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The map label for this gene is glyA [H]

Identifier: 147674000

GI number: 147674000

Start: 497534

End: 498841

Strand: Direct

Name: glyA [H]

Synonym: VC0395_A0465

Alternate gene names: 147674000

Gene position: 497534-498841 (Clockwise)

Preceding gene: 147675605

Following gene: 147675601

Centisome position: 16.45

GC content: 49.01

Gene sequence:

>1308_bases
GTGGAGTCATTGTGTGGTTTTTTTGCAATTTCAGTCAATCTGAGTCAGGAGATACAGATGCTTAAGCGTGATATGAACAT
CGCTGATTACGATCCGGAGCTATACGCTGCCATTCAGGAAGAGACGCTTCGTCAAGAAGAACATATTGAACTTATTGCTT
CAGAAAACTACACCAGCCCGCGTGTAATGCAAGCACAAGGTTCTCAACTGACCAACAAGTACGCCGAAGGTTATCCAGGT
AAGCGTTACTATGGTGGTTGTGAGTACGTGGACAAAGCAGAAGCTTTAGCGATTGATCGTGCTTGCCAACTGTTTGGCTG
TGAATACGCGAACGTTCAGCCGCACTCTGGATCTCAAGCGAACAGCGCTGTGTACATGGCGCTGCTCAACCCAAGTGACA
CCGTGCTAGGTATGAGCCTTGCTCACGGTGGTCATTTGACTCACGGTTCACCGGTTAACTTCTCGGGCAAGCACTACAAT
GTGATCCCTTACGGTATCGATGAAGCGGGTCAAATTAACTACGATGAAATGGAAGCCTTAGCACTGGAGCATAAGCCAAA
GATGATCATCGGTGGCTTCTCGGCTTACTCACAAATCGTGGATTGGAAACGCATGCGTGAAATTGCCGATAAAGTGGGTG
CTTACCTGTTTGTTGACATGGCGCACGTTGCGGGTCTGATTGCGGCGGGTGTTTACCCAAGCCCAGTGCCTTTTGCACAC
GTTGTCACGACCACCACGCATAAAACACTGGCAGGTCCACGCGGTGGCTTGATCTTGTCAAATGCCGGTGAGGAGATGTA
CAAGAAGCTGAACTCTGCAGTATTCCCTGGCGGCCAAGGCGGTCCTTTGATGCACGTGATTGCGGCAAAAGCGGTGGCGT
TCAAAGAAGCGATGGAGCCTGAGTTTAAAGAGTATCAAGCGCGTGTGGTGAAAAACGCCAAAGCCATGGTGGCGCAGTTC
CAAGAGCGTGGTTACAAGATCGTCTCTAACAGCACGGAAAACCATTTGTTCCTTGTTGATCTGATTGATAAAAACATCAC
AGGTAAAGAAGCAGATGCCGCTCTGGGCGCTGCCAATATCACGGTTAACAAGAACTCGGTACCAAATGACCCACGCAGCC
CGTTTGTGACTTCAGGTATCCGTGTCGGTACACCTGCAATCACTCGCCGTGGTTTTACTGAGCAAGACGCAAAAGATCTC
GCCAACTGGATGTGTGATGTTCTAGACAACATCAATGATCAAGGTGTGATTGAAGCGACGAAACAAAAAGTATTGGCGAT
CTGTCAACGTCTGCCTGTTTACGCTTAA

Upstream 100 bases:

>100_bases
GTTATGAAAACACTGCATGATAAATTCATGAATTTGGGCTTTATGATAAAGATCAAATTATGTAGAATGCGCGCCATAAA
GTGATGACGAAAACGTTTGC

Downstream 100 bases:

>100_bases
GCGCTAAAACCCCAGCCATGTGAAGTTGCAACGCTATGGTTGCAACTTTCTTTGGTTAGGTTGTCCTGATAAAAAAGCCT
GCGAAGTTCGCAGGCTTTTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1 [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MESLCGFFAISVNLSQEIQMLKRDMNIADYDPELYAAIQEETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPG
KRYYGGCEYVDKAEALAIDRACQLFGCEYANVQPHSGSQANSAVYMALLNPSDTVLGMSLAHGGHLTHGSPVNFSGKHYN
VIPYGIDEAGQINYDEMEALALEHKPKMIIGGFSAYSQIVDWKRMREIADKVGAYLFVDMAHVAGLIAAGVYPSPVPFAH
VVTTTTHKTLAGPRGGLILSNAGEEMYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEAMEPEFKEYQARVVKNAKAMVAQF
QERGYKIVSNSTENHLFLVDLIDKNITGKEADAALGAANITVNKNSVPNDPRSPFVTSGIRVGTPAITRRGFTEQDAKDL
ANWMCDVLDNINDQGVIEATKQKVLAICQRLPVYA

Sequences:

>Translated_435_residues
MESLCGFFAISVNLSQEIQMLKRDMNIADYDPELYAAIQEETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPG
KRYYGGCEYVDKAEALAIDRACQLFGCEYANVQPHSGSQANSAVYMALLNPSDTVLGMSLAHGGHLTHGSPVNFSGKHYN
VIPYGIDEAGQINYDEMEALALEHKPKMIIGGFSAYSQIVDWKRMREIADKVGAYLFVDMAHVAGLIAAGVYPSPVPFAH
VVTTTTHKTLAGPRGGLILSNAGEEMYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEAMEPEFKEYQARVVKNAKAMVAQF
QERGYKIVSNSTENHLFLVDLIDKNITGKEADAALGAANITVNKNSVPNDPRSPFVTSGIRVGTPAITRRGFTEQDAKDL
ANWMCDVLDNINDQGVIEATKQKVLAICQRLPVYA
>Mature_435_residues
MESLCGFFAISVNLSQEIQMLKRDMNIADYDPELYAAIQEETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPG
KRYYGGCEYVDKAEALAIDRACQLFGCEYANVQPHSGSQANSAVYMALLNPSDTVLGMSLAHGGHLTHGSPVNFSGKHYN
VIPYGIDEAGQINYDEMEALALEHKPKMIIGGFSAYSQIVDWKRMREIADKVGAYLFVDMAHVAGLIAAGVYPSPVPFAH
VVTTTTHKTLAGPRGGLILSNAGEEMYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEAMEPEFKEYQARVVKNAKAMVAQF
QERGYKIVSNSTENHLFLVDLIDKNITGKEADAALGAANITVNKNSVPNDPRSPFVTSGIRVGTPAITRRGFTEQDAKDL
ANWMCDVLDNINDQGVIEATKQKVLAICQRLPVYA

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=45.771144278607, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=45.771144278607, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=45.771144278607, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=45.771144278607, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=45.1371571072319, Blast_Score=337, Evalue=9e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=47.6309226932668, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=45.360824742268, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=81.0551558752998, Blast_Score=685, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=44.6384039900249, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=44.6384039900249, Blast_Score=343, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=419, Percent_Identity=43.9140811455847, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=46.0199004975124, Blast_Score=357, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=46.25, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=46, Blast_Score=352, Evalue=2e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 47538; Mature: 47538

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLCGFFAISVNLSQEIQMLKRDMNIADYDPELYAAIQEETLRQEEHIELIASENYTSP
CCCHHEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCC
RVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDKAEALAIDRACQLFGCEYANVQPHSGSQA
CEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NSAVYMALLNPSDTVLGMSLAHGGHLTHGSPVNFSGKHYNVIPYGIDEAGQINYDEMEAL
CCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ALEHKPKMIIGGFSAYSQIVDWKRMREIADKVGAYLFVDMAHVAGLIAAGVYPSPVPFAH
HHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VVTTTTHKTLAGPRGGLILSNAGEEMYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEAMEP
EEEECCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EFKEYQARVVKNAKAMVAQFQERGYKIVSNSTENHLFLVDLIDKNITGKEADAALGAANI
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHCCEEEE
TVNKNSVPNDPRSPFVTSGIRVGTPAITRRGFTEQDAKDLANWMCDVLDNINDQGVIEAT
EECCCCCCCCCCCCCEECCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
KQKVLAICQRLPVYA
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MESLCGFFAISVNLSQEIQMLKRDMNIADYDPELYAAIQEETLRQEEHIELIASENYTSP
CCCHHEEEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHEEEEECCCCCCC
RVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDKAEALAIDRACQLFGCEYANVQPHSGSQA
CEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NSAVYMALLNPSDTVLGMSLAHGGHLTHGSPVNFSGKHYNVIPYGIDEAGQINYDEMEAL
CCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
ALEHKPKMIIGGFSAYSQIVDWKRMREIADKVGAYLFVDMAHVAGLIAAGVYPSPVPFAH
HHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VVTTTTHKTLAGPRGGLILSNAGEEMYKKLNSAVFPGGQGGPLMHVIAAKAVAFKEAMEP
EEEECCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EFKEYQARVVKNAKAMVAQFQERGYKIVSNSTENHLFLVDLIDKNITGKEADAALGAANI
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHCCEEEE
TVNKNSVPNDPRSPFVTSGIRVGTPAITRRGFTEQDAKDLANWMCDVLDNINDQGVIEAT
EECCCCCCCCCCCCCEECCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
KQKVLAICQRLPVYA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]