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The map label for this gene is mltB [H]

Identifier: 147673674

GI number: 147673674

Start: 1662735

End: 1663706

Strand: Reverse

Name: mltB [H]

Synonym: VC0395_A1543

Alternate gene names: 147673674

Gene position: 1663706-1662735 (Counterclockwise)

Preceding gene: 147674473

Following gene: 147674306

Centisome position: 55.02

GC content: 47.53

Gene sequence:

>972_bases
TTGAAAAAATTATTATCGATAGTACTGGGCCTAGCGTTATCGGCACCCGTACTCGCCAATGAAGTCAGTTTTGAACAATA
TGTCGAAAGGCTAAAGCAGCAAGCGCGTGAAGAAGGTATTTCCGAACGTATCCTCAACGAAGCGTTTCGTGATGTGGAAT
ACAAGCCTCGTTCCGTCGTTGCGGATAAAAATCAGCCAGAGAAAAAGTTGACGCTGGATGAATACATCCCGCGCGCTGTA
CCAGAGTGGAAAGTAAAGCAAGCGCAATCTCTGTATGAAAAACACTACACTGAGCTGCAACGCATTGGTAAGCAGTATGG
AGTACAACCGAGATTTATTGTTGCCTTGTGGGGGGTTGAAAGTAATTTCGGTGCGTTCACGGGTAACTTTCGGGTGATTG
ATGCGTTATCGACGCTGGCCTATGAAGGCCGCCGTGAAGAGTTTTTCCGCAAAGAAACCATGGCCGCCTTGCAGATCCTC
GAACAAGGTCACATTGCCCCTGAAGCCATGAAAGGCTCTTGGGCGGGCGCGATGGGGCAGTGTCAATTTATGCCAAGCTC
GTTTTTGAATTTTGCCGCTGATGGCAACGGTGATGGCAAAAAAGACATTTGGGGAACACGTTCAGACGTATTTGCTTCTA
CGGCTAACTATCTCAGCCAATCAGGCTGGGATGATAAGTACACTTGGGGACGCCAAGTGAAAATCCCCAAAGGGTTTGAC
CATGCTCTAGAAGGACGTCAGCCTGAAAAAGGCAAAACGTTACAAGAGTGGAGTAAGCTTGGGGTCACTCGTTATGATGG
TAAACCGCTGCCAGCGCTTTCTGATGATATTAAAGCGTGGCTCATCATGCCTGATGATGAAGCAGGGCGAATTTATCTTG
TGTATAACAACTACAATGTATTGATGAAGTGGAATCGCTCTCATTACTTTGCGTTGGCGGTCAGCCATCTGGCGGATCGG
ATTGCGTTCTAA

Upstream 100 bases:

>100_bases
CTTTTTGCAGTTACCGCCGCCACCAGAAAATTTGCTGGAACAACATAAGGAGCGCAAAGCGCGCCAAACTCCCTGATGAA
GATGGCTCAAGGAGGGCATC

Downstream 100 bases:

>100_bases
CCTGTTGGATTGGCGCAACGGATTGGCAATTAGCGAACAGAGGGCTCTCATTGAGCCCTCTGTTTTTTCTGTTTGTGAGC
AACGGGTTTTTAGGCATTTC

Product: putative lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKKLLSIVLGLALSAPVLANEVSFEQYVERLKQQAREEGISERILNEAFRDVEYKPRSVVADKNQPEKKLTLDEYIPRAV
PEWKVKQAQSLYEKHYTELQRIGKQYGVQPRFIVALWGVESNFGAFTGNFRVIDALSTLAYEGRREEFFRKETMAALQIL
EQGHIAPEAMKGSWAGAMGQCQFMPSSFLNFAADGNGDGKKDIWGTRSDVFASTANYLSQSGWDDKYTWGRQVKIPKGFD
HALEGRQPEKGKTLQEWSKLGVTRYDGKPLPALSDDIKAWLIMPDDEAGRIYLVYNNYNVLMKWNRSHYFALAVSHLADR
IAF

Sequences:

>Translated_323_residues
MKKLLSIVLGLALSAPVLANEVSFEQYVERLKQQAREEGISERILNEAFRDVEYKPRSVVADKNQPEKKLTLDEYIPRAV
PEWKVKQAQSLYEKHYTELQRIGKQYGVQPRFIVALWGVESNFGAFTGNFRVIDALSTLAYEGRREEFFRKETMAALQIL
EQGHIAPEAMKGSWAGAMGQCQFMPSSFLNFAADGNGDGKKDIWGTRSDVFASTANYLSQSGWDDKYTWGRQVKIPKGFD
HALEGRQPEKGKTLQEWSKLGVTRYDGKPLPALSDDIKAWLIMPDDEAGRIYLVYNNYNVLMKWNRSHYFALAVSHLADR
IAF
>Mature_323_residues
MKKLLSIVLGLALSAPVLANEVSFEQYVERLKQQAREEGISERILNEAFRDVEYKPRSVVADKNQPEKKLTLDEYIPRAV
PEWKVKQAQSLYEKHYTELQRIGKQYGVQPRFIVALWGVESNFGAFTGNFRVIDALSTLAYEGRREEFFRKETMAALQIL
EQGHIAPEAMKGSWAGAMGQCQFMPSSFLNFAADGNGDGKKDIWGTRSDVFASTANYLSQSGWDDKYTWGRQVKIPKGFD
HALEGRQPEKGKTLQEWSKLGVTRYDGKPLPALSDDIKAWLIMPDDEAGRIYLVYNNYNVLMKWNRSHYFALAVSHLADR
IAF

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=321, Percent_Identity=27.4143302180685, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36832; Mature: 36832

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLSIVLGLALSAPVLANEVSFEQYVERLKQQAREEGISERILNEAFRDVEYKPRSVV
CHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
ADKNQPEKKLTLDEYIPRAVPEWKVKQAQSLYEKHYTELQRIGKQYGVQPRFIVALWGVE
CCCCCCCHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
SNFGAFTGNFRVIDALSTLAYEGRREEFFRKETMAALQILEQGHIAPEAMKGSWAGAMGQ
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
CQFMPSSFLNFAADGNGDGKKDIWGTRSDVFASTANYLSQSGWDDKYTWGRQVKIPKGFD
EEECHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
HALEGRQPEKGKTLQEWSKLGVTRYDGKPLPALSDDIKAWLIMPDDEAGRIYLVYNNYNV
HHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEE
LMKWNRSHYFALAVSHLADRIAF
EEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLLSIVLGLALSAPVLANEVSFEQYVERLKQQAREEGISERILNEAFRDVEYKPRSVV
CHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEE
ADKNQPEKKLTLDEYIPRAVPEWKVKQAQSLYEKHYTELQRIGKQYGVQPRFIVALWGVE
CCCCCCCHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
SNFGAFTGNFRVIDALSTLAYEGRREEFFRKETMAALQILEQGHIAPEAMKGSWAGAMGQ
CCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
CQFMPSSFLNFAADGNGDGKKDIWGTRSDVFASTANYLSQSGWDDKYTWGRQVKIPKGFD
EEECHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
HALEGRQPEKGKTLQEWSKLGVTRYDGKPLPALSDDIKAWLIMPDDEAGRIYLVYNNYNV
HHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCEE
LMKWNRSHYFALAVSHLADRIAF
EEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]