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The map label for this gene is tcpN/toxT

Identifier: 147673596

GI number: 147673596

Start: 390636

End: 391466

Strand: Direct

Name: tcpN/toxT

Synonym: VC0395_A0363

Alternate gene names: 147673596

Gene position: 390636-391466 (Clockwise)

Preceding gene: 147675724

Following gene: 147673195

Centisome position: 12.92

GC content: 28.28

Gene sequence:

>831_bases
ATGATTGGGAAAAAATCTTTTCAAACTAATGTATATAGAATGAGTAAATTTGATACTTACATATTCAATAACTTATATAT
AAACGACTACAAAATGTTCTGGATAGATAGTGGAATTGCAAAGCTTATAGATAAAAATTGCTTGGTTAGTTATGAGATAA
ATTCAAGTTCAATTATCTTACTCAAGAAAAACTCTATTCAGCGATTTTCTTTGACTTCATTATCAGATGAAAATATAAAT
GTTTCTGTAATTACAATAAGTGATTCATTTATACGTTCACTAAAATCTTACATTCTTGGTGATCTCATGATAAGGAATTT
ATATAGTGAAAATAAAGATCTATTACTTTGGAATTGTGAACATAATGATATAGCTGTCCTTTCTGAAGTGGTAAATGGTT
TCAGAGAAATTAATTATTCAGATGAGTTCCTAAAAGTTTTTTTTTCAGGGTTCTTCTCGAAAGTAGAAAAAAAATATAAC
TCTATATTTATTACTGATGATCTTGATGCTATGGAGAAAATTTCATGTTTAGTAAAAAGTGATATTACGCGTAATTGGCG
TTGGGCAGATATTTGTGGTGAATTAAGAACGAATCGGATGATTTTGAAAAAAGAACTAGAGTCTCGAGGAGTAAAGTTTA
GAGAATTAATTAATAGCATTCGAATATCATATTCAATTTCACTAATGAAAACCGGTGAATTCAAAATAAAACAGATTGCA
TATCAGTCTGGGTTTGCTAGCGTTTCATATTTTTCTACAGTATTTAAGTCAACCATGAATGTAGCACCAAGTGAATATTT
ATTTATGTTGACAGGAGTTGCAGAAAAATAA

Upstream 100 bases:

>100_bases
TCTTATGTCTGTTATGTAGTTTTATATTAACTTCGATTTTAAGATAACTTTACGTGGATGGCTCTCTGCGTTTATTTATA
TATCTTCAGAGTAGAACGCA

Downstream 100 bases:

>100_bases
TGGAATACGTTTACTTGATCCTATTTTCGATTGTATCACTGATTTTGGGTAGTTTTAGTAATGTTGTGATTTATCGTCTA
CCAAGAAAAATTTTACTAAA

Product: TCP pilus virulence regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENIN
VSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYN
SIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIA
YQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK

Sequences:

>Translated_276_residues
MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENIN
VSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYN
SIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIA
YQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK
>Mature_276_residues
MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIILLKKNSIQRFSLTSLSDENIN
VSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCEHNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYN
SIFITDDLDAMEKISCLVKSDITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIA
YQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK

Specific function: Probable regulatory protein for the tcp operon

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786776, Length=122, Percent_Identity=29.5081967213115, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789932, Length=222, Percent_Identity=22.972972972973, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789933, Length=172, Percent_Identity=26.7441860465116, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCPN_VIBC3 (A5F384)

Other databases:

- EMBL:   AF325733
- EMBL:   CP000627
- EMBL:   CP001235
- PDB:   3GBG
- PDBsum:   3GBG
- ProteinModelPortal:   A5F384
- STRING:   A5F384
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0363
- eggNOG:   COG2207
- HOGENOM:   HBG264517
- OMA:   INQLPPM
- ProtClustDB:   CLSK793815
- BioCyc:   VCHO345073:VC0395_A0363-MONOMER
- GO:   GO:0005737
- GO:   GO:0009405
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 32055; Mature: 32055

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIIL
CCCCCCHHHHHEEHHHHHEEEECCEEEECEEEEEECCCHHHHHCCCCEEEEEECCCEEEE
LKKNSIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCE
EECCCCCEEEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
HNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYNSIFITDDLDAMEKISCLVKS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHH
DITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIA
HCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHEEEEEEEEEECCCCCEEHHH
YQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK
HHCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCC
>Mature Secondary Structure
MIGKKSFQTNVYRMSKFDTYIFNNLYINDYKMFWIDSGIAKLIDKNCLVSYEINSSSIIL
CCCCCCHHHHHEEHHHHHEEEECCEEEECEEEEEECCCHHHHHCCCCEEEEEECCCEEEE
LKKNSIQRFSLTSLSDENINVSVITISDSFIRSLKSYILGDLMIRNLYSENKDLLLWNCE
EECCCCCEEEECCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
HNDIAVLSEVVNGFREINYSDEFLKVFFSGFFSKVEKKYNSIFITDDLDAMEKISCLVKS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHH
DITRNWRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIA
HCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHEEEEEEEEEECCCCCEEHHH
YQSGFASVSYFSTVFKSTMNVAPSEYLFMLTGVAEK
HHCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11179381