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The map label for this gene is ksgA

Identifier: 147673129

GI number: 147673129

Start: 3013793

End: 3014608

Strand: Reverse

Name: ksgA

Synonym: VC0395_A2861

Alternate gene names: 147673129

Gene position: 3014608-3013793 (Counterclockwise)

Preceding gene: 147674027

Following gene: 147675104

Centisome position: 99.69

GC content: 48.53

Gene sequence:

>816_bases
ATGAGAAATGATGTCCACTTAGGGCACAAAGCGCGTAAACGCTTTGGTCAGAACTTCCTCAATGATCCTTACATCATTGA
TGGTATTGTGTCGGCAATTAATCCCAAACCGGGGCAAAACTTGGTTGAAATCGGCCCAGGGTTAGGCGCCATCACAGAAC
CGGTAGGACGTGAAGTGGATAAGTTCACTGTCATTGAGCTTGACCGCGATCTGGCAGAACGCCTGCGTAATCATCCAGAA
TTAGCCAGCAAGTTGACCATTCATGAAGGCGATGCGATGCGTTTCGACTTCAAGCAGTTGGTGAAACCCAATAACAAACT
TCGCGTGTTTGGTAACTTACCTTACAACATTTCAACGCCGCTGATGTTCCACTTGTTTGAATTCCACCGTGATATTCAAG
ACATGCACTTCATGCTGCAAAAAGAGGTGGTGAACCGATTAGCCGCAGGTCCGGGAACCAAGGCCTACGGTCGACTAACC
GTGATGGCGCAATACTACTGTAAAGTGGTTCCGGTACTAGAAGTGCCACCTAGCGCATTTGTGCCACCACCAAAAGTGGA
TTCGGCCGTGGTACGTTTGGTGCCTTATGAAGATCTCCCTCATCCAGCAACCAGCTTAGAGTGGTTAGATCGCGTGGTAC
GTGAAGGCTTTAACCAACGCCGTAAAACGGTGCGTAACTGTTATAAAGGTTTGGCTGAACCGGAAACACTGGAAACTCTG
GGCATCAACCCAGGTATGCGCCCAGAAAATCTGACCTTGGCTCAATTTGTGGCATTAGCGAACTGGCTTGATGCCACACA
CAAAACTCACGCATAA

Upstream 100 bases:

>100_bases
CTGGCTGGAACGGGTCAAGCCGATGCGGGAAGCTTTTGGACTGCATTAGCTTACGCAATTGAATTAGTTGATAAAAAAGC
GCAATAACTTAGAGAAAACT

Downstream 100 bases:

>100_bases
TCGATAAGGACGACATTCAGTCGTCCTTTGTTTATCTGCCAATATCTGACTACTAAGCTGAGGAGTCGCTCATGGATGTT
TCGCTGCCCTGTATCAAAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPE
LASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETL
GINPGMRPENLTLAQFVALANWLDATHKTHA

Sequences:

>Translated_271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPE
LASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETL
GINPGMRPENLTLAQFVALANWLDATHKTHA
>Mature_271_residues
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVDKFTVIELDRDLAERLRNHPE
LASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTPLMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLT
VMAQYYCKVVPVLEVPPSAFVPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETL
GINPGMRPENLTLAQFVALANWLDATHKTHA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=32.6241134751773, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI7657198, Length=251, Percent_Identity=32.6693227091633, Blast_Score=114, Evalue=6e-26,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=66.2878787878788, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=35.1598173515982, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=29.5918367346939, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=233, Percent_Identity=30.4721030042918, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=34.2960288808664, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21358017, Length=241, Percent_Identity=34.0248962655602, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_VIBC3 (A5F8N2)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F8N2
- SMR:   A5F8N2
- STRING:   A5F8N2
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2861
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   VCHO345073:VC0395_A2861-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30689; Mature: 30689

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCC
KFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTP
CEEEEECCHHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHCCCCCCEEEEECCCCCCCHH
LMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCC
VPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETL
CCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GINPGMRPENLTLAQFVALANWLDATHKTHA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNDVHLGHKARKRFGQNFLNDPYIIDGIVSAINPKPGQNLVEIGPGLGAITEPVGREVD
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCC
KFTVIELDRDLAERLRNHPELASKLTIHEGDAMRFDFKQLVKPNNKLRVFGNLPYNISTP
CEEEEECCHHHHHHHHCCHHHHHHEEECCCCCCCCCHHHHCCCCCCEEEEECCCCCCCHH
LMFHLFEFHRDIQDMHFMLQKEVVNRLAAGPGTKAYGRLTVMAQYYCKVVPVLEVPPSAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCC
VPPPKVDSAVVRLVPYEDLPHPATSLEWLDRVVREGFNQRRKTVRNCYKGLAEPETLETL
CCCCCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GINPGMRPENLTLAQFVALANWLDATHKTHA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA