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The map label for this gene is luxO

Identifier: 147673171

GI number: 147673171

Start: 580984

End: 582351

Strand: Direct

Name: luxO

Synonym: VC0395_A0541

Alternate gene names: 147673171

Gene position: 580984-582351 (Clockwise)

Preceding gene: 147673403

Following gene: 147674934

Centisome position: 19.21

GC content: 49.78

Gene sequence:

>1368_bases
ATGGTAGAAGACACGGCGTCGGTGGCGGCGCTGTATCGTTCTTACCTCACACCGCTGGATATTGATATCAATATCGTGGG
TACCGGACGCGATGCCATCGAGAGTATTGGCCGTCGCGAGCCTGACTTAATCCTGCTGGATTTACGTTTGCCAGATATGA
CGGGGATGGACGTACTCTATGCAGTGAAAGAGAAATCACCGGATGTGCCTATCGTGTTTATGACCGCTCATGGTTCGATT
GATACGGCGGTTGAAGCCATGCGTCATGGTGCGCAAGACTTTTTGATCAAGCCGTGTGAAGCGGACCGACTGCGGGTCAC
AGTGAATAATGCGATTCGCAAAGCCTCGAAACTCAAAAACGATGTTGATAATAAAAATCAAAACTATCAAGGCTTTATTG
GTAGCAGTCAAACCATGCAGGCGGTGTACCGCACCATTGACTCTGCGGCGAGCAGCAAAGCCAGTATTTTTATTACCGGC
GAAAGTGGTACAGGTAAAGAAGTGTGCGCGGAAGCGATTCATGCCGCGAGCAAGCGTGGAGATAAGCCATTTATCGCCAT
CAACTGTGCGGCCATTCCGAAAGATCTGATTGAAAGTGAGTTGTTTGGTCACGTCAAAGGGGCTTTTACTGGGGCGGCGA
CTGAGCGTCAAGGCGCGGCAGAAGCGGCTGATGGGGGAACCCTCTTTTTGGATGAATTGTGCGAAATGGATCTGGATCTG
CAGACCAAACTTCTGCGCTTTATTCAGACAGGGACATTCCAAAAAGTCGGCTCTTCCAAAATGAAAAGCGTGGATGTGCG
TTTTGTGTGTGCAACGAACCGCGATCCGTGGAAGGAAGTGCAAGAAGGACGCTTTCGTGAAGACCTGTACTACCGCTTGT
ATGTGATCCCGCTGCATTTACCGCCATTGCGCGCGCGTGGTGATGATGTGATCGAGATTGCTTATTCACTGCTTGGCTTT
ATGTCCAAGGAAGAGGGCAAAGATTTTGTCCGTTTGTCGGCTGAAGTGGTGGAGCGTTTTCGCCAATACGAGTGGCCGGG
CAACGTGCGTCAATTGCAAAACGTTTTGCGCAACGTGGTTGTGCTCAATGAAGGTCGTGAAATCACCCTAGATATGCTGC
CTCCTCCTCTTAATCAAATGTCCGCGCCGATCAATCGGGCTTTACCGCTTGCGCATGAGAATAAAGTATCCGTGCATGAG
ATTTTTCCGCTGTGGATGACCGAAAAACAAGCTATTGAACAAGCCATCGAAGCGTGTGATGGCAACATTCCCCGTGCCGC
AACCTATCTGGATGTCAGCCCGTCAACCATCTATCGCAAGCTGCAAACTTGGAATGAAAAAGTGAAAGAAAAAGAGAAGG
AACGGTAA

Upstream 100 bases:

>100_bases
CAAAATGCAATTCCAAATGCAATTATTACAGCAAAATGCAAAATAATATGGCTAGGCTATGCAACATAATCAATCTTTGC
AGAAAACAAAATATGTCCTC

Downstream 100 bases:

>100_bases
GCGATGAGAGAATGGATCAACCAAAGCAAAATCGACTTACTGGCGAAGGAGATCGGTGAAGAAAACGTGCCGATCTTGGT
CAACATTTTTCTTGGTGAAC

Product: LuxO repressor protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSI
DTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITG
ESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGF
MSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHE
IFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER

Sequences:

>Translated_455_residues
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSI
DTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITG
ESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGF
MSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHE
IFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER
>Mature_455_residues
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSI
DTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITG
ESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGF
MSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHE
IFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER

Specific function: Involved in the regulation of different processes depending on the cell density. Acts together with sigma-54 to repress, perhaps indirectly, some genes

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=39.8706896551724, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=39.2290249433107, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=38.4105960264901, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788905, Length=336, Percent_Identity=41.6666666666667, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI87082117, Length=371, Percent_Identity=39.0835579514825, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=41.6403785488959, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=314, Percent_Identity=42.3566878980892, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789087, Length=293, Percent_Identity=40.6143344709898, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786524, Length=360, Percent_Identity=36.9444444444444, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=263, Percent_Identity=39.5437262357414, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081872, Length=314, Percent_Identity=36.624203821656, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=36.5384615384615, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=27.5510204081633, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUXO_VIBCH (Q9KT84)

Other databases:

- EMBL:   AE003852
- PIR:   C82250
- RefSeq:   NP_230666.1
- ProteinModelPortal:   Q9KT84
- SMR:   Q9KT84
- GeneID:   2614291
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1021
- TIGR:   VC_1021
- HOGENOM:   HBG740884
- OMA:   SYLNPLG
- ProtClustDB:   CLSK874182
- GO:   GO:0005622
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 50840; Mature: 50840

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLY
CCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHE
AVKEKSPDVPIVFMTAHGSIDTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKN
EECCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHEECCCCCCCEEEEHHHHHHHHHHHHH
DVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITGESGTGKEVCAEAIHAASKRG
HHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
DKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
CCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCH
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHL
HHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEEC
PPLRARGDDVIEIAYSLLGFMSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVV
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
VLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHEIFPLWMTEKQAIEQAIEACD
EECCCCEEEEECCCCCHHHHCCCHHHCCCCCCCCCCCHHHCCHHHCCHHHHHHHHHHHHC
GNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLY
CCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHE
AVKEKSPDVPIVFMTAHGSIDTAVEAMRHGAQDFLIKPCEADRLRVTVNNAIRKASKLKN
EECCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHEECCCCCCCEEEEHHHHHHHHHHHHH
DVDNKNQNYQGFIGSSQTMQAVYRTIDSAASSKASIFITGESGTGKEVCAEAIHAASKRG
HHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
DKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDL
CCCEEEEEEHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHCCCCCH
QTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHL
HHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEEC
PPLRARGDDVIEIAYSLLGFMSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVV
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHE
VLNEGREITLDMLPPPLNQMSAPINRALPLAHENKVSVHEIFPLWMTEKQAIEQAIEACD
EECCCCEEEEECCCCCHHHHCCCHHHCCCCCCCCCCCHHHCCHHHCCHHHHHHHHHHHHC
GNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 9748465