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The map label for this gene is truC

Identifier: 147674217

GI number: 147674217

Start: 437431

End: 438165

Strand: Direct

Name: truC

Synonym: VC0395_A0411

Alternate gene names: 147674217

Gene position: 437431-438165 (Clockwise)

Preceding gene: 147674977

Following gene: 147674601

Centisome position: 14.46

GC content: 50.48

Gene sequence:

>735_bases
ATGCTCGAGATTGTTTATCAAGATGAATATTTGGTGGCGGTCAATAAACCGGCTGGCATGTTGGTGCACCGTTCATGGCT
CGACAAGCATGAAACCGAGTTTGTGATGCAGACCCTGCGCGATCAAATTGGTCAGCACGTTTTCCCGCTACACCGTCTGG
ATCGCCCGACATCCGGTGTGTTGGTGTTTGCGCTTTCGAGTCAGATTGCCTCTGAAGTGATGCCGATGTTTGCCAATCAT
GAGATGGAGAAAACCTACCACGCCATTGTGCGCGGCTGGATTGAAGAGGCAAATGTGCTCGATTATCCACTCAAAGAAGA
GCTCGACAAAATTGCCGATAAATTTGCCAAGCAAGATAAAGAAGCGCAGTCGGCGGTGACAGCTTATCGGCCACTCGCCA
AAGTGGAACTGCCCATTGCTACGGGAAAGTTTGCCACTACCCGCTATTGCTTGATGGAGATGCAACCGAAGACCGGGCGC
AAACATCAGCTGCGTCGTCATATGGCACATCTGCGTCATCCGATAGTGGGTGATACCACCCATGGCGATGGAGTTCATAA
CCGACTGTTTCGTGAACACTTTTCCGCGCAACGTTTGATGCTGCATGCCAGCGAGTTACGTTTTATGCATCCTTATACCC
AGCAGCCGTTAGTGATTCGCGCTGGACTAGATGAAGTGTGGCAAGGCTTGCTTAGTGAGTTTGGTTGGCAAGAGTCGCTG
TTAATCAACGCTTGA

Upstream 100 bases:

>100_bases
TTTCGCGCTGGAGCCGTATTTCTCAGAAGTGTGGCAAGGTGAGGCCCGTTATCAAGCGGTGCTTGCGGTCATTCAACAAA
TCGACAAGGTGTGCCAATAG

Downstream 100 bases:

>100_bases
TGATTTTCTCAATCAGCCCAATAAAAAAGCCTTCATCATGAAGGCTTTTCTCTTTCTGATTTCTACCTCAGCGACGTTAA
GCGGCGGGTGGAAAGGACTA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLEIVYQDEYLVAVNKPAGMLVHRSWLDKHETEFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSQIASEVMPMFANH
EMEKTYHAIVRGWIEEANVLDYPLKEELDKIADKFAKQDKEAQSAVTAYRPLAKVELPIATGKFATTRYCLMEMQPKTGR
KHQLRRHMAHLRHPIVGDTTHGDGVHNRLFREHFSAQRLMLHASELRFMHPYTQQPLVIRAGLDEVWQGLLSEFGWQESL
LINA

Sequences:

>Translated_244_residues
MLEIVYQDEYLVAVNKPAGMLVHRSWLDKHETEFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSQIASEVMPMFANH
EMEKTYHAIVRGWIEEANVLDYPLKEELDKIADKFAKQDKEAQSAVTAYRPLAKVELPIATGKFATTRYCLMEMQPKTGR
KHQLRRHMAHLRHPIVGDTTHGDGVHNRLFREHFSAQRLMLHASELRFMHPYTQQPLVIRAGLDEVWQGLLSEFGWQESL
LINA
>Mature_244_residues
MLEIVYQDEYLVAVNKPAGMLVHRSWLDKHETEFVMQTLRDQIGQHVFPLHRLDRPTSGVLVFALSSQIASEVMPMFANH
EMEKTYHAIVRGWIEEANVLDYPLKEELDKIADKFAKQDKEAQSAVTAYRPLAKVELPIATGKFATTRYCLMEMQPKTGR
KHQLRRHMAHLRHPIVGDTTHGDGVHNRLFREHFSAQRLMLHASELRFMHPYTQQPLVIRAGLDEVWQGLLSEFGWQESL
LINA

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI221316667, Length=215, Percent_Identity=34.4186046511628, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI221316663, Length=233, Percent_Identity=31.3304721030043, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI17158025, Length=227, Percent_Identity=28.6343612334802, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI23308689, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789155, Length=243, Percent_Identity=65.4320987654321, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=31.3304721030043, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786244, Length=234, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788946, Length=237, Percent_Identity=27.4261603375527, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=212, Percent_Identity=28.7735849056604, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320168, Length=185, Percent_Identity=28.1081081081081, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324506, Length=185, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24583449, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583447, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583445, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921090, Length=179, Percent_Identity=28.4916201117318, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUC_VIBCH (Q9KTL4)

Other databases:

- EMBL:   AE003852
- PIR:   G82266
- RefSeq:   NP_230535.1
- ProteinModelPortal:   Q9KTL4
- SMR:   Q9KTL4
- GeneID:   2614117
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0888
- TIGR:   VC_0888
- HOGENOM:   HBG750656
- OMA:   GCDRLML
- ProtClustDB:   PRK11112
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 28232; Mature: 28232

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIVYQDEYLVAVNKPAGMLVHRSWLDKHETEFVMQTLRDQIGQHVFPLHRLDRPTSGV
CEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCE
LVFALSSQIASEVMPMFANHEMEKTYHAIVRGWIEEANVLDYPLKEELDKIADKFAKQDK
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EAQSAVTAYRPLAKVELPIATGKFATTRYCLMEMQPKTGRKHQLRRHMAHLRHPIVGDTT
HHHHHHHHHCCHHHEEECCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCC
HGDGVHNRLFREHFSAQRLMLHASELRFMHPYTQQPLVIRAGLDEVWQGLLSEFGWQESL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCE
LINA
EECC
>Mature Secondary Structure
MLEIVYQDEYLVAVNKPAGMLVHRSWLDKHETEFVMQTLRDQIGQHVFPLHRLDRPTSGV
CEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCE
LVFALSSQIASEVMPMFANHEMEKTYHAIVRGWIEEANVLDYPLKEELDKIADKFAKQDK
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
EAQSAVTAYRPLAKVELPIATGKFATTRYCLMEMQPKTGRKHQLRRHMAHLRHPIVGDTT
HHHHHHHHHCCHHHEEECCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCC
HGDGVHNRLFREHFSAQRLMLHASELRFMHPYTQQPLVIRAGLDEVWQGLLSEFGWQESL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCE
LINA
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301