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The map label for this gene is fixJ [H]

Identifier: 146343750

GI number: 146343750

Start: 7298350

End: 7298985

Strand: Reverse

Name: fixJ [H]

Synonym: BRADO6997

Alternate gene names: 146343750

Gene position: 7298985-7298350 (Counterclockwise)

Preceding gene: 146343751

Following gene: 146343747

Centisome position: 97.89

GC content: 61.95

Gene sequence:

>636_bases
ATGGCCGAACAAACAACTTCCCGCGGTGAGATTTTTGTCGTCGATGACGACCCAGCCGTGCGCGACACCCTCTCGATGGT
CTTGTCGGCGGGTGGGTATGAGGTGATCTGCTTTGCGGATGGCGCGGCGCTCCTTGCAGTGGCCCGAAGCCGAACACCTT
CCTGCATCCTGCTCGACGTGCACATCCCGGGAAAGTCGGGGCTCGACATCCTCAGGGAACTTCATGGCGAGGACTATCCG
GCGCCGATCTTCATGATCTCGGGCCAAGGCGACATCCCGATGGCCGTCAACGCCATCAAGAACGGCGCGCTCGACTTCAT
CGAGAAGCCGTTTCGCGGCAGTGAAATCGTGGCTCGCTTGAACGAAGCGATCGGCGCGTTCGCGCGCCGTCAGAAGGAGA
ACGCGTCGCCGAAGATCGGCTCGCTGCATTTTCCCGGTCGCGAGCCACTGACCAGGCGCGAGCGCGAGGTGCTGGAACAA
TTCACGGCGGGGGCCTCGAACAAGGAGGCCGGACGCACCCTCGGCATCAGTCCACGGACGATCGAGGACCATCGCGCCAA
CATCATGAAGAAGCTCGGCGCACGCAATGCAGCAGATCTGATTCGTATCGTGATGACGGCGTCGCGCGCCAATTGA

Upstream 100 bases:

>100_bases
GCTTAAGCCGTACAAATACGGGTGCCCAAACTACCTGGCGAGTATGGTATCATAAGACGCGCTTTCATTGTTTCTTCTCT
CTTTGCAAGAGCAATCGCCC

Downstream 100 bases:

>100_bases
AAGGCTGCCGGGTCGCGCACGCGCAGCCTGGCGCGTACTGACGTGCCGGCGTGACGCCGGGTCCAAGCGTCGGTGCTCGA
GTGTCGGTGTTTAGGCGTCG

Product: two-component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAEQTTSRGEIFVVDDDPAVRDTLSMVLSAGGYEVICFADGAALLAVARSRTPSCILLDVHIPGKSGLDILRELHGEDYP
APIFMISGQGDIPMAVNAIKNGALDFIEKPFRGSEIVARLNEAIGAFARRQKENASPKIGSLHFPGREPLTRREREVLEQ
FTAGASNKEAGRTLGISPRTIEDHRANIMKKLGARNAADLIRIVMTASRAN

Sequences:

>Translated_211_residues
MAEQTTSRGEIFVVDDDPAVRDTLSMVLSAGGYEVICFADGAALLAVARSRTPSCILLDVHIPGKSGLDILRELHGEDYP
APIFMISGQGDIPMAVNAIKNGALDFIEKPFRGSEIVARLNEAIGAFARRQKENASPKIGSLHFPGREPLTRREREVLEQ
FTAGASNKEAGRTLGISPRTIEDHRANIMKKLGARNAADLIRIVMTASRAN
>Mature_210_residues
AEQTTSRGEIFVVDDDPAVRDTLSMVLSAGGYEVICFADGAALLAVARSRTPSCILLDVHIPGKSGLDILRELHGEDYPA
PIFMISGQGDIPMAVNAIKNGALDFIEKPFRGSEIVARLNEAIGAFARRQKENASPKIGSLHFPGREPLTRREREVLEQF
TAGASNKEAGRTLGISPRTIEDHRANIMKKLGARNAADLIRIVMTASRAN

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=33.6065573770492, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788550, Length=138, Percent_Identity=26.8115942028986, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1790860, Length=147, Percent_Identity=29.2517006802721, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=27.7272727272727, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=30.9734513274336, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790863, Length=109, Percent_Identity=31.1926605504587, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22836; Mature: 22705

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQTTSRGEIFVVDDDPAVRDTLSMVLSAGGYEVICFADGAALLAVARSRTPSCILLDV
CCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEE
HIPGKSGLDILRELHGEDYPAPIFMISGQGDIPMAVNAIKNGALDFIEKPFRGSEIVARL
ECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHH
NEAIGAFARRQKENASPKIGSLHFPGREPLTRREREVLEQFTAGASNKEAGRTLGISPRT
HHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCC
IEDHRANIMKKLGARNAADLIRIVMTASRAN
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEQTTSRGEIFVVDDDPAVRDTLSMVLSAGGYEVICFADGAALLAVARSRTPSCILLDV
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEEEE
HIPGKSGLDILRELHGEDYPAPIFMISGQGDIPMAVNAIKNGALDFIEKPFRGSEIVARL
ECCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHH
NEAIGAFARRQKENASPKIGSLHFPGREPLTRREREVLEQFTAGASNKEAGRTLGISPRT
HHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCC
IEDHRANIMKKLGARNAADLIRIVