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The map label for this gene is hlyB [H]

Identifier: 147673794

GI number: 147673794

Start: 935994

End: 937625

Strand: Reverse

Name: hlyB [H]

Synonym: VC0395_A0916

Alternate gene names: 147673794

Gene position: 937625-935994 (Counterclockwise)

Preceding gene: 147675128

Following gene: 147673141

Centisome position: 31.01

GC content: 46.14

Gene sequence:

>1632_bases
ATGAACTCATCCATTCTTAGTAAATTGCTGCTGACATTAATCACCGTGTTTGCCTTAGTTTTGGCGGGCTCAACGTCTTA
TCAATATTGGCAGCAAAAAGATCTCATTCATTCCGTGTTAAGTGAGCAGCTGCACGACAAAGCCAGTAACTACTTTGATA
GCCTGAACATGATGATGCTGACTGGCACCATGGCACAAAAAGAGACGCTACGCCAAAAAGCATTAGCACAAGATGGCATT
GAACAAGTTCGCGTCATTCGAGCGCCTTCTTTAAACAAGATTTATGGCCCCGGAAGCACGGATCAACAACCGAAAGATGA
AATTGATCAACGGGCATTACAAGGCGAAACCATTATAGAGCCCTATCAAGCCGATTGGGGGAAAGGCATTGTGGTTGCCT
TGCCGATGAAGTCGAGTGAAAACTATCGTGGTACTAACTGTGTGGCTTGTCACATCGCACCAGAAGGTGAAGTGCTTGGT
GTAATTCGTCTTGAGTTTAATCTTAACCACTTAACGCAAATGGTGAATCATCGCACTCTTATTGGTGTCGGCATTATGTC
GGTTATTGCGCTGTTTGGTTTTATGTTGACGCTGTTTTTCATGCGTAGAATTATCGTTCTCCCTTTGCAAAAAACGTCGG
CCTTTATGTGTGAAGTGAGTAAGAGTAAAGATCTTTCCCATCGACTCGAAAACGATAGAAAAGATGAACTCGGGCAACTG
GCGGTATCCATCAACTCACTGATGGATACGGTGTCAGACAGCTTGCAACAAGTGCAAGACACTTCCCATTCCCTTGCTGA
TTCGGCCAAAAAGCTTACGAATGTCGCGCAAGTCACCGATCAGGCGGCCAACAATCAACAAAAAGAAACTGCAGAAGTGC
AAACCAACATCACGGATATGCAAGGTAAGCAGCAACAAGTGGAGTTTGCGACCGAAGAAGCGTCTAAATTGGTCAATCAC
ACCACCAGTATTGTTCAGCAAAGTGCCAAGCAAGCACACGCAGCCAGTGAAGATATCAAAAATCTGGTGGGCGATATTGA
GGCGGTCAAACAACGGATTGTGGAATTAAATAATCAAACTGGCCAAGTATCATCCATTCTTGGCGTGATCAAAGGCATTG
CCGAACAAACTAACTTACTTGCGCTGAATGCCGCTATTGAGGCGGCGCGAGCGGGCGAACAAGGTCGCGGTTTTGCTGTC
GTTGCAGATGAAGTGCGTCATCTTGCATCACGCACCACTGAAGCAACCGGAAATATTGAAAACATCATCGCAGGATTTCA
GCGTGACAGCCAAGCCTCGCTCTCTTCGGTAGATACCGTCTGCTCTCATGCGCATCAGCGTTCATCCGAAGTGGAAGAGC
TCTCAGCGGCCATGACTCGTGTGGTCAGCGAAATGCAACAAGTGCTAAGCCATGCTATGGGCATTCAAGAGCAGACCAAA
CGCACCACGGAAGTCAGTCATGAGATCCAAAATAAGGTTGATGTCATCACTCATCACGCGAACAACACATCACAATCGGC
AAGTGAGACGCGTGAGATCAGCATCGATCTTGAACATTTGTCACAACAATTGGAGTCCTTGCTGAATCAATTTACTTTGA
TTGCTAAGAATTCTGCGCGGAAACCACATTAA

Upstream 100 bases:

>100_bases
CTTTGGTTAAGGTCACACTAACAATTGGTCTACGTTTGTCACCTAGTGAATTAACGCTATATTTACATAAGTCGGGGTCA
CTTATAATTACAAAACTATC

Downstream 100 bases:

>100_bases
AGGTTGAAGCTGACAAATTGACAGGTAATATGTTCCGTTGAATCTGTAAAACTCTCTACAACCCAATGTTTCGTCTATTT
TTTGCGCAATGACGAGCGCA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MNSSILSKLLLTLITVFALVLAGSTSYQYWQQKDLIHSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVIRAPSLNKIYGPGSTDQQPKDEIDQRALQGETIIEPYQADWGKGIVVALPMKSSENYRGTNCVACHIAPEGEVLG
VIRLEFNLNHLTQMVNHRTLIGVGIMSVIALFGFMLTLFFMRRIIVLPLQKTSAFMCEVSKSKDLSHRLENDRKDELGQL
AVSINSLMDTVSDSLQQVQDTSHSLADSAKKLTNVAQVTDQAANNQQKETAEVQTNITDMQGKQQQVEFATEEASKLVNH
TTSIVQQSAKQAHAASEDIKNLVGDIEAVKQRIVELNNQTGQVSSILGVIKGIAEQTNLLALNAAIEAARAGEQGRGFAV
VADEVRHLASRTTEATGNIENIIAGFQRDSQASLSSVDTVCSHAHQRSSEVEELSAAMTRVVSEMQQVLSHAMGIQEQTK
RTTEVSHEIQNKVDVITHHANNTSQSASETREISIDLEHLSQQLESLLNQFTLIAKNSARKPH

