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The map label for this gene is hemK [H]

Identifier: 147674049

GI number: 147674049

Start: 1875866

End: 1876726

Strand: Reverse

Name: hemK [H]

Synonym: VC0395_A1755

Alternate gene names: 147674049

Gene position: 1876726-1875866 (Counterclockwise)

Preceding gene: 147674456

Following gene: 147675215

Centisome position: 62.06

GC content: 52.03

Gene sequence:

>861_bases
ATGTCAGTTACCATTGAAGCGGCATTAAAAGCGGCAACCGAGCAACTGCAGCAAAGCGGCAGTGATTCGCCAGCCCTAGA
TGCCGCAGTACTCCTTTGCCATGTACTGGCTAAGCCTCGTTCTTACTTGTTGACTTGGCCTGATAAAATCTTGGAGAAGC
CTACTTTGGCTTCTCTCGAGCTGCTCCTTGCTCGCCGCAGAGCGGGAGAGCCGATGGCCTACATCCTTGGTGAGCGCGAA
TTTTGGTCGCTGCCGCTGAAAGTCTCTCCTTCGACACTTATTCCACGTCCGGATACGGAACGTTTGGTTGAATTAGCGCT
GGATAAAGCGGCGTTGATTGATGGCGAACTGCTGGATCTTGGCACCGGAACTGGGGCGATTGCGTTGGCTTTGGCTTCGG
AATTGCCCACGCGGCAAGTCACGGGGATTGATTTACGTCCGGAGGCGGCAGAGTTAGCAAGAGAAAACGCGACTCGCTTG
GCGATCCACAATGCGCAGTTTTTTCAGGGCAGTTGGTTTAGCCCATTAGCAGACGGTACGAAGTTTGCTTTGATCGTCTC
CAACCCGCCTTATATCGAAGAAAATGATCCGCATTTGAGTCTGGGCGATGTGCGTTTTGAGCCGAAAAGCGCGCTGGTTG
CCGCAGAAAATGGTTTGGCGGACATTCGCCACATCAGCACGCACGCGCCGCGTTTTTTGCTCGATGACGGCTGGCTTTTG
TTTGAGCATGGTTACGATCAAGGCGTTGCGGTGCGCACGATTATGAGTGATTTAGGCTATCAAAACATCATGACCGAGCA
AGATTACGCCGGTCATGATCGAGTTACACTGGGACAATATAAAACGGAAAGAGAAGCATAA

Upstream 100 bases:

>100_bases
GACCGAAGTGATGGAAGGGGATATGCAATCCTTGATTGACCCTGTGATTCACGAGCATCAAGCGGATCAATTGGCGGCAC
TGGCGGATCAAAACTAATCC

Downstream 100 bases:

>100_bases
TGTACGAAGGATTAAAACATTTTCACCTGTTCACTATCGGCGTCAGTGTAGTCATGCTGTCGATTCGCTATGTGCTCATG
ATGATGAATTCAGCACACCT

Product: hemK protein

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGERE
FWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRL
AIHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPRFLLDDGWLL
FEHGYDQGVAVRTIMSDLGYQNIMTEQDYAGHDRVTLGQYKTEREA

Sequences:

>Translated_286_residues
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGERE
FWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRL
AIHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPRFLLDDGWLL
FEHGYDQGVAVRTIMSDLGYQNIMTEQDYAGHDRVTLGQYKTEREA
>Mature_285_residues
SVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGEREF
WSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLA
IHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPRFLLDDGWLLF
EHGYDQGVAVRTIMSDLGYQNIMTEQDYAGHDRVTLGQYKTEREA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=266, Percent_Identity=34.2105263157895, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787463, Length=272, Percent_Identity=53.3088235294118, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI87082085, Length=185, Percent_Identity=32.4324324324324, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=178, Percent_Identity=31.4606741573034, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582226, Length=282, Percent_Identity=32.6241134751773, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31362; Mature: 31231

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLE
CCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHH
LLLARRRAGEPMAYILGEREFWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDL
HHHHHHHCCCCCEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLAIHNAQFFQGSWFSPLADGT
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCC
KFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPRFLLDDGWLL
EEEEEECCCCCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHHCCCEEEECCCEEE
FEHGYDQGVAVRTIMSDLGYQNIMTEQDYAGHDRVTLGQYKTEREA
EECCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLE
CEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHH
LLLARRRAGEPMAYILGEREFWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDL
HHHHHHHCCCCCEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLAIHNAQFFQGSWFSPLADGT
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCC
KFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPRFLLDDGWLL
EEEEEECCCCCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHHCCCEEEECCCEEE
FEHGYDQGVAVRTIMSDLGYQNIMTEQDYAGHDRVTLGQYKTEREA
EECCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]