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The map label for this gene is grpE

Identifier: 147673276

GI number: 147673276

Start: 410298

End: 410900

Strand: Direct

Name: grpE

Synonym: VC0395_A0381

Alternate gene names: 147673276

Gene position: 410298-410900 (Clockwise)

Preceding gene: 147675728

Following gene: 147674898

Centisome position: 13.57

GC content: 49.42

Gene sequence:

>603_bases
ATGAGCAACGAAGAGATCAAGAACAAAGACGAGCAGCTGCAACAAGACGCGGTTGAGACTGAAGCTGAAGTTGTAGGAAC
AGATGCCGATATCGACTGGAACCAAGCCGCTGACGAAATCGATGAGAAAGAAGCGAAAATTGCTCAGTTAGAAGCGGCGC
TGCTTGTGAGCGAAGAGCGCGTGAAAGAGCAGCAAGACAGCGTACTGCGTGCCCGTGCGGAAGTCGAAAACATGCGTCGC
CGTAGCGAGCAGGAAGTGGATAAAGCACGTAAATTTGCGCTGAGCCGCTTTGCTGAAGAGCTACTGCCAGTGATTGATAA
CCTTGAGCGTGCGATTCAAGCCGCCGATGGTGAAGTTGAAGCGATCAAACCTCTGCTGGAAGGTGTAGAACTGACGCACA
AAACTTTCGTTGATACGATTGCGAAGTTCGGCTTGAAAGAGATTAACCCGCACGGTGAAGTGTTTAACCCTGAATTCCAC
CAAGCCATGTCGATTCAAGAAAGCGCTGAGCATGAGCCAAACACGGTGATGTTTGTGATGCAAAAAGGCTATGAACTGAA
TGGTCGTGTATTACGCCCTGCTATGGTGATGGTGTCTAAGTAA

Upstream 100 bases:

>100_bases
ATAATAAGGGCAAGTTGTGCTTTTATGCGAATTTTTGTGTTGGGAAATCGACGATTAGACGATTTCTCCTGACCAGTATG
AAGTGAATTTTGGAGATATC

Downstream 100 bases:

>100_bases
TTCCTAGACCCTGAGCGACAGAAGAAGCGAAGCCGATTGGCTTCGCTTTTTTATGTTCTAAAACCGATTTATGCGCAAAA
ACACAATAAATCTGAATTTT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSNEEIKNKDEQLQQDAVETEAEVVGTDADIDWNQAADEIDEKEAKIAQLEAALLVSEERVKEQQDSVLRARAEVENMRR
RSEQEVDKARKFALSRFAEELLPVIDNLERAIQAADGEVEAIKPLLEGVELTHKTFVDTIAKFGLKEINPHGEVFNPEFH
QAMSIQESAEHEPNTVMFVMQKGYELNGRVLRPAMVMVSK

Sequences:

>Translated_200_residues
MSNEEIKNKDEQLQQDAVETEAEVVGTDADIDWNQAADEIDEKEAKIAQLEAALLVSEERVKEQQDSVLRARAEVENMRR
RSEQEVDKARKFALSRFAEELLPVIDNLERAIQAADGEVEAIKPLLEGVELTHKTFVDTIAKFGLKEINPHGEVFNPEFH
QAMSIQESAEHEPNTVMFVMQKGYELNGRVLRPAMVMVSK
>Mature_199_residues
SNEEIKNKDEQLQQDAVETEAEVVGTDADIDWNQAADEIDEKEAKIAQLEAALLVSEERVKEQQDSVLRARAEVENMRRR
SEQEVDKARKFALSRFAEELLPVIDNLERAIQAADGEVEAIKPLLEGVELTHKTFVDTIAKFGLKEINPHGEVFNPEFHQ
AMSIQESAEHEPNTVMFVMQKGYELNGRVLRPAMVMVSK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=148, Percent_Identity=37.8378378378378, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=31.7567567567568, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788967, Length=165, Percent_Identity=44.2424242424242, Blast_Score=147, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=36.8794326241135, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=167, Percent_Identity=34.1317365269461, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653432, Length=164, Percent_Identity=33.5365853658537, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_VIBC3 (A5F369)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F369
- SMR:   A5F369
- STRING:   A5F369
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0381
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14150
- BioCyc:   VCHO345073:VC0395_A0381-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22660; Mature: 22529

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEEIKNKDEQLQQDAVETEAEVVGTDADIDWNQAADEIDEKEAKIAQLEAALLVSEER
CCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEQQDSVLRARAEVENMRRRSEQEVDKARKFALSRFAEELLPVIDNLERAIQAADGEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AIKPLLEGVELTHKTFVDTIAKFGLKEINPHGEVFNPEFHQAMSIQESAEHEPNTVMFVM
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
QKGYELNGRVLRPAMVMVSK
ECCCCCCCEEEHHHHHHHCC
>Mature Secondary Structure 
SNEEIKNKDEQLQQDAVETEAEVVGTDADIDWNQAADEIDEKEAKIAQLEAALLVSEER
CCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEQQDSVLRARAEVENMRRRSEQEVDKARKFALSRFAEELLPVIDNLERAIQAADGEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AIKPLLEGVELTHKTFVDTIAKFGLKEINPHGEVFNPEFHQAMSIQESAEHEPNTVMFVM
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
QKGYELNGRVLRPAMVMVSK
ECCCCCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA