG
CAGTATTCCATTGATTGCTAACGGTGAAGTGTGGAATTACGCCGATGGTCAAGCTTGCATCCAAACCACAGGTGTCGATT
CACTCATGGTGTGCCGCGGCGCGCTCAACGTGCCGAATTTAGGCAATGTGGTCAAACACAATCACGCCGCCATGCCTTGG
CATGAAGTGGTGGATTTGTTGCTGCGCTACACGCAATTTGAAGTCCGTGGTGACAAAGGCAAATACTACCCAAACCGTAT
TAAGCAGTGGTTTGCTTATCTGCGCCACGCCTACCCTCAGGCTAATGAGCTGTTTGGTGAGTTACGTACGTTGACTCAAG
TCGAGCCGATCGTTGATCAGTTACATCGCTATCGCGATCAACTCCATCATGCTGCGGCCGAGCTACCTGTCTAA

Upstream 100 bases:

>100_bases
CATTCCTGTTTCGGCGAAAATCCGCTTAGGTTGGGAAGATCCGGAAGATTGTTTTGAGATTGTCGATGCCGTTGCGTAAG
CTAAAGCCGATGAGCTCACT

Downstream 100 bases:

>100_bases
TGAGTAAAACAACGAATCAGTAAATCAACTGATCAGTAAACCAACTGATTTTTGCCGCTGGTTTTCGCTACATACAACTT
TTCATCAGCGGCTTTAAACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHNHAAMPW
HEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAELPV

Sequences:

>Translated_157_residues
MHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHNHAAMPW
HEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAELPV
>Mature_157_residues
MHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRGALNVPNLGNVVKHNHAAMPW
HEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAELPV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=131, Percent_Identity=51.9083969465649, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 18094; Mature: 18094

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCEECCCCCHHHHHHCCCHHHHHHHC
ALNVPNLGNVVKHNHAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQ
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCC
ANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAELPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHARTKAGGYKASEIKWHYIDQIRQKCSIPLIANGEVWNYADGQACIQTTGVDSLMVCRG
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCEECCCCCHHHHHHCCCHHHHHHHC
ALNVPNLGNVVKHNHAAMPWHEVVDLLLRYTQFEVRGDKGKYYPNRIKQWFAYLRHAYPQ
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCC
ANELFGELRTLTQVEPIVDQLHRYRDQLHHAAAELPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]