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The map label for this gene is aspS

Identifier: 147674215

GI number: 147674215

Start: 762728

End: 764503

Strand: Direct

Name: aspS

Synonym: VC0395_A0735

Alternate gene names: 147674215

Gene position: 762728-764503 (Clockwise)

Preceding gene: 147674623

Following gene: 147674995

Centisome position: 25.22

GC content: 50.17

Gene sequence:

>1776_bases
ATGCGTAGCCATTATTGTGGTCACCTGAACAAGTCCTTGGTAGGACAAACTGTAGAGCTGTGCGGCTGGGTTAATCGCCG
TCGTGATTTAGGCGGTCTGATCTTCATTGATATGCGAGATCGTGAAGGTATTGTTCAGGTAGTAGTGGATCCGGATATGG
CGGATGTATTCGCCGTGGCTAACCAACTGCGTAGCGAATTCTGTATCAAATTGACCGGTGAAGTGCGTGCGCGTCCAGAA
AGCCAAGTCAATAAAGAGATGGCAACCGGTGAAGTAGAACTGTTGGCACGTAGCCTTGAGATCATTAACCGTTCAGATGT
TCTGCCGCTCGATTTCAACCAAAAGAACTCAGAAGAGCAACGCCTCAAGTACCGTTATTTGGATCTGCGTCGCCCAGAGA
TGAGTGACCGCATTAAACTGCGTGCGAAAGCCTCAAGCTTTGTACGCCGTTTCTTGGATACACACGGTTTCCTTGATATC
GAAACCCCAGTGTTGACCAAAGCGACACCAGAAGGTGCGCGTGACTACCTAGTACCAAGCCGTGTACACAAAGGCAGTTT
CTACGCGCTGCCACAATCACCACAGCTGTTTAAACAGCTACTGATGATGTCTGGCTTTGATCGTTATTACCAAATCGTAA
AATGTTTCCGTGACGAAGATTTGCGCGCTGACCGTCAACCAGAATTCACCCAGATCGATATTGAAACCTCTTTCATGACC
GCAGAACAAGTACGCGCAGTGACTGAGAAGATGATTCGTGAAATGTGGCTTGAACTGCTGAATGTGGATCTGGGCGATTT
TCCAATCATGCCTTACAGCGAAGCGATGCGTCGTTTCGGCTCAGACAAGCCAGATCTGCGTAACCCAATGGAGTTGGTGG
ATGTGGCGGATCTGCTGAAAGACGTCGATTTCAAAGTGTTCTCTGGTCCAGCGAACGATCCAAAAGGCCGCGTTGCTGCA
CTGTGCATCCCAGGTGGCGCAGCACTGACTCGTAAACAAATTGATGAGTACACCGCATTTGTGGCTATTTACGGCGCAAA
AGGCTTGGCATGGCTGAAAGTCAACGATTTGGCTGCGGGCATGGAAGGTATTCAATCGCCTGTAGCGAAATTCCTCACTG
AAGAGATCATTCAAGCGATTATCGAGCGTACTCAAGCGCAAACTGGCGATATCATTCTGTTCGGTGCCGATTCTGCCAAA
GTGGTTGCTGAAGCGCTGGGCGCACTGCGTCTGAAAGCGGGTAAAGAGTTGGGTATTACTAATGAATCGGCGTGGGCTCC
ATTGTGGGTCGTGGATTTCCCAATGTTCGAAAGCGATGATGAAGGTAACGTGGCAGCGATGCACCACCCATTCACATCCC
CTCTGAACCTCTCTCCTGAGCAGTTGAAAGCCAACCCAGAAGAGGCGCTGTCAAACGCTTACGATATGGTATTGAACGGC
TACGAAGTCGGTGGTGGTTCAGTCCGTATTCACAACGCAGAAATGCAATCAGCCGTATTTGACATTCTGGGTATCACGCC
AGAAGAGCAACGTCTCAAGTTTGGTTTCCTGCTCGATGCGCTGAAGTTTGGTACGCCACCACACGCAGGTCTGGCATTTG
GTCTGGATCGTCTGGTGATGCTGCTGTGCGGTACGGAAAACATCCGTGATGTCATTGCCTTCCCGAAAACGACAGCCGCC
GCGTGTCTAATGACCGATGCACCAAGTTTGGCAAACCCTGCCGCCCTCGAAGAGTTGGCGATTGCGGTCAAGCTTGCAAC
GAAAGATAAAGCGTAA

Upstream 100 bases:

