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The map label for this gene is rnfE

Identifier: 147673085

GI number: 147673085

Start: 571762

End: 572454

Strand: Reverse

Name: rnfE

Synonym: VC0395_A0533

Alternate gene names: 147673085

Gene position: 572454-571762 (Counterclockwise)

Preceding gene: 147675771

Following gene: 147674958

Centisome position: 18.93

GC content: 51.23

Gene sequence:

>693_bases
ATGAGTGAAAACCGAACTCTGATGTTAAATGGCATGTGGAACAACAACCCAGCCTTAGTGCAATTACTGGGATTGTGTCC
GCTGCTCGCCGTTTCCTCCACCGTGACCAATGCCCTCGGATTAGGCATTGCCACGCTTCTTGTTCTCGTCGGCTCGAATG
TCACCGTCTCTCTCGTGCGTGATTATGTGCCGAAAGAAGTCCGAATTCCGGTGTTCGTGATGATCATCGCTTCGCTGGTG
ACTTGCGTACAGTTACTGATGAACGCCTACGCTTACGGGCTTTATCTCTCGCTCGGCATTTTTATTCCGCTGATTGTGAC
CAACTGCATTATCATAGGTCGTGCTGAAGCGTTTGCTTCAAAAAATGATGTGCTCCCAGCCGCGTTAGACGGATTTTGGA
TGGGATTGGGGATGACCTCAGTACTGGTTGTGCTCGGATCACTGCGCGAAATCATCGGCAATGGCACGCTGTTCGATGGC
GCGGACCTCCTACTCGGAGAATGGGCTAAAGTTCTACGCATCGAAGTGTTTCACTTTGACAGCGCCTTTTTGCTGGCACT
GCTGCCCCCCGGTGCCTTCATCGGCGTTGGCTTTTTGATCGCCGCTAAAAGCGTGATTGATAAACAGATTGCCGCTCGCC
AACCAAAACAACAAAAGCAAGCCATAGAGCGTGCACGCGTGACCAATGTCTAA

Upstream 100 bases:

>100_bases
CCTCGCGCTGTCGTACTGGCGGTCAAAAAAGCCGTGGAGTATGTCAACCAACATCAACAACAGCTGCACAATCAGCCGAA
TCCGTGTGAGGGTCAATAAC

Downstream 100 bases:

>100_bases
CCAGATCAAGGAAGCCAACCCGTCTAATGAATAACGCGAAACGGATTGAAATTTTAGAACGGCTGAGGGCTAACAACCCA
AAACCCGAAACCGAATTAAA

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDG
ADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV

Sequences:

>Translated_230_residues
MSENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDG
ADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV
>Mature_229_residues
SENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLVT
CVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGA
DLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=210, Percent_Identity=67.1428571428571, Blast_Score=274, Evalue=4e-75,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=32.0512820512821, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_VIBC3 (A5F2S7)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- STRING:   A5F2S7
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0533
- eggNOG:   COG4660
- HOGENOM:   HBG624915
- OMA:   ENPVTKM
- ProtClustDB:   PRK12405
- BioCyc:   VCHO345073:VC0395_A0533-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 24696; Mature: 24565

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17d52888)-; HASH(0x1865b698)-; HASH(0x17f3ba28)-; HASH(0x182f9848)-; HASH(0x17c805a8)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVR
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYVPKEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCC
KNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGADLLLGEWAKVLRIEVFHFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEECC
SAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
>Mature Secondary Structure 
SENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVR
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYVPKEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCC
KNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGADLLLGEWAKVLRIEVFHFD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEECC
SAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA