Click here to switch to the map view.

The map label for this gene is thiL [H]

Identifier: 147673943

GI number: 147673943

Start: 1974586

End: 1975590

Strand: Reverse

Name: thiL [H]

Synonym: VC0395_A1856

Alternate gene names: 147673943

Gene position: 1975590-1974586 (Counterclockwise)

Preceding gene: 147673228

Following gene: 147674430

Centisome position: 65.33

GC content: 50.45

Gene sequence:

>1005_bases
ATGCAGCGTTTCAACCAAAGAGTCTGGATGATGTTTGGTGAATTTAATTTAATCGATAAATACTTTTCCAACCGACAAGC
ACAACGCAAAGACGTTCACTTAGCATTGGGTGATGACTGTGCCATTGTCAAAGTTCCTGAAAATTCGCGCGTGGCGATCA
GCACGGATACCTTAGTTGCTGGAACCCATTTTCTGGCTCAGGCCAATCCGGCTTGGGTCGCGCACAAAGCCTTAGCGTCG
AACATCAGTGATTTGGCGGCGATGGGCGCGACTCCGGCTTGGGTTTCGCTTGCGTTGACTCTGCCTGAGATTGATGATGC
GTGGCTAACGCCATTTTGCGATGCTTTTTTCGAGTTGGCCAAGTACTACAATGTGCAGCTCATTGGTGGCGATACCACCA
AAGGGCCACTCAGTATCACATTAACCGTGCAGGGCTTTTTACCCAAAGAGCAGGCTATGCTGCGTAGCGGGGCGAAAGTT
GGCGATTGGCTCTATGTCACGGGCGATCTAGGCGATAGCCAAGCCGGCTTAGACGTGATTTTAGATCCGGAAAAGCGCCA
TTTACCCTTCGCCGATATTCTTGAGCAGCGCCATTACTTGTCGACACCACGCATTGTGGCAGGGCAAGCCTTAGTGCATT
TGGCCTCTTCGGCGATTGATATTTCCGACGGCCTGATTGCCGATCTGCAACACATTTTGCGGCGTTCCAATGTTGGGGCG
AGTATTGATGTCAGCTTGTTGCCGCTGTCTAAAGAGTTATTGCAGTTTGTAGACAGTGTCACTAGTGCACAGCAGTATGC
CTTGACCAGCGGTGAAGAGTACGAACTCTGCTTCACCATTCCAGAAGAAAATCGAGGCTCGCTAGAAAATGCGCTCGCAC
ATTGCGGCACGAAAGTGACCTGTATTGGCCAAATTCGCCCGGCCGGTACGTTTGAGCTGCACAATCAGGGGAAAAAGCTC
GATTGGCAACTGGCTGGGTATGATCATTTCAAGGTGAAAGCATGA

Upstream 100 bases:

>100_bases
GCTGGATAAAGCCGCACCGCACGTACGTAAGAAAGCGTAATCCGTTTTCCACCAGAAGGTCAGCAGTCGCTGACCTTTTT
TCTTGATAAGCTGCGACCTA

Downstream 100 bases:

>100_bases
GCGATCCACGCAGTAATCTCTCCTTAAGTAACCCTTGGCACTTGCTTGCTACTGGTTTTGGCAGTGGCTTGTCGCCCATC
GTGCCCGGAACGATGGGCAC

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQRFNQRVWMMFGEFNLIDKYFSNRQAQRKDVHLALGDDCAIVKVPENSRVAISTDTLVAGTHFLAQANPAWVAHKALAS
NISDLAAMGATPAWVSLALTLPEIDDAWLTPFCDAFFELAKYYNVQLIGGDTTKGPLSITLTVQGFLPKEQAMLRSGAKV
GDWLYVTGDLGDSQAGLDVILDPEKRHLPFADILEQRHYLSTPRIVAGQALVHLASSAIDISDGLIADLQHILRRSNVGA
SIDVSLLPLSKELLQFVDSVTSAQQYALTSGEEYELCFTIPEENRGSLENALAHCGTKVTCIGQIRPAGTFELHNQGKKL
DWQLAGYDHFKVKA

Sequences:

>Translated_334_residues
MQRFNQRVWMMFGEFNLIDKYFSNRQAQRKDVHLALGDDCAIVKVPENSRVAISTDTLVAGTHFLAQANPAWVAHKALAS
NISDLAAMGATPAWVSLALTLPEIDDAWLTPFCDAFFELAKYYNVQLIGGDTTKGPLSITLTVQGFLPKEQAMLRSGAKV
GDWLYVTGDLGDSQAGLDVILDPEKRHLPFADILEQRHYLSTPRIVAGQALVHLASSAIDISDGLIADLQHILRRSNVGA
SIDVSLLPLSKELLQFVDSVTSAQQYALTSGEEYELCFTIPEENRGSLENALAHCGTKVTCIGQIRPAGTFELHNQGKKL
DWQLAGYDHFKVKA
>Mature_334_residues
MQRFNQRVWMMFGEFNLIDKYFSNRQAQRKDVHLALGDDCAIVKVPENSRVAISTDTLVAGTHFLAQANPAWVAHKALAS
NISDLAAMGATPAWVSLALTLPEIDDAWLTPFCDAFFELAKYYNVQLIGGDTTKGPLSITLTVQGFLPKEQAMLRSGAKV
GDWLYVTGDLGDSQAGLDVILDPEKRHLPFADILEQRHYLSTPRIVAGQALVHLASSAIDISDGLIADLQHILRRSNVGA
SIDVSLLPLSKELLQFVDSVTSAQQYALTSGEEYELCFTIPEENRGSLENALAHCGTKVTCIGQIRPAGTFELHNQGKKL
DWQLAGYDHFKVKA

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=325, Percent_Identity=54.1538461538462, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 36683; Mature: 36683

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFNQRVWMMFGEFNLIDKYFSNRQAQRKDVHLALGDDCAIVKVPENSRVAISTDTLVA
CCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCEEEEECCCEEE
GTHFLAQANPAWVAHKALASNISDLAAMGATPAWVSLALTLPEIDDAWLTPFCDAFFELA
CHHHEECCCCHHHHHHHHHHCHHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHH
KYYNVQLIGGDTTKGPLSITLTVQGFLPKEQAMLRSGAKVGDWLYVTGDLGDSQAGLDVI
HHEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEE
LDPEKRHLPFADILEQRHYLSTPRIVAGQALVHLASSAIDISDGLIADLQHILRRSNVGA
ECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
SIDVSLLPLSKELLQFVDSVTSAQQYALTSGEEYELCFTIPEENRGSLENALAHCGTKVT
EEEEEEECCHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHCCCEEE
CIGQIRPAGTFELHNQGKKLDWQLAGYDHFKVKA
EEEECCCCCEEEECCCCCEEEEEEECCCEEEEEC
>Mature Secondary Structure
MQRFNQRVWMMFGEFNLIDKYFSNRQAQRKDVHLALGDDCAIVKVPENSRVAISTDTLVA
CCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCEEEEECCCEEE
GTHFLAQANPAWVAHKALASNISDLAAMGATPAWVSLALTLPEIDDAWLTPFCDAFFELA
CHHHEECCCCHHHHHHHHHHCHHHHHHCCCCCHHEEEEEECCCCCCCHHHHHHHHHHHHH
KYYNVQLIGGDTTKGPLSITLTVQGFLPKEQAMLRSGAKVGDWLYVTGDLGDSQAGLDVI
HHEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEE
LDPEKRHLPFADILEQRHYLSTPRIVAGQALVHLASSAIDISDGLIADLQHILRRSNVGA
ECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
SIDVSLLPLSKELLQFVDSVTSAQQYALTSGEEYELCFTIPEENRGSLENALAHCGTKVT
EEEEEEECCHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHCCCEEE
CIGQIRPAGTFELHNQGKKLDWQLAGYDHFKVKA
EEEECCCCCEEEECCCCCEEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]