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The map label for this gene is pfkA [H]

Identifier: 147673340

GI number: 147673340

Start: 2410656

End: 2411651

Strand: Reverse

Name: pfkA [H]

Synonym: VC0395_A2261

Alternate gene names: 147673340

Gene position: 2411651-2410656 (Counterclockwise)

Preceding gene: 147673451

Following gene: 147675567

Centisome position: 79.75

GC content: 49.3

Gene sequence:

>996_bases
TTGATATTCGATTCGCAAAGTTCGAGGGTGAGCATGATTAAAAAAATCGGTGTTTTGACGAGTGGTGGCGATGCCCCAGG
TATGAATGCAGCTATTCGCGGCGTGGTGCGTACAGCACTTGGCGCAGGTTTAGAAGTGTTTGGTATTTACGACGGCTACC
AAGGTCTGTACGAAGATCGTATTAAGCAGCTAGACCGTTCAAGCGTGTCTGACGTGATCAACCGTGGCGGTACTTTTTTA
GGCTCTGCACGCTTCCCACAATTTCGTGAAGTTGAAGTGCGCGAAAAAGCGATTGAAAACCTGAAAAAGCACGGCATTGA
AGCGCTGGTTGTGATTGGTGGTGACGGCTCTTACATGGGCGCCAAAAAACTGACCGAAATGGGTTTCCCTTGTATCGGTC
TGCCAGGCACGATTGATAACGATATCGCGGGCACCGACTACACCATCGGTTATTTGACTGCCCTGAACACCGTTATTGAA
GCGATTGACCGTCTGCGCGATACCTCTTCTTCACACCAACGTATCTCTATCGTAGAAATCATGGGTCGTCACTGTGGTGA
CTTGACGCTGACCTCTGCGATTGCGGGTGGCTGTGAATACATCATCACACCGGAAACGGGTCTGAATATGGAGCAGCTGA
TTTCCAATATCAAAGATGGTATTGCGAAAGGCAAAAAGCACGCGATCATTGCGCTGACTGAGCTGATGATGGATGCCAAC
AAACTGGCCAAAGAGATTGAATCGGCGACAGGCCGTGAAACTCGCGCAACTGTATTAGGCCATATTCAACGTGGTGGTAA
ACCTACCGCATTTGACCGTGTACTGGCATCGCGCATGGGTAACTACGCGGTGCATCTATTGATGGAAGGTCACGGTGGTC
GTTGTGTGGGTATCCAGAAAGAGCAACTGGTTCACCACGACATCATTGATGCGATTGAAAACATGAAGCGTCCTGTACGC
ACTGACCTGTACAAAGTCGCTGAAGAGCTGTTCTAA

Upstream 100 bases:

>100_bases
TAGTGCAAATAACAGTAGTATTCCTAATCGGGAAAGGTAACATTACGCACAGTTTTTGAATTTTTCTGTCTGGTTCCTTA
CAAGCCAGACGGCGTAGAAA

Downstream 100 bases:

>100_bases
GTTCGCAGCACTTTGTATTCAGAGACAAAAAGACCGCCAAGGCGGTCTTTTTTATACGGGTTTGTTTATATGCGGATTAG
AGAATGATGTCGCGCACTTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIFDSQSSRVSMIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDRIKQLDRSSVSDVINRGGTFL
GSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMGAKKLTEMGFPCIGLPGTIDNDIAGTDYTIGYLTALNTVIE
AIDRLRDTSSSHQRISIVEIMGRHCGDLTLTSAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDAN
KLAKEIESATGRETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQKEQLVHHDIIDAIENMKRPVR
TDLYKVAEELF

Sequences:

>Translated_331_residues
MIFDSQSSRVSMIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDRIKQLDRSSVSDVINRGGTFL
GSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMGAKKLTEMGFPCIGLPGTIDNDIAGTDYTIGYLTALNTVIE
AIDRLRDTSSSHQRISIVEIMGRHCGDLTLTSAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDAN
KLAKEIESATGRETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQKEQLVHHDIIDAIENMKRPVR
TDLYKVAEELF
>Mature_331_residues
MIFDSQSSRVSMIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDRIKQLDRSSVSDVINRGGTFL
GSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMGAKKLTEMGFPCIGLPGTIDNDIAGTDYTIGYLTALNTVIE
AIDRLRDTSSSHQRISIVEIMGRHCGDLTLTSAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDAN
KLAKEIESATGRETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQKEQLVHHDIIDAIENMKRPVR
TDLYKVAEELF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=43.0868167202572, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI48762920, Length=313, Percent_Identity=43.7699680511182, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=42.9936305732484, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=42.9936305732484, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=42.9936305732484, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=42.9936305732484, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=75.9375, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=39.871382636656, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=38.6363636363636, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=31.0344827586207, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=36.8421052631579, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323861, Length=344, Percent_Identity=33.1395348837209, Blast_Score=179, Evalue=6e-46,
Organism=Drosophila melanogaster, GI28573326, Length=317, Percent_Identity=42.5867507886435, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=42.443729903537, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=42.3076923076923, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35909; Mature: 35909

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDSQSSRVSMIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDR
CCCCCCCHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
IKQLDRSSVSDVINRGGTFLGSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMG
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
AKKLTEMGFPCIGLPGTIDNDIAGTDYTIGYLTALNTVIEAIDRLRDTSSSHQRISIVEI
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
MGRHCGDLTLTSAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDAN
HHHHCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KLAKEIESATGRETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEECCCH
EQLVHHDIIDAIENMKRPVRTDLYKVAEELF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIFDSQSSRVSMIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDR
CCCCCCCHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
IKQLDRSSVSDVINRGGTFLGSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMG
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
AKKLTEMGFPCIGLPGTIDNDIAGTDYTIGYLTALNTVIEAIDRLRDTSSSHQRISIVEI
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
MGRHCGDLTLTSAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDAN
HHHHCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KLAKEIESATGRETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEECCCH
EQLVHHDIIDAIENMKRPVRTDLYKVAEELF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]