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The map label for this gene is aroA

Identifier: 147673856

GI number: 147673856

Start: 1423637

End: 1424917

Strand: Reverse

Name: aroA

Synonym: VC0395_A1334

Alternate gene names: 147673856

Gene position: 1424917-1423637 (Counterclockwise)

Preceding gene: 229259763

Following gene: 147675774

Centisome position: 47.12

GC content: 50.51

Gene sequence:

>1281_bases
ATGGAAAGCTTGACTCTACAACCGATTGAACTCATCTCTGGGGAAGTGAATCTTCCCGGTTCCAAAAGCGTTTCAAACCG
TGCGCTCTTGCTGGCTGCGCTAGCCTCAGGCACGACTCGTCTTACTAACTTGCTCGATAGCGACGATATTCGCCATATGC
TCAATGCTTTGACCAAGCTGGGTGTGAACTATCGCCTCTCGGCCGATAAAACCACCTGTGAAGTAGAAGGTTTGGGCCAA
GCCTTTCACACGACTCAGCCATTAGAGCTGTTTCTAGGTAACGCAGGTACTGCAATGCGTCCGCTGGCGGCGGCGTTGTG
TCTTGGACAAGGCGACTATGTACTGACTGGCGAACCGCGCATGAAAGAGCGCCCGATTGGCCACTTAGTGGATGCTCTTC
GTCAAGCCAGCGCACAGATTGAGTATCTGGAGCAGGAAAACTTTCCTCCACTGCGTATTCAAGGGACGGGCTTACAAGCA
GGAACGGTGACTATCGATGGTTCTATCTCTAGTCAGTTTTTGACCGCCTTTCTTATGTCGGCACCGTTGGCGCAGGGCAA
AGTGACCATCAAGATCGTCGGTGAGCTGGTTTCTAAGCCTTACATCGACATTACACTGCACATCATGGAGCAGTTTGGTG
TTCAGGTGATCAACCACGATTATCAAGAATTTGTGATCCCAGCGGGGCAATCTTATGTGTCTCCGGGGCAGTTCCTCGTC
GAAGGTGATGCCTCTTCTGCTTCCTATTTCCTTGCTGCGGCTGCCATTAAAGGCGGTGAGGTAAAAGTGACCGGTATTGG
TAAAAACAGCATCCAAGGGGATATTCAATTTGCGGATGCATTAGAAAAGATGGGCGCGCAAATTGAGTGGGGCGATGATT
ATGTGATTGCTCGCCGTGGTGAACTGAATGCGGTGGATCTCGATTTTAACCATATCCCAGATGCGGCGATGACGATTGCG
ACGACGGCACTTTTTGCCAAAGGTACCACGGCCATTCGTAACGTTTACAACTGGCGTGTAAAAGAGACGGATCGCTTGGC
AGCAATGGCCACCGAACTGCGTAAAGTGGGCGCGACAGTCGAAGAGGGGGAAGATTTCATTGTGATTACGCCTCCAACTA
AGCTCATCCATGCGGCAATCGATACCTATGACGATCACCGGATGGCGATGTGTTTTTCTCTGGTTGCGTTGAGCGATACA
CCAGTGACGATCAATGACCCGAAATGCACGTCAAAAACGTTCCCCGATTACTTTGATAAGTTTGCGCAATTAAGCCGCTA
A

Upstream 100 bases:

>100_bases
GCGCTAGCGCATGAATTTGGTTAGTCTGCGGATACGAAGAATTTTCGCCGCGACCCATTACCACAAGGACTTTCAGCGGC
GCAAGGAAACACAACATCCA

Downstream 100 bases:

>100_bases
AGTGTCACTCCCTGCCCAAAACCGATGGCGATAAAATCGGTTTTGGAGTAGATGTAAGTGATTGAAAAAAGCCTCATTAC
GAGGCTTTTATTATTTTTGT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKLGVNYRLSADKTTCEVEGLGQ
AFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPRMKERPIGHLVDALRQASAQIEYLEQENFPPLRIQGTGLQA
GTVTIDGSISSQFLTAFLMSAPLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV
EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRGELNAVDLDFNHIPDAAMTIA
TTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATVEEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDT
PVTINDPKCTSKTFPDYFDKFAQLSR

Sequences:

>Translated_426_residues
MESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKLGVNYRLSADKTTCEVEGLGQ
AFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPRMKERPIGHLVDALRQASAQIEYLEQENFPPLRIQGTGLQA
GTVTIDGSISSQFLTAFLMSAPLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV
EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRGELNAVDLDFNHIPDAAMTIA
TTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATVEEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDT
PVTINDPKCTSKTFPDYFDKFAQLSR
>Mature_426_residues
MESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKLGVNYRLSADKTTCEVEGLGQ
AFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPRMKERPIGHLVDALRQASAQIEYLEQENFPPLRIQGTGLQA
GTVTIDGSISSQFLTAFLMSAPLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV
EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRGELNAVDLDFNHIPDAAMTIA
TTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATVEEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDT
PVTINDPKCTSKTFPDYFDKFAQLSR

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=426, Percent_Identity=75.1173708920188, Blast_Score=647, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320332, Length=443, Percent_Identity=37.9232505643341, Blast_Score=247, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_VIBC3 (A5F7G5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F7G5
- SMR:   A5F7G5
- STRING:   A5F7G5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1334
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- BioCyc:   VCHO345073:VC0395_A1334-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46132; Mature: 46132

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKL
CCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYRLSADKTTCEVEGLGQAFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPR
CCCEEEECCCCEEEEHHCCHHHCCCCCHHEEECCCCCHHHHHHHHHHCCCCCEEEECCCC
MKERPIGHLVDALRQASAQIEYLEQENFPPLRIQGTGLQAGTVTIDGSISSQFLTAFLMS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECEEEECCCCHHHHHHHHHHH
APLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV
CCCCCCCEEEEEEHHHHCCCCEEEHHHHHHHHCHHHHCCCCHHEEEECCCCCCCCCCEEE
EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRG
ECCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECC
ELNAVDLDFNHIPDAAMTIATTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATV
CCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEECCHHHHHHHHHHHHHHCCHH
EEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDTPVTINDPKCTSKTFPDYFDK
CCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHH
FAQLSR
HHHHCC
>Mature Secondary Structure
MESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKL
CCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYRLSADKTTCEVEGLGQAFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPR
CCCEEEECCCCEEEEHHCCHHHCCCCCHHEEECCCCCHHHHHHHHHHCCCCCEEEECCCC
MKERPIGHLVDALRQASAQIEYLEQENFPPLRIQGTGLQAGTVTIDGSISSQFLTAFLMS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEECEEEECCCCHHHHHHHHHHH
APLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV
CCCCCCCEEEEEEHHHHCCCCEEEHHHHHHHHCHHHHCCCCHHEEEECCCCCCCCCCEEE
EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRG
ECCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEECC
ELNAVDLDFNHIPDAAMTIATTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATV
CCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEECCHHHHHHHHHHHHHHCCHH
EEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDTPVTINDPKCTSKTFPDYFDK
CCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHH
FAQLSR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA