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The map label for this gene is flgJ [H]

Identifier: 147673638

GI number: 147673638

Start: 1892804

End: 1893742

Strand: Reverse

Name: flgJ [H]

Synonym: VC0395_A1784

Alternate gene names: 147673638

Gene position: 1893742-1892804 (Counterclockwise)

Preceding gene: 147674065

Following gene: 147675062

Centisome position: 62.62

GC content: 48.78

Gene sequence:

>939_bases
ATGATTAATAATTCCAATGACATTGGCTTTATCCAAGATATCGCCGGACTCGACAAGTTACGCCAAAAGGCGGTGAATGG
TGATGAAAATGCCGGACAGTCTGCGCTAACCGCAGCGGCTCGTCAGTTTGAATCGATCTTCACTTCCATGATGCTCAAAT
CCATGCGTGATGCGAATAGTGACTTTAAATCCGATCTGATGAGTAGCCAGAATGAAGATCTTTATCGCCAGATGCTGGAT
GAACAGATGGCGAGTGAGTTCAGTTCATCTGGTTCGCTTGGCTTGGCCGATATGATCGTTGCCCAGCTTTCGACCGGACA
AACGGCGAGCGAGCAAAAAGGGGAGGATGGCTTCCAAGAAGCCATGCGCCGTGTGGAACATGCACGTAAAACGGCCAGTG
AACGCAGCAATGAAGACTTGGTGGCGGCGGTGTATCCGCTGCGTAAAACACAAGCGGTTCAATCGACGCAGTTTGATTCT
CGCCATTCGTTTGTTACTAAGCTCAAACCTTATGCGGATAAAGCGGCACGCATGCTCGGGGTCGATTCTTCCTTGTTGAT
TGCTCAAGCGGCACTGGAAACAGGCTGGGGGCAAAAAATGGTCAAAAATGCCCGTGGCAACAGCAATAACCTCTTTAACA
TCAAAGCCGATCGCAGCTGGCAGGGGGATAAAGTCGCAACCCAAACCTTGGAATATCACAACAATGTTCCAGTGGTGGAG
AAAGCGGCATTTCGCTCTTATGCGAGCTTTGATGAAAGCTTTAACGACTATGTTCGCTTCTTGGAAAACAATCCACGTTA
TACCAATGCGTTGGATCATGGCGGAAACAGTGAACGCTTTATCCACGGTATCCACCGTGCTGGATATGCGACCGACCCGC
AATACGCCGACAAGGTGCTGCGTGTAAAAGCGCAAATCGATCAAATGAATCTGTTATAA

Upstream 100 bases:

>100_bases
GAAGTGGGTGCTGCCCCTTCCGATTTAATGGCGATTTTGCAAGCGCTGAAACAAGCGGGCGCGATTGAAGGCCAACTGAT
CATTATTTAAGGAGCCAGCC

Downstream 100 bases:

>100_bases
ATCGCCCGCCGGAATATTTCCGGCGGCACTCTTTCTCGTCCTCAATCTTTTCAGCTTACCAATCCTAACTTGCTATTTTT
ATTAGAGTAAATCGAATACA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MINNSNDIGFIQDIAGLDKLRQKAVNGDENAGQSALTAAARQFESIFTSMMLKSMRDANSDFKSDLMSSQNEDLYRQMLD
EQMASEFSSSGSLGLADMIVAQLSTGQTASEQKGEDGFQEAMRRVEHARKTASERSNEDLVAAVYPLRKTQAVQSTQFDS
RHSFVTKLKPYADKAARMLGVDSSLLIAQAALETGWGQKMVKNARGNSNNLFNIKADRSWQGDKVATQTLEYHNNVPVVE
KAAFRSYASFDESFNDYVRFLENNPRYTNALDHGGNSERFIHGIHRAGYATDPQYADKVLRVKAQIDQMNLL

Sequences:

>Translated_312_residues
MINNSNDIGFIQDIAGLDKLRQKAVNGDENAGQSALTAAARQFESIFTSMMLKSMRDANSDFKSDLMSSQNEDLYRQMLD
EQMASEFSSSGSLGLADMIVAQLSTGQTASEQKGEDGFQEAMRRVEHARKTASERSNEDLVAAVYPLRKTQAVQSTQFDS
RHSFVTKLKPYADKAARMLGVDSSLLIAQAALETGWGQKMVKNARGNSNNLFNIKADRSWQGDKVATQTLEYHNNVPVVE
KAAFRSYASFDESFNDYVRFLENNPRYTNALDHGGNSERFIHGIHRAGYATDPQYADKVLRVKAQIDQMNLL
>Mature_312_residues
MINNSNDIGFIQDIAGLDKLRQKAVNGDENAGQSALTAAARQFESIFTSMMLKSMRDANSDFKSDLMSSQNEDLYRQMLD
EQMASEFSSSGSLGLADMIVAQLSTGQTASEQKGEDGFQEAMRRVEHARKTASERSNEDLVAAVYPLRKTQAVQSTQFDS
RHSFVTKLKPYADKAARMLGVDSSLLIAQAALETGWGQKMVKNARGNSNNLFNIKADRSWQGDKVATQTLEYHNNVPVVE
KAAFRSYASFDESFNDYVRFLENNPRYTNALDHGGNSERFIHGIHRAGYATDPQYADKVLRVKAQIDQMNLL

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=299, Percent_Identity=35.1170568561873, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 34779; Mature: 34779

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNSNDIGFIQDIAGLDKLRQKAVNGDENAGQSALTAAARQFESIFTSMMLKSMRDANS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DFKSDLMSSQNEDLYRQMLDEQMASEFSSSGSLGLADMIVAQLSTGQTASEQKGEDGFQE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCCHHHHH
AMRRVEHARKTASERSNEDLVAAVYPLRKTQAVQSTQFDSRHSFVTKLKPYADKAARMLG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VDSSLLIAQAALETGWGQKMVKNARGNSNNLFNIKADRSWQGDKVATQTLEYHNNVPVVE
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHH
KAAFRSYASFDESFNDYVRFLENNPRYTNALDHGGNSERFIHGIHRAGYATDPQYADKVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
RVKAQIDQMNLL
HHHHHHHHHCCC
>Mature Secondary Structure
MINNSNDIGFIQDIAGLDKLRQKAVNGDENAGQSALTAAARQFESIFTSMMLKSMRDANS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DFKSDLMSSQNEDLYRQMLDEQMASEFSSSGSLGLADMIVAQLSTGQTASEQKGEDGFQE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCCCHHHHH
AMRRVEHARKTASERSNEDLVAAVYPLRKTQAVQSTQFDSRHSFVTKLKPYADKAARMLG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
VDSSLLIAQAALETGWGQKMVKNARGNSNNLFNIKADRSWQGDKVATQTLEYHNNVPVVE
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHH
KAAFRSYASFDESFNDYVRFLENNPRYTNALDHGGNSERFIHGIHRAGYATDPQYADKVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHH
RVKAQIDQMNLL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9742694; 10952301 [H]