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The map label for this gene is rlmF

Identifier: 147673215

GI number: 147673215

Start: 1281479

End: 1281700

Strand: Reverse

Name: rlmF

Synonym: VC0395_A1219

Alternate gene names: 147673215

Gene position: 1281700-1281479 (Counterclockwise)

Preceding gene: 147675585

Following gene: 147675636

Centisome position: 42.38

GC content: 45.5

Gene sequence:

>222_bases
ATGGCGGTAGAAAGCCAGCAGTTCGCGCAGCAAGTGCTTTGGTTTAGCACTTTGATTTCTAAAGGTGATAACGTTCGTGG
CATGAAAAAACAGCTGGAAAAACTCGGGGCTCAGTCGATACATGTAATAGAGATGGCACAAGGGCAGAAGATAAGTCGCT
TTATTGCTTGGAGTTTTCAAAATGCAGAGCAGCGCAAGCTGTGGTGGCAGGCAAAATGTTAA

Upstream 100 bases:

>100_bases
GCAGCAGTTAGAGTTATTGAGAGACTAACAGCGTCGTGGCCCCCTTTTAGGGGGCTTATATTAAAACCGCCTTCTAAGAA
GGCGGATTTTTATTGGTAAA

Downstream 100 bases:

>100_bases
GCTTTAAACAAGTTACTGAACATGAAAAGTAAAAAGCCAATCGCTCCTAAATAAAAATCCAGGAGGGGCAAGATGAGAAT
TGAAGACGTTCCACTCTACG

Product: methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 73; Mature: 72

Protein sequence:

>73_residues
MAVESQQFAQQVLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC

Sequences:

>Translated_73_residues
MAVESQQFAQQVLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC
>Mature_72_residues
AVESQQFAQQVLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Escherichia coli, GI87081786, Length=66, Percent_Identity=51.5151515151515, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_VIBC3 (A5F7R5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F7R5
- SMR:   A5F7R5
- STRING:   A5F7R5
- eggNOG:   COG3129
- ProtClustDB:   PRK11727
- BioCyc:   VCHO345073:VC0395_A1219-MONOMER
- BioCyc:   VCHO345073:VC0395_A1221-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 8498; Mature: 8367

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVESQQFAQQVLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQ
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCC
NAEQRKLWWQAKC
CCHHHHCEECCCC
>Mature Secondary Structure 
AVESQQFAQQVLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCC
NAEQRKLWWQAKC
CCHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA