Click here to switch to the map view.

The map label for this gene is gph [H]

Identifier: 147673858

GI number: 147673858

Start: 2349288

End: 2349968

Strand: Reverse

Name: gph [H]

Synonym: VC0395_A2201

Alternate gene names: 147673858

Gene position: 2349968-2349288 (Counterclockwise)

Preceding gene: 147674230

Following gene: 147673103

Centisome position: 77.71

GC content: 50.22

Gene sequence:

>681_bases
TTGAAATCCATAAAATTAATTGCTTTTGATTTGGATGGTACGCTGCTCGACAGTGTGCCGGATTTAGCGGTAGCGGCGGA
TCAAGCGGCGCGTGCGGTCAGCTATCCGGCGGTGAGCGAAGCGCAGGTGCGTGATTATGTCGGCAACGGTGCGGATGTGT
TGATTGCGCGCGCACTCAGCCAGAGCTTAACGATCAATCCTGAATTAAGCCCAGAGCTACGTGCTCAAGCGCGTCACCTG
TTTGATGAGTTTTATGAACAAACGGGGCACAAGCTGAGCCACCTGTATCCTAATGTTAAAACCACGCTTTTAGAGCTGCA
TCAAGCGGGCTTTATTCTGGCGCTCGTGACCAATAAGCCTTCTAAATTTGTGCCAGATGTTCTAGAGCAACACGGCATCG
CGCACTTTTTCAGTGATGTGATTGGCGGCGATACCTTCCCGAATAAAAAGCCGGATCCGATGGCGCTGAATTGGTTATTA
GAGAAGCATCAGTTAAGCGCTGAGCAAATGTTGATGGTCGGGGATTCGAAAAACGACATTCTGGCGGCAAAAAATGCTGG
GTGTTATTCCTTCGGTTTAACCTACGGTTATAACCACGGCGAACCGATTGCCAATGCGGAGCCAGACTTCGTCTCCGATG
ATATTGGCACGCTGCTTGAAGTGGTGCTCGTTTCGGCGTAA

Upstream 100 bases:

>100_bases
GGCTCGGCGATTTTTGGTCAGCCTGATTACCAAGCGGTGATTGAGCAGATGCGTGCTGAACTGGCGAAAGCCAAAATTTA
ATCTGATTTAGGATCGTGTT

Downstream 100 bases:

>100_bases
AAGTTGCCGTTCGCACTAGTAAAATACTCGTTAATGAGTACACTGCTTGAACCGCGCCGATTCCGTGCGCGGTTTTTGTC
CCTTTGTTTTGTGTAATTTC

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKSIKLIAFDLDGTLLDSVPDLAVAADQAARAVSYPAVSEAQVRDYVGNGADVLIARALSQSLTINPELSPELRAQARHL
FDEFYEQTGHKLSHLYPNVKTTLLELHQAGFILALVTNKPSKFVPDVLEQHGIAHFFSDVIGGDTFPNKKPDPMALNWLL
EKHQLSAEQMLMVGDSKNDILAAKNAGCYSFGLTYGYNHGEPIANAEPDFVSDDIGTLLEVVLVSA

Sequences:

>Translated_226_residues
MKSIKLIAFDLDGTLLDSVPDLAVAADQAARAVSYPAVSEAQVRDYVGNGADVLIARALSQSLTINPELSPELRAQARHL
FDEFYEQTGHKLSHLYPNVKTTLLELHQAGFILALVTNKPSKFVPDVLEQHGIAHFFSDVIGGDTFPNKKPDPMALNWLL
EKHQLSAEQMLMVGDSKNDILAAKNAGCYSFGLTYGYNHGEPIANAEPDFVSDDIGTLLEVVLVSA
>Mature_226_residues
MKSIKLIAFDLDGTLLDSVPDLAVAADQAARAVSYPAVSEAQVRDYVGNGADVLIARALSQSLTINPELSPELRAQARHL
FDEFYEQTGHKLSHLYPNVKTTLLELHQAGFILALVTNKPSKFVPDVLEQHGIAHFFSDVIGGDTFPNKKPDPMALNWLL
EKHQLSAEQMLMVGDSKNDILAAKNAGCYSFGLTYGYNHGEPIANAEPDFVSDDIGTLLEVVLVSA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=45.6896551724138, Blast_Score=184, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24590; Mature: 24590

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIKLIAFDLDGTLLDSVPDLAVAADQAARAVSYPAVSEAQVRDYVGNGADVLIARALS
CCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHH
QSLTINPELSPELRAQARHLFDEFYEQTGHKLSHLYPNVKTTLLELHQAGFILALVTNKP
HCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCEEEEEEECCC
SKFVPDVLEQHGIAHFFSDVIGGDTFPNKKPDPMALNWLLEKHQLSAEQMLMVGDSKNDI
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCE
LAAKNAGCYSFGLTYGYNHGEPIANAEPDFVSDDIGTLLEVVLVSA
EEECCCCEEEECCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSIKLIAFDLDGTLLDSVPDLAVAADQAARAVSYPAVSEAQVRDYVGNGADVLIARALS
CCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHH
QSLTINPELSPELRAQARHLFDEFYEQTGHKLSHLYPNVKTTLLELHQAGFILALVTNKP
HCEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCEEEEEEECCC
SKFVPDVLEQHGIAHFFSDVIGGDTFPNKKPDPMALNWLLEKHQLSAEQMLMVGDSKNDI
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCE
LAAKNAGCYSFGLTYGYNHGEPIANAEPDFVSDDIGTLLEVVLVSA
EEECCCCEEEECCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]