Sequences:

>Translated_543_residues
MNSSILSKLLLTLITVFALVLAGSTSYQYWQQKDLIHSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVIRAPSLNKIYGPGSTDQQPKDEIDQRALQGETIIEPYQADWGKGIVVALPMKSSENYRGTNCVACHIAPEGEVLG
VIRLEFNLNHLTQMVNHRTLIGVGIMSVIALFGFMLTLFFMRRIIVLPLQKTSAFMCEVSKSKDLSHRLENDRKDELGQL
AVSINSLMDTVSDSLQQVQDTSHSLADSAKKLTNVAQVTDQAANNQQKETAEVQTNITDMQGKQQQVEFATEEASKLVNH
TTSIVQQSAKQAHAASEDIKNLVGDIEAVKQRIVELNNQTGQVSSILGVIKGIAEQTNLLALNAAIEAARAGEQGRGFAV
VADEVRHLASRTTEATGNIENIIAGFQRDSQASLSSVDTVCSHAHQRSSEVEELSAAMTRVVSEMQQVLSHAMGIQEQTK
RTTEVSHEIQNKVDVITHHANNTSQSASETREISIDLEHLSQQLESLLNQFTLIAKNSARKPH
>Mature_543_residues
MNSSILSKLLLTLITVFALVLAGSTSYQYWQQKDLIHSVLSEQLHDKASNYFDSLNMMMLTGTMAQKETLRQKALAQDGI
EQVRVIRAPSLNKIYGPGSTDQQPKDEIDQRALQGETIIEPYQADWGKGIVVALPMKSSENYRGTNCVACHIAPEGEVLG
VIRLEFNLNHLTQMVNHRTLIGVGIMSVIALFGFMLTLFFMRRIIVLPLQKTSAFMCEVSKSKDLSHRLENDRKDELGQL
AVSINSLMDTVSDSLQQVQDTSHSLADSAKKLTNVAQVTDQAANNQQKETAEVQTNITDMQGKQQQVEFATEEASKLVNH
TTSIVQQSAKQAHAASEDIKNLVGDIEAVKQRIVELNNQTGQVSSILGVIKGIAEQTNLLALNAAIEAARAGEQGRGFAV
VADEVRHLASRTTEATGNIENIIAGFQRDSQASLSSVDTVCSHAHQRSSEVEELSAAMTRVVSEMQQVLSHAMGIQEQTK
RTTEVSHEIQNKVDVITHHANNTSQSASETREISIDLEHLSQQLESLLNQFTLIAKNSARKPH

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=550, Percent_Identity=28.3636363636364, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788195, Length=279, Percent_Identity=32.258064516129, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI2367378, Length=302, Percent_Identity=30.1324503311258, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788194, Length=358, Percent_Identity=28.2122905027933, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789453, Length=305, Percent_Identity=30.1639344262295, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59759; Mature: 59759

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSILSKLLLTLITVFALVLAGSTSYQYWQQKDLIHSVLSEQLHDKASNYFDSLNMMML
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTMAQKETLRQKALAQDGIEQVRVIRAPSLNKIYGPGSTDQQPKDEIDQRALQGETIIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PYQADWGKGIVVALPMKSSENYRGTNCVACHIAPEGEVLGVIRLEFNLNHLTQMVNHRTL
CCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECHHHHHHHHHHHHH
IGVGIMSVIALFGFMLTLFFMRRIIVLPLQKTSAFMCEVSKSKDLSHRLENDRKDELGQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHH
AVSINSLMDTVSDSLQQVQDTSHSLADSAKKLTNVAQVTDQAANNQQKETAEVQTNITDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHC
QGKQQQVEFATEEASKLVNHTTSIVQQSAKQAHAASEDIKNLVGDIEAVKQRIVELNNQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GQVSSILGVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLASRTTEATGNIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCHHCCCCHH
NIIAGFQRDSQASLSSVDTVCSHAHQRSSEVEELSAAMTRVVSEMQQVLSHAMGIQEQTK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RTTEVSHEIQNKVDVITHHANNTSQSASETREISIDLEHLSQQLESLLNQFTLIAKNSAR
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCC
KPH
CCC
>Mature Secondary Structure
MNSSILSKLLLTLITVFALVLAGSTSYQYWQQKDLIHSVLSEQLHDKASNYFDSLNMMML
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTMAQKETLRQKALAQDGIEQVRVIRAPSLNKIYGPGSTDQQPKDEIDQRALQGETIIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PYQADWGKGIVVALPMKSSENYRGTNCVACHIAPEGEVLGVIRLEFNLNHLTQMVNHRTL
CCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECHHHHHHHHHHHHH
IGVGIMSVIALFGFMLTLFFMRRIIVLPLQKTSAFMCEVSKSKDLSHRLENDRKDELGQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHH
AVSINSLMDTVSDSLQQVQDTSHSLADSAKKLTNVAQVTDQAANNQQKETAEVQTNITDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHC
QGKQQQVEFATEEASKLVNHTTSIVQQSAKQAHAASEDIKNLVGDIEAVKQRIVELNNQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GQVSSILGVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLASRTTEATGNIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCHHCCCCHH
NIIAGFQRDSQASLSSVDTVCSHAHQRSSEVEELSAAMTRVVSEMQQVLSHAMGIQEQTK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RTTEVSHEIQNKVDVITHHANNTSQSASETREISIDLEHLSQQLESLLNQFTLIAKNSAR
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCC
KPH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]