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The map label for this gene is tag [H]

Identifier: 147674071

GI number: 147674071

Start: 1357575

End: 1358156

Strand: Reverse

Name: tag [H]

Synonym: VC0395_A1277

Alternate gene names: 147674071

Gene position: 1358156-1357575 (Counterclockwise)

Preceding gene: 147674680

Following gene: 147674860

Centisome position: 44.91

GC content: 48.28

Gene sequence:

>582_bases
ATGATGAATGCGGAACAGGGCGTATGTGCTTGGGCGATGAACCATCCCTTAGAGCGTGAATATCACGATAAAGAGTGGGG
CAGAGCTGTGCATGATGATACCAAGCTATTCGAGTTTCTTACATTGGAAGGGGCGCAAGCGGGATTAAGCTGGCTCACGG
TATTGAAAAAGCGCGAAGGGTATCGCCAAGCCTTTTTAGGTTATGATTTACAGCGCCTTGCACAGTGTGATGAAAGCCAT
GTTGAGCAGATCATTGCCGAGTATGATGTCGTAAAGCATCGCGGCAAAATTGCTTCTGTGTTCTCGAATGCGCGAGCCGC
CTTGGCGTTACAACAGGAGTTCGGCTCTTTAGATGCCGCGTTGTGGCAATTTGTCGGTGGTCAACCCAAAATCAACCGCT
GGGTAAGCATGAGCGATGTACCCGCCTCAACGGAAGAATCAAAAGCCATGAGCAAATTTCTGAAAAAACGTGGCTTTAAG
TTTGTGGGCGAAACAATTTGCTATGCCTTTATGCAAGCGTGTGGTTTGGTCAACGACCACTTAGTTGGCTGTCCCAATCA
CTCTAATGCAGACAAGCTCTGA

Upstream 100 bases:

>100_bases
AAGTCCCACAATAGCGATAATGTCAGTGAACGCTGATTAGAGACCTTAAAATACGTGGCTCTTTGGAGCCACGTTGCTCA
TTGAATGAAGAAGGATAAGC

Downstream 100 bases:

>100_bases
TTCACCAAGAGCTGGTTTGGGAAAATACAGAAAATCAAAGCAAATAGGGGCTGAAGCCCCTATTTTGATCATGGAACGAA
AAACTGCTATGCATGCAGGA

Product: DNA-3-methyladenine glycosidase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MMNAEQGVCAWAMNHPLEREYHDKEWGRAVHDDTKLFEFLTLEGAQAGLSWLTVLKKREGYRQAFLGYDLQRLAQCDESH
VEQIIAEYDVVKHRGKIASVFSNARAALALQQEFGSLDAALWQFVGGQPKINRWVSMSDVPASTEESKAMSKFLKKRGFK
FVGETICYAFMQACGLVNDHLVGCPNHSNADKL

Sequences:

>Translated_193_residues
MMNAEQGVCAWAMNHPLEREYHDKEWGRAVHDDTKLFEFLTLEGAQAGLSWLTVLKKREGYRQAFLGYDLQRLAQCDESH
VEQIIAEYDVVKHRGKIASVFSNARAALALQQEFGSLDAALWQFVGGQPKINRWVSMSDVPASTEESKAMSKFLKKRGFK
FVGETICYAFMQACGLVNDHLVGCPNHSNADKL
>Mature_193_residues
MMNAEQGVCAWAMNHPLEREYHDKEWGRAVHDDTKLFEFLTLEGAQAGLSWLTVLKKREGYRQAFLGYDLQRLAQCDESH
VEQIIAEYDVVKHRGKIASVFSNARAALALQQEFGSLDAALWQFVGGQPKINRWVSMSDVPASTEESKAMSKFLKKRGFK
FVGETICYAFMQACGLVNDHLVGCPNHSNADKL

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=176, Percent_Identity=51.7045454545455, Blast_Score=198, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21773; Mature: 21773

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNAEQGVCAWAMNHPLEREYHDKEWGRAVHDDTKLFEFLTLEGAQAGLSWLTVLKKREG
CCCCCCCCHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YRQAFLGYDLQRLAQCDESHVEQIIAEYDVVKHRGKIASVFSNARAALALQQEFGSLDAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWQFVGGQPKINRWVSMSDVPASTEESKAMSKFLKKRGFKFVGETICYAFMQACGLVNDH
HHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCC
LVGCPNHSNADKL
EECCCCCCCCCCC
>Mature Secondary Structure
MMNAEQGVCAWAMNHPLEREYHDKEWGRAVHDDTKLFEFLTLEGAQAGLSWLTVLKKREG
CCCCCCCCHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YRQAFLGYDLQRLAQCDESHVEQIIAEYDVVKHRGKIASVFSNARAALALQQEFGSLDAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWQFVGGQPKINRWVSMSDVPASTEESKAMSKFLKKRGFKFVGETICYAFMQACGLVNDH
HHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCC
LVGCPNHSNADKL
EECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]