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The map label for this gene is yecK [H]

Identifier: 147673410

GI number: 147673410

Start: 1655912

End: 1657018

Strand: Reverse

Name: yecK [H]

Synonym: VC0395_A1539

Alternate gene names: 147673410

Gene position: 1657018-1655912 (Counterclockwise)

Preceding gene: 147675561

Following gene: 147673939

Centisome position: 54.79

GC content: 49.86

Gene sequence:

>1107_bases
ATGTGTAAGCTTATGTCGATCAAAAAAAGGTATGTTGCACTGCTTGTTGCCATTGGGGTGGCAATTGGCTGGGTCACTTT
AGGTGGCAGTGCTGCAGTGATGCATTACACCTCAAGCACCGAGTTTTGTCTCTCCTGCCATTCTATGGAGATCCCTTTTA
AGGAGTATCAAGGCTCTGTTCACTTTTCCAATGCGAAAGGCATTCGCGCCGAATGTGCCGATTGCCATATTCCGAGCGAT
CCCATCGGTTATTTCGTCACTAAGGTACGAGCGACCAAAGACATCTACCACGAATTTGTAACAGGTAAGATTGATACGCC
TGAGAAGTATGAGGCGCACCGACAAGAGTTAGCGGAGATGGTATGGCAGCAGATGCGCGGTAACGATTCGGCAACGTGTC
GCTCTTGCCATGATTTTGATGCCATGGAGCCGTTTGAGCAGTCACACGAAGCGGTGAAAATGCATGAGTATGCCAAAGCC
AATAACCAAACCTGTATTGATTGCCACAAAGGTGTCGCGCACTTCGCACCTGAAGCGGAGCTTGATAGCAAAGCCTTTGA
AACCTTGATGGCCTTTACCGAAAAAACGGCCGCTGACGCGCAGCGAGTTTACCCCGTCACCACGATTGCGATGGGTGAAT
TGGGTACGATCAACCCGACGACTGCTCTGGATGTTGTGAGTACCAAGGGCACTGAACGTACCGTGCAAATCAACGCTTAC
CAAATGAAAGGCGCAGAACAAGTACTGTATATGGGTGAGGGGCAACGTGCGGTTGTCGCGACTTTAACTGAACAAGGCCA
AAAAGCGCTGCAAGCACAAGACTACAAAACCGATGCTTACGGTAACCAATGGCGCGCGGTGAATTTAACCGCCACCATCA
CATCGCCTGTGGTGGATAGTTTAGAGCCGGTTTGGTCTTATGCCGAACAGCTTGATAACGTTTACTGTGCGACCTGCCAT
GCGAAAATTCCAGCCAGTCACTTTACCGTGAATGCTTGGGGGCCAGTGGCGAAAAGTATGGGAGAGCGGACGGATATTTC
CGCTGAGAATCTGGAGATTCTCACCAAGTTCTTCCAGCACCATGCCAAAGATATGGTTAGCCACTGA

Upstream 100 bases:

>100_bases
ACATCACATAAGCACGCTTTTCGTAGCACGTATATGATGCGGATCAATATCTCATACCCCAAAAGGGGTAGGATGATCGC
ATATTTATAAATGTAAAAGG

Downstream 100 bases:

>100_bases
GTATAAGTAGAGGGATAACGTTATGACTAAAATCACCCGCCGTGGATTTTTAAAAGGCACTGGTATGGCTGCCGGTGCAA
TGGCTTTCACTTCATTTTCA

Product: cytochrome c-type protein YecK

Products: H2O; trimethylamine; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MCKLMSIKKRYVALLVAIGVAIGWVTLGGSAAVMHYTSSTEFCLSCHSMEIPFKEYQGSVHFSNAKGIRAECADCHIPSD
PIGYFVTKVRATKDIYHEFVTGKIDTPEKYEAHRQELAEMVWQQMRGNDSATCRSCHDFDAMEPFEQSHEAVKMHEYAKA
NNQTCIDCHKGVAHFAPEAELDSKAFETLMAFTEKTAADAQRVYPVTTIAMGELGTINPTTALDVVSTKGTERTVQINAY
QMKGAEQVLYMGEGQRAVVATLTEQGQKALQAQDYKTDAYGNQWRAVNLTATITSPVVDSLEPVWSYAEQLDNVYCATCH
AKIPASHFTVNAWGPVAKSMGERTDISAENLEILTKFFQHHAKDMVSH

Sequences:

>Translated_368_residues
MCKLMSIKKRYVALLVAIGVAIGWVTLGGSAAVMHYTSSTEFCLSCHSMEIPFKEYQGSVHFSNAKGIRAECADCHIPSD
PIGYFVTKVRATKDIYHEFVTGKIDTPEKYEAHRQELAEMVWQQMRGNDSATCRSCHDFDAMEPFEQSHEAVKMHEYAKA
NNQTCIDCHKGVAHFAPEAELDSKAFETLMAFTEKTAADAQRVYPVTTIAMGELGTINPTTALDVVSTKGTERTVQINAY
QMKGAEQVLYMGEGQRAVVATLTEQGQKALQAQDYKTDAYGNQWRAVNLTATITSPVVDSLEPVWSYAEQLDNVYCATCH
AKIPASHFTVNAWGPVAKSMGERTDISAENLEILTKFFQHHAKDMVSH
>Mature_368_residues
MCKLMSIKKRYVALLVAIGVAIGWVTLGGSAAVMHYTSSTEFCLSCHSMEIPFKEYQGSVHFSNAKGIRAECADCHIPSD
PIGYFVTKVRATKDIYHEFVTGKIDTPEKYEAHRQELAEMVWQQMRGNDSATCRSCHDFDAMEPFEQSHEAVKMHEYAKA
NNQTCIDCHKGVAHFAPEAELDSKAFETLMAFTEKTAADAQRVYPVTTIAMGELGTINPTTALDVVSTKGTERTVQINAY
QMKGAEQVLYMGEGQRAVVATLTEQGQKALQAQDYKTDAYGNQWRAVNLTATITSPVVDSLEPVWSYAEQLDNVYCATCH
AKIPASHFTVNAWGPVAKSMGERTDISAENLEILTKFFQHHAKDMVSH

Specific function: Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorZ. Required for electron transfer to the TorZ terminal enzyme [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TorC/TorY family [H]

Homologues:

Organism=Escherichia coli, GI1788181, Length=363, Percent_Identity=51.7906336088154, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787230, Length=365, Percent_Identity=37.2602739726027, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1788530, Length=168, Percent_Identity=42.8571428571429, Blast_Score=157, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009154
- InterPro:   IPR011031
- InterPro:   IPR005126 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 40816; Mature: 40816

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKLMSIKKRYVALLVAIGVAIGWVTLGGSAAVMHYTSSTEFCLSCHSMEIPFKEYQGSV
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHEECCCCCCHHHHCCCE
HFSNAKGIRAECADCHIPSDPIGYFVTKVRATKDIYHEFVTGKIDTPEKYEAHRQELAEM
EECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VWQQMRGNDSATCRSCHDFDAMEPFEQSHEAVKMHEYAKANNQTCIDCHKGVAHFAPEAE
HHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHCCCCCC
LDSKAFETLMAFTEKTAADAQRVYPVTTIAMGELGTINPTTALDVVSTKGTERTVQINAY
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHEEEEECCCCCEEEEEEEE
QMKGAEQVLYMGEGQRAVVATLTEQGQKALQAQDYKTDAYGNQWRAVNLTATITSPVVDS
EECCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECHHHHHH
LEPVWSYAEQLDNVYCATCHAKIPASHFTVNAWGPVAKSMGERTDISAENLEILTKFFQH
HHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHH
HAKDMVSH
HHHHHHCC
>Mature Secondary Structure
MCKLMSIKKRYVALLVAIGVAIGWVTLGGSAAVMHYTSSTEFCLSCHSMEIPFKEYQGSV
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHEECCCCCCHHHHCCCE
HFSNAKGIRAECADCHIPSDPIGYFVTKVRATKDIYHEFVTGKIDTPEKYEAHRQELAEM
EECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VWQQMRGNDSATCRSCHDFDAMEPFEQSHEAVKMHEYAKANNQTCIDCHKGVAHFAPEAE
HHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHCCCCCC
LDSKAFETLMAFTEKTAADAQRVYPVTTIAMGELGTINPTTALDVVSTKGTERTVQINAY
CHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHEEEEECCCCCEEEEEEEE
QMKGAEQVLYMGEGQRAVVATLTEQGQKALQAQDYKTDAYGNQWRAVNLTATITSPVVDS
EECCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECHHHHHH
LEPVWSYAEQLDNVYCATCHAKIPASHFTVNAWGPVAKSMGERTDISAENLEILTKFFQH
HHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHH
HAKDMVSH
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine-N-oxide; NADH [C]

Specific reaction: trimethylamine-N-oxide + NADH = H2O + trimethylamine + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]