>100_bases
TCGGCGTATAAATGGAACTTTGCTGAATAGGCAGTCTGTCGAGTACTCCACGACCTTTCAGCGTTAAATTTGATGACTAA
TTGAGATTGGGAAATTCACT

Downstream 100 bases:

>100_bases
TCAACGCCTTGCAAAGGGTGGCATTAGCCACCCTTTTTTTTAACCTGCGCGGCACGAGAGCGCAGACAGTAAAATGATAC
AAACAGGATTTCTTCTTTGG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVANQLRSEFCIKLTGEVRARPE
SQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQRLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLKDVDFKVFSGPANDPKGRVAA
LCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAGMEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAK
VVAEALGALRLKAGKELGITNESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVMLLCGTENIRDVIAFPKTTAA
ACLMTDAPSLANPAALEELAIAVKLATKDKA

Sequences:

>Translated_591_residues
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVANQLRSEFCIKLTGEVRARPE
SQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQRLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLKDVDFKVFSGPANDPKGRVAA
LCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAGMEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAK
VVAEALGALRLKAGKELGITNESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVMLLCGTENIRDVIAFPKTTAA
ACLMTDAPSLANPAALEELAIAVKLATKDKA
>Mature_591_residues
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVANQLRSEFCIKLTGEVRARPE
SQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQRLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDI
ETPVLTKATPEGARDYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLKDVDFKVFSGPANDPKGRVAA
LCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAGMEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAK
VVAEALGALRLKAGKELGITNESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVMLLCGTENIRDVIAFPKTTAA
ACLMTDAPSLANPAALEELAIAVKLATKDKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=39.2384105960265, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI5031815, Length=251, Percent_Identity=27.4900398406374, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI194272210, Length=251, Percent_Identity=27.4900398406374, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI45439306, Length=254, Percent_Identity=25.9842519685039, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI226491502, Length=261, Percent_Identity=22.9885057471264, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788173, Length=583, Percent_Identity=75.8147512864494, Blast_Score=942, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=24.7557003257329, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790571, Length=288, Percent_Identity=25, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=34.21926910299, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=257, Percent_Identity=24.124513618677, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=257, Percent_Identity=24.124513618677, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=257, Percent_Identity=24.124513618677, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=240, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=636, Percent_Identity=34.1194968553459, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=29.6442687747036, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=266, Percent_Identity=25.5639097744361, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323011, Length=163, Percent_Identity=31.2883435582822, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584738, Length=595, Percent_Identity=34.9579831932773, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI24640849, Length=253, Percent_Identity=28.4584980237154, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640851, Length=253, Percent_Identity=28.4584980237154, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136276, Length=258, Percent_Identity=27.1317829457364, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_VIBC3 (A5F263)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F263
- SMR:   A5F263
- STRING:   A5F263
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0735
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   VCHO345073:VC0395_A0735-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65769; Mature: 65769

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
NQLRSEFCIKLTGEVRARPESQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQ
HHHHHHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDIETPVLTKATPEGARDYLVPS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVDFKVFSGPANDPKGRVAALCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAG
HCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHH
MEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAKVVAEALGALRLKAGKELGIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
NESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCC
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVM
EEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLMTDAPSLANPAALEELAIAVKLATKDKA
HHHCCCCHHHHHCCCCHHHHHHEEECCCCCCCHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MRSHYCGHLNKSLVGQTVELCGWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFAVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
NQLRSEFCIKLTGEVRARPESQVNKEMATGEVELLARSLEIINRSDVLPLDFNQKNSEEQ
HHHHHHHEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMSDRIKLRAKASSFVRRFLDTHGFLDIETPVLTKATPEGARDYLVPS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCCCC
RVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSFMT
CCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHH
AEQVRAVTEKMIREMWLELLNVDLGDFPIMPYSEAMRRFGSDKPDLRNPMELVDVADLLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVDFKVFSGPANDPKGRVAALCIPGGAALTRKQIDEYTAFVAIYGAKGLAWLKVNDLAAG
HCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHH
MEGIQSPVAKFLTEEIIQAIIERTQAQTGDIILFGADSAKVVAEALGALRLKAGKELGIT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
NESAWAPLWVVDFPMFESDDEGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNG
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCC
YEVGGGSVRIHNAEMQSAVFDILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVM
EEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLCGTENIRDVIAFPKTTAAACLMTDAPSLANPAALEELAIAVKLATKDKA
HHHCCCCHHHHHCCCCHHHHHHEEECCCCCCCHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA