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The map label for this gene is fadL-2 [H]

Identifier: 147673128

GI number: 147673128

Start: 602256

End: 603542

Strand: Reverse

Name: fadL-2 [H]

Synonym: VC0395_A0561

Alternate gene names: 147673128

Gene position: 603542-602256 (Counterclockwise)

Preceding gene: 147673825

Following gene: 147675389

Centisome position: 19.96

GC content: 45.84

Gene sequence:

>1287_bases
ATGAACAACATGCGTCTGTTTAAAAAATCCCTGCTTGCCGTGACGGTAACGCTAGCCACACAACAAGCGTTTGCAGCAGG
TTTCCAGCTCAACGCACAATCTGCTACCGGCCTAGGTCGCGCTTTTGCGGGTGATGCCGTTATCGCCGATAACGCAGCCG
TAATGTCTCGCAACCCAGCGGCGATGGCACTGTTTGATGAGATGGCGCTCTCTCTTGGTTTTGAAACCATTACCACCATG
ATTGACGTTAAAGATGTCTCTTATCGAGGTGTTCTCGGTAACTCAGTTTCTGTTCAAGACACGGATGACGTAGGTGGTAC
TTCCGTTGCACCTAATATTCATTTGATCGTTCCAGTCAATGAACAGTTTGCTTGGGGGGTTAATGCTTATTCAAACTTTG
GTACAAAAACTGAGTTTTCTGATTCTTATCCAGCCTCTGAATACGGTGGCTTAACTGACGTTAAAAGCTTTAACTTAGGC
CTAGCAGGTTCTTATCGCGTCAACAAAGAATGGAGTTTCGGTGCTGGTTTAGACCTGATTTATGGGCAAGGCACCATGAA
ACGTATTGCCGGAACCAGTTTCCCAGCAAGTACAATTGTGGTGAACCCGACGACCGTTATCCCAGCTCGAGGCACATCAC
TACTCAACGTCGATAAAGCGGATGGTTGGGCAGTAGGTTTTAACTTGGGTACAGTTTACGAAATGGATGAGAATAACCGC
TTTGGTTTGTCTTACCGCTATAGCCCAGAATTTAAAGCTAAAGACGATTACGGACAAGAGATCACGCTACCACTACCGGA
TATCGCTGAGTTCTCTGGCTTCCACAAAATTGAGAATACTCAGTTTGCCGTACACTATAGCGTACAGTGGATTGGCTGGA
GTGCTTTCGACAAAATTGAGTTCCGCAACCTTGTAACGTCTCGCTCTTCTGTTGGCGCTCTAACCAAAGGCAGCTACGAT
AAGATGTATGAGTGGCAAGATGGCTGGCACTACTCTATCGGTGGCACATACTACTTAAATAGTGATTGGACACTGCGTGC
AGGCTATATGTACGATACTAGCGCACAAGATTCACGTACTTCTATCTCTGTTCCTGATTCAGATCGCCAGTGGTTATCAG
CAGGTTTTACCTATAACATCGATGACAAATCAAACGTAGACTTTGGATTTACTTACTTAATGGGTAAAGATGTCGCAGTT
TCCGAAAATGATGCGGGTTCTAAGCTAACCGCAACAACCCACGCAGACGCAATCCTGTTGGGCTTACAATACAGCCGTAC
GTTTTAA

Upstream 100 bases:

>100_bases
ACTAGCGTCTAGGATCAGCCACCACTGAGCAATTGCTCAAATAAAGCAAAAGCTCATACAACAACGCCCATAAGAGGCGA
AACCAAAAAGGAAAAGTCTA

Downstream 100 bases:

>100_bases
TTAAAACGTCACAACATATCATTACTAAGGGGCTGATTTATCAGCCCCTTTCTTTTGCTGACTGCCTAGTTTTGCTTAAT
GTCAGCGTACAAGCGTGCGA

Product: long-chain fatty acid transport protein

Products: NA

Alternate protein names: Outer membrane fadL protein; Outer membrane flp protein [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKDVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF

Sequences:

>Translated_428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKDVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF
>Mature_428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKDVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF

Specific function: Involved in translocation of long-chain fatty acids across the outer membrane. FadL may form a specific channel [H]

COG id: COG2067

COG function: function code I; Long-chain fatty acid transport protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ompP1/fadL family [H]

Homologues:

Organism=Escherichia coli, GI145693163, Length=440, Percent_Identity=36.1363636363636, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR005017 [H]

Pfam domain/function: PF03349 Toluene_X [H]

EC number: NA

Molecular weight: Translated: 46798; Mature: 46798

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPA
CCCHHHHHHCEEEEEEEEEHHHHHHCCEEEECCCCCCCCHHHCCCEEEECCCEEECCCCC
AMALFDEMALSLGFETITTMIDVKDVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVN
HHHHHHHHHHHHCHHHHEEEEEEECCEECCCCCCCEEECCCCCCCCCEECCCEEEEEECC
EQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLGLAGSYRVNKEWSFGAGLDLI
CCEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCEEECCCCCCCCCEEEE
YGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
ECCCHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCE
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIE
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHCCCEEECCCEEEEEEEEEEEEECHHCHHH
FRNLVTSRSSVGALTKGSYDKMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRT
HHHHHHCCCCCCCCCCCCCHHCEECCCCEEEECCEEEEECCCEEEEEEEEECCCCCCCCC
SISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAVSENDAGSKLTATTHADAILL
EEECCCCCHHHHHCCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCEEEE
GLQYSRTF
EEEECCCC
>Mature Secondary Structure
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPA
CCCHHHHHHCEEEEEEEEEHHHHHHCCEEEECCCCCCCCHHHCCCEEEECCCEEECCCCC
AMALFDEMALSLGFETITTMIDVKDVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVN
HHHHHHHHHHHHCHHHHEEEEEEECCEECCCCCCCEEECCCCCCCCCEECCCEEEEEECC
EQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLGLAGSYRVNKEWSFGAGLDLI
CCEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCEEECCCCCCCCCEEEE
YGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
ECCCHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCE
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIE
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHCCCEEECCCEEEEEEEEEEEEECHHCHHH
FRNLVTSRSSVGALTKGSYDKMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRT
HHHHHHCCCCCCCCCCCCCHHCEECCCCEEEECCEEEEECCCEEEEEEEEECCCCCCCCC
SISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAVSENDAGSKLTATTHADAILL
EEECCCCCHHHHHCCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCEEEE
GLQYSRTF
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]