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The map label for this gene is dapF

Identifier: 147673333

GI number: 147673333

Start: 2547954

End: 2548784

Strand: Reverse

Name: dapF

Synonym: VC0395_A2393

Alternate gene names: 147673333

Gene position: 2548784-2547954 (Counterclockwise)

Preceding gene: 147674363

Following gene: 147673737

Centisome position: 84.28

GC content: 52.23

Gene sequence:

>831_bases
ATGCATTTCCATTTTTCTAAAATGCACGGTTTGGGCAACGACTTTATGGTCGTGGACTGTATTACCCAAAACGTCTTCTT
CTCTCCGGAATTGATCCGCCGTCTGGCGGATCGCCATACGGGAGTCGGCTTTGATCAATTGTTGGTGGTTGAAGCGCCTT
ACGATCCAGAATCGGATTTCCATTACCGCATTTTCAATGCCGATGGCAGCGAAGTGGAGCAGTGTGGCAATGGCGCGCGC
TGTTTTGCCCGTTTTGTACGCATGAAAGGGTTAACCAACAAGTACACCATCCATGTCAGTACCAAGAAAGGCAAAATGGT
ACTCAATGTGGAAGAGGAAGATCTGATCACCGTGAACATGGGTGTGCCGGAGTTTGAGCCGAACAAGATCCCATTTCGCG
CCAAACAGAGCGAAAAAACCTACATTCTGCGTGTCGGTGAACACACCTTGTTTTGCGGCGCGGTGAGTATGGGCAACCCG
CATGTAGTGACTGTGGTGGACGATATCCGCACTGCTGCGGTAGAAACCTTAGGGCCGCTTTTGGAATCGCACGAGCGTTT
CCCTGAGCGCGTCAACGCCGGTTTTATGCAGGTCGTGAGCCGTGATGAGATCAATTTGCGCGTGTATGAACGTGGTGCGG
GCGAAACTCAAGCTTGTGGCAGTGGCGCGTGCGCCGCGGTTGCGGTAGGCATTTTGCAAGGTTTACTGGATGAGCAAGTT
CGTGTCCATCTTCCCGGTGGTGAGCTGGAAATCCACTGGCAAGGCCCCGGCAAACCGCTGTATATGACAGGCCCGGCTAC
TCATATCTACGATGGCCAAATCTCTTGCTAA

Upstream 100 bases:

>100_bases
TCATGGTTGATGGCAATAAAACCTATTTGGTGCGTCAGCGTGAAGAGCTTAGCAGCTTGTGGGCGTTAGAATCGGTATTA
CCGGAGTAAAGTTATCTTCT

Downstream 100 bases:

>100_bases
TTGAGGTTCAATTTTGTCTCAGGTAACGGCGGATGCTCTGACTGCCCAAGTGGTGGCAGAGTATCTTTATGAACATCCCG
ATTTTTTTCAGCATCATCCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNP
HVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQV
RVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC

Sequences:

>Translated_276_residues
MHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNP
HVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQV
RVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
>Mature_276_residues
MHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDFHYRIFNADGSEVEQCGNGAR
CFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNMGVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNP
HVVTVVDDIRTAAVETLGPLLESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQV
RVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=272, Percent_Identity=77.9411764705882, Blast_Score=449, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_VIBC3 (A5F4I6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4I6
- SMR:   A5F4I6
- STRING:   A5F4I6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2393
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   VCHO345073:VC0395_A2393-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30651; Mature: 30651

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDF
CCEECHHHCCCCCCEEEEEEECCCCEECHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCEEEEEC
GVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNPHVVTVVDDIRTAAVETLGPL
CCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHH
LESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQV
HHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
RVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
EEEECCCEEEEEEECCCCCEEEECCCCEEECCEECC
>Mature Secondary Structure
MHFHFSKMHGLGNDFMVVDCITQNVFFSPELIRRLADRHTGVGFDQLLVVEAPYDPESDF
CCEECHHHCCCCCCEEEEEEECCCCEECHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
HYRIFNADGSEVEQCGNGARCFARFVRMKGLTNKYTIHVSTKKGKMVLNVEEEDLITVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEECCCCEEEEEC
GVPEFEPNKIPFRAKQSEKTYILRVGEHTLFCGAVSMGNPHVVTVVDDIRTAAVETLGPL
CCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEEHHHHHHHHHHHHHH
LESHERFPERVNAGFMQVVSRDEINLRVYERGAGETQACGSGACAAVAVGILQGLLDEQV
HHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
RVHLPGGELEIHWQGPGKPLYMTGPATHIYDGQISC
EEEECCCEEEEEEECCCCCEEEECCCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA