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The map label for this gene is pspA [H]

Identifier: 147673382

GI number: 147673382

Start: 1364247

End: 1364915

Strand: Reverse

Name: pspA [H]

Synonym: VC0395_A1283

Alternate gene names: 147673382

Gene position: 1364915-1364247 (Counterclockwise)

Preceding gene: 147674406

Following gene: 147675766

Centisome position: 45.14

GC content: 47.38

Gene sequence:

>669_bases
ATGGGTATTTTTTCTCGTTTTGCAGACATCGTAAATTCCAACATCAGCGCGCTACTGGATAAAGCAGAAGATCCAGAAAA
AATGATCCGCCTGATTATTCAGGAGATGGAAGATACGTTAGTGGAAGTGCGTACCAATTCAGCCAAAGCAATGGCAGATA
AAAAAGAGCTGGCGCGTAAAGTGAGGTCGATTGAACAGCAGTTAGAGGATTGGCAGAACAAAGCGACGCTGGCGCTGACT
AAACAACGTGAAGATTTGGCGCGCGCGGCACTGATCGAGAAGCAGAAACTGCAACATGTACTGAAAGGCTTGCACACAGA
GCAGACGCTTGTCGAAGAAACCATTGAAAAGCTGACAGGCGAAATTGGTAAGCTCGAAACCAAAATCACTGAAACCCGTG
CGAAACAGCAAGCGTTAGCGATTCGTAGCCAAGCGGCGAGTCATCGTCGCGATGTGCAGCGTCATTTGCACGCGGGTCGT
AGCGAAGAAGCGATGGCAAAGTTTGAGCAGTTCTCGCGTAAAGTCGATGAGCTGGAAGCGGAAGCCGATCTTTATGCACA
ATCAGGCCAAGCGCGCTCTTTGGAGCAAGAATTTGCTGAGCTACAAGCACAAGATGAGATTGAAAAAGAGCTCAACAAGC
TTAAAGATCAGATGAAATCAGACCAATAA

Upstream 100 bases:

>100_bases
AAAGTTGGCACGTAATTTGGATTTATCTTTGCAACCAACGCACAGCATGGGGCTGCAGCGGTAATCTCAGTGTTTGGCAC
TGAAGGATAGGAGAAACGTC

Downstream 100 bases:

>100_bases
TTGGGAGTAGACTATGTCCTCATTTTTTATCGCTGTGCCATTGATCGTTTTTTGTATCTTTGTTGCACCGCTGTGGCTGA
TCCTCCACTACCGGAGTAAA

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVRSIEQQLEDWQNKATLALT
KQREDLARAALIEKQKLQHVLKGLHTEQTLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGR
SEEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKDQMKSDQ

Sequences:

>Translated_222_residues
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVRSIEQQLEDWQNKATLALT
KQREDLARAALIEKQKLQHVLKGLHTEQTLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGR
SEEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKDQMKSDQ
>Mature_221_residues
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARKVRSIEQQLEDWQNKATLALTK
QREDLARAALIEKQKLQHVLKGLHTEQTLVEETIEKLTGEIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRS
EEAMAKFEQFSRKVDELEAEADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKDQMKSDQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=221, Percent_Identity=57.9185520361991, Blast_Score=251, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25409; Mature: 25278

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARK
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VRSIEQQLEDWQNKATLALTKQREDLARAALIEKQKLQHVLKGLHTEQTLVEETIEKLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
EIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRSEEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKDQMKSDQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIFSRFADIVNSNISALLDKAEDPEKMIRLIIQEMEDTLVEVRTNSAKAMADKKELARK
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VRSIEQQLEDWQNKATLALTKQREDLARAALIEKQKLQHVLKGLHTEQTLVEETIEKLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
EIGKLETKITETRAKQQALAIRSQAASHRRDVQRHLHAGRSEEAMAKFEQFSRKVDELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EADLYAQSGQARSLEQEFAELQAQDEIEKELNKLKDQMKSDQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]