Click here to switch to the map view.

The map label for this gene is vibE [H]

Identifier: 147673711

GI number: 147673711

Start: 317892

End: 319523

Strand: Reverse

Name: vibE [H]

Synonym: VC0395_A0301

Alternate gene names: 147673711

Gene position: 319523-317892 (Counterclockwise)

Preceding gene: 147675125

Following gene: 147675003

Centisome position: 10.57

GC content: 50.61

Gene sequence:

>1632_bases
ATGACAACCGATTTTACCCCTTGGCCAGAGGCACTTGCCGCTCAATACCGTCAGTTAGGCTACTGGCAAGATAAAACCTT
GCTCGATTACTTACAGCAAAGTGCTGAGCGTACCCCCAATGCGCTTGCGCTGGTCGGTGATAATCAGCAGTGGCGTTACC
AAGCCATGCTCGAACGTATTGAGCAGCTTGCTGCCGGATTTACCGAACTTGGGCTAGGCTGTGGCGATAACGTAGTGCTG
CAATTAGGCAATGTTGCGGAATTCTATTTATGCTTTTTCGCTTTGCTGCGCCAAGGCATACGACCGATTCTGGCGCTCCC
CGCTCACCGTTTAGCGGAAATTCGCTATTTTTGTCAGCACAGCCAAGCCAAAGCTTACCTCATTGATGGCGCTCAGCGAC
CATTTGACTATCAAGCTTTGGCGCAAGAGCTGCTTGCTTGCTGCCCAACACTGCAGACTGTGATTGTACGCGGCCAAACT
CGAGTGACAGATCCAAAATTTATTGAACTGGCGAGTTGCTACTCGGCATCATCTTGTCAAGCGAATGCCGACCCGAATCA
AATCGCCTTTTTCCAACTTTCCGGTGGCACAACGGACACGCCCAAGCTCATCCCGCGTACCCACAATGATTACGCCTACA
GCGTAACCGCCAGTGTCGAAATTTGCCGTTTTGATCAGCATACTCGCTATCTGTGCGTACTGCCCGCGGCACATAACTTC
CCTCTCAGCTCCCCCGGCGCACTCGGTGTTTTCTGGGCGGGTGGCTGCGTTGTCCTCAGTCAAGATGCGTCACCCCAACA
CGCGTTTAAATTGATAGAACAGCATAAAATTACTGTTACCGCGTTGGTGCCGCCTTTAGCACTACTCTGGATGGATCACG
CAGAAAAAAGCACCTACGATCTCTCCTCATTGCACTTTGTGCAAGTAGGCGGGGCAAAATTTAGTGAAGCGGCCGCAAGG
CGTCTGCCGAAAGCGCTAGGCTGCCAATTGCAACAAGTGTTTGGAATGGCAGAAGGCTTGGTCAATTACACTCGTTTGGA
TGACAGTGCGGAACTGATTGCTACAACTCAAGGTCGGCCTATCTCCGCTCACGATCAATTACTGGTGGTTGATGAGCAGG
GTCAACCTGTCGCCAGCGGAGAAGAAGGGTATCTGTTAACCCAAGGTCCCTATACGATTCGCGGTTACTATCGAGCTGAT
CAGCACAATCAACGAGCGTTCAATGCACAAGGTTTTTACATCACCGGCGATAAAGTCAAACTCAGTAGCGAAGGTTACGT
GATTGTCACCGGAAGGGCCAAAGATCAGATCAATCGTGGAGGGGAAAAGATTGCCGCCGAAGAGGTGGAAAATCAGCTTT
TACATCATCCAGCAGTGCATGATGCCGCTTTAATTGCCATCAGTGACGAATACCTAGGAGAGCGCAGTTGCGCGGTGATC
GTACTCAAACCCGAGCAGTCGGTGAATACCATCCAGCTCAAACGTTTTTTACACCAAGCGGGGCTCGCCGATTACAAAAT
CCCAGATCAAATCCAATTTATCGATCAACTGCCCAAAACATCGGTTGGAAAAATCGATAAGAACGCGCTTCGTCGTCGAT
TTGACACGCTCGGCTTAGCGTTAATGTCGTGA

Upstream 100 bases:

>100_bases
CCACAAAGTGAACTGGAAGAGACGGGCGCGAAGATGAAAACCATCTTGGCTGCGGCAGGTATTGAGCTCAACGATGTGCT
CACCGCTTAAGGAGATAAGA

Downstream 100 bases:

>100_bases
AATCAATGTAAAGACAATGAGTGAGTAGAGGGAATCCTTGAATAAGCGGTTCCCTTCCTACTTAGAATCCGTGTGTTAAG
CAGGAAGGGAACTCGTTCAA

Product: vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVL
QLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQT
RVTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTDTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAAR
RLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRAD
QHNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS

Sequences:

>Translated_543_residues
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVL
QLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQT
RVTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTDTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAAR
RLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRAD
QHNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS
>Mature_542_residues
TTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERIEQLAAGFTELGLGCGDNVVLQ
LGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQHSQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTR
VTDPKFIELASCYSASSCQANADPNQIAFFQLSGGTTDTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNFP
LSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYDLSSLHFVQVGGAKFSEAAARR
LPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRPISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQ
HNQRAFNAQGFYITGDKVKLSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVIV
LKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLALMS

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate, preparing that molecule for the final stages of vibriobactin synthesis [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI115511026, Length=556, Percent_Identity=27.3381294964029, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI38505220, Length=527, Percent_Identity=24.8576850094877, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI42544132, Length=555, Percent_Identity=25.2252252252252, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761345, Length=390, Percent_Identity=28.974358974359, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI187761343, Length=390, Percent_Identity=28.974358974359, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=23.5507246376812, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=26.0952380952381, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157311624, Length=540, Percent_Identity=25, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI157311622, Length=540, Percent_Identity=25, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI58082049, Length=545, Percent_Identity=24.954128440367, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI28416953, Length=547, Percent_Identity=23.9488117001828, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI195947378, Length=412, Percent_Identity=22.3300970873786, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI8923896, Length=158, Percent_Identity=30.379746835443, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI253970489, Length=158, Percent_Identity=30.379746835443, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=538, Percent_Identity=47.9553903345725, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=30.6818181818182, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=25.3731343283582, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=26.0956175298805, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790505, Length=366, Percent_Identity=24.5901639344262, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789201, Length=371, Percent_Identity=24.2587601078167, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786529, Length=553, Percent_Identity=21.880650994575, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788595, Length=495, Percent_Identity=23.4343434343434, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=26.9662921348315, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=514, Percent_Identity=25.0972762645914, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=24.7104247104247, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=23.7226277372263, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=23.8532110091743, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=23.8532110091743, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=23.8532110091743, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=23.5294117647059, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=21.8568665377176, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=23.9782016348774, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=551, Percent_Identity=22.6860254083485, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=551, Percent_Identity=22.6860254083485, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=523, Percent_Identity=22.7533460803059, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=315, Percent_Identity=25.0793650793651, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=485, Percent_Identity=22.8865979381443, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=21.6457960644007, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=26.8738574040219, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=22.9571984435798, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355181, Length=541, Percent_Identity=23.4750462107209, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922652, Length=354, Percent_Identity=24.5762711864407, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24667955, Length=532, Percent_Identity=23.6842105263158, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=23.6842105263158, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648260, Length=283, Percent_Identity=22.9681978798587, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667959, Length=366, Percent_Identity=24.8633879781421, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24656500, Length=490, Percent_Identity=23.265306122449, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356947, Length=294, Percent_Identity=22.108843537415, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365686, Length=292, Percent_Identity=23.2876712328767, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=280, Percent_Identity=22.5, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356441, Length=476, Percent_Identity=21.6386554621849, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 60124; Mature: 59993

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH
HHHHCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHC
SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ
CCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
ANADPNQIAFFQLSGGTTDTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCC
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD
CCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCC
LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP
CCCEEEEEECCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK
CCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEECCCCEEEECCEEE
LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
ECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCEEEE
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LMS
HCC
>Mature Secondary Structure 
TTDFTPWPEALAAQYRQLGYWQDKTLLDYLQQSAERTPNALALVGDNQQWRYQAMLERI
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
EQLAAGFTELGLGCGDNVVLQLGNVAEFYLCFFALLRQGIRPILALPAHRLAEIRYFCQH
HHHHCCHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHC
SQAKAYLIDGAQRPFDYQALAQELLACCPTLQTVIVRGQTRVTDPKFIELASCYSASSCQ
CCCCEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
ANADPNQIAFFQLSGGTTDTPKLIPRTHNDYAYSVTASVEICRFDQHTRYLCVLPAAHNF
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCC
PLSSPGALGVFWAGGCVVLSQDASPQHAFKLIEQHKITVTALVPPLALLWMDHAEKSTYD
CCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCCCCCCC
LSSLHFVQVGGAKFSEAAARRLPKALGCQLQQVFGMAEGLVNYTRLDDSAELIATTQGRP
CCCEEEEEECCCCHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
ISAHDQLLVVDEQGQPVASGEEGYLLTQGPYTIRGYYRADQHNQRAFNAQGFYITGDKVK
CCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEEEEECCCCCCCEECCCCEEEECCEEE
LSSEGYVIVTGRAKDQINRGGEKIAAEEVENQLLHHPAVHDAALIAISDEYLGERSCAVI
ECCCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCEEEE
VLKPEQSVNTIQLKRFLHQAGLADYKIPDQIQFIDQLPKTSVGKIDKNALRRRFDTLGLA
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LMS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371453; 10952301 [H]