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The map label for this gene is cysS

Identifier: 147673703

GI number: 147673703

Start: 1540378

End: 1541757

Strand: Reverse

Name: cysS

Synonym: VC0395_A1439

Alternate gene names: 147673703

Gene position: 1541757-1540378 (Counterclockwise)

Preceding gene: 147673972

Following gene: 147673791

Centisome position: 50.98

GC content: 46.59

Gene sequence:

>1380_bases
ATGTTGAAGATTTATAACTCACTCACAAGACAGAAAGAGGAATTTAAACCAATCGTTGCCGGCAAAGTGGGCATGTATGT
GTGTGGAGTCACTATTTATGATCTCTGTCATATCGGACATGGGCGTACATTTGTTTCATTTGACGTAGTAGCTCGTTATC
TTCGTTACCTTGGCTACGATCTTACCTTTGTGCGTAATATCACCGACATCGATGACAAGATCATCAAGCGTGCTGCAGAA
AACGCAGAAACGTGCGAATCATTGACAGAACGCTTGATTCAAGAAATGTATGCGGATTTCGATGCCCTGAATATCAAACG
TCCCGATGTTGAACCACGTGCGACGGCTTATATCGAAGAGATCATTGCTCTGGTTGAGCGCCTGATTGAGCGTGGTTTTG
CTTATGTTGCTGATAACGGTGATGTGATGTTTGAAGTGAGCCAATACAGCGAGTACGGTAAACTTTCCAAGCAAGATCTT
GATCAACTCCAAGCGGGAGCACGTGTGGATATCGAAGCGGCAAAACGCAGTCCGCTGGATTTCGTGCTCTGGAAAATGTC
GAAACCGGGTGAACCAACATGGGAATCCCCTTGGGGCTCTGGCCGTCCGGGCTGGCACATCGAGTGTTCAGCGATGAACT
CATCAATTTTGGGTACCCATTTCGATATCCACGGTGGTGGTTCTGACTTGCAATTCCCACATCATGAAAACGAAATTGCG
CAATCTTGCTGCGCCCATGATACCCAGTACGTCAACACTTGGATGCACAGTGGTATGGTGATGGTAGATAAGGAGAAGAT
GTCGAAATCACTCGGTAACTTCTTCACTATCCGCGATGTTTTGGGCCATTATGATGCAGAAACAGTGCGTTATTTCTTGA
TGTCAGGCCACTACAGAAGCCAATTAAACTACAGTGAAGAGAATCTCAATCAAGCACGTGCTTCACTAGAGCGACTCTAT
AACGCACTGCGTGGTTTAGATCGTAGCGTGCCAGCCGCGGGGGGCGAAGAGTACGTGACGCGCTTTACTGCTGCTATGAA
CGATGATTTCAATACGCCAGAAGCGTATTCGGTGCTGTTTGATATGGCTCGTGAAATCAACCGACTCAAGAGTGAAGATA
TGACCAATGCGAGCGCTTTGGGCAGCTTAATGCGTGAGTTGGCAGATGTGATCGGTATTCTTTACCAAGAGCCTGAGGCT
TTCTTCCAAGGTAGTGCGGAAGATGAAGATGCGGCGCAAATTGAAGCTTTAATCAAGCTACGTAATGATTCTCGTGCGAC
GAAAGATTGGGCTAATGCTGACTTGGCGCGTGATAAGTTAAACGAAATGGGAATTGTGCTTGAAGATGGACCTAATGGTA
CAACTTGGCGTCGTAAGTAA

Upstream 100 bases:

>100_bases
AACTTTTTGTGCATTCAAAACATCAAATGGATGACCTAGTAATCTTTGAGTTGTTATACTGTCCGCCATTTATAATTTTG
ATGGTAATCAGATAGAGATC

Downstream 100 bases:

>100_bases
CGAGCAACAAATGAAAGGGTGGGCGTAAGCCCACTCTCTTTTTCTGGCACGTGTAGCCCATCTTTAAACGAACAAGGCAA
GTGAGTTATGTCTGTCATCT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYDLTFVRNITDIDDKIIKRAAE
NAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEEIIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDL
DQLQAGARVDIEAAKRSPLDFVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRSQLNYSEENLNQARASLERLY
NALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLFDMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEA
FFQGSAEDEDAAQIEALIKLRNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK

Sequences:

>Translated_459_residues
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYDLTFVRNITDIDDKIIKRAAE
NAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEEIIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDL
DQLQAGARVDIEAAKRSPLDFVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRSQLNYSEENLNQARASLERLY
NALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLFDMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEA
FFQGSAEDEDAAQIEALIKLRNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK
>Mature_459_residues
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYDLTFVRNITDIDDKIIKRAAE
NAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEEIIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDL
DQLQAGARVDIEAAKRSPLDFVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRSQLNYSEENLNQARASLERLY
NALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLFDMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEA
FFQGSAEDEDAAQIEALIKLRNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=489, Percent_Identity=34.3558282208589, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI10835051, Length=232, Percent_Identity=42.6724137931034, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI303304987, Length=232, Percent_Identity=42.6724137931034, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI21269877, Length=232, Percent_Identity=42.6724137931034, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI62240992, Length=232, Percent_Identity=42.6724137931034, Blast_Score=199, Evalue=7e-51,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=75.0542299349241, Blast_Score=714, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=248, Percent_Identity=40.7258064516129, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17509795, Length=248, Percent_Identity=40.7258064516129, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324082, Length=327, Percent_Identity=34.5565749235474, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI19922218, Length=499, Percent_Identity=34.8697394789579, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922364, Length=260, Percent_Identity=40, Blast_Score=191, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_VIBC3 (A5F763)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F763
- SMR:   A5F763
- STRING:   A5F763
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1439
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   VCHO345073:VC0395_A1439-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52034; Mature: 52034

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYD
CCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LTFVRNITDIDDKIIKRAAENAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEE
HHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHH
IIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDLDQLQAGARVDIEAAKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEEHHHHHHCCCCHHHHHHHHCCCCEEEHHHHCCCCC
FVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHH
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRS
HHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHH
QLNYSEENLNQARASLERLYNALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLF
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
DMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEAFFQGSAEDEDAAQIEALIKL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
RNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK
HCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIYNSLTRQKEEFKPIVAGKVGMYVCGVTIYDLCHIGHGRTFVSFDVVARYLRYLGYD
CCCHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
LTFVRNITDIDDKIIKRAAENAETCESLTERLIQEMYADFDALNIKRPDVEPRATAYIEE
HHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHH
IIALVERLIERGFAYVADNGDVMFEVSQYSEYGKLSKQDLDQLQAGARVDIEAAKRSPLD
HHHHHHHHHHCCCEEEECCCCEEEEEHHHHHHCCCCHHHHHHHHCCCCEEEHHHHCCCCC
FVLWKMSKPGEPTWESPWGSGRPGWHIECSAMNSSILGTHFDIHGGGSDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCHHHHH
QSCCAHDTQYVNTWMHSGMVMVDKEKMSKSLGNFFTIRDVLGHYDAETVRYFLMSGHYRS
HHHHHCCHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCCHHH
QLNYSEENLNQARASLERLYNALRGLDRSVPAAGGEEYVTRFTAAMNDDFNTPEAYSVLF
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHH
DMAREINRLKSEDMTNASALGSLMRELADVIGILYQEPEAFFQGSAEDEDAAQIEALIKL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
RNDSRATKDWANADLARDKLNEMGIVLEDGPNGTTWRRK
HCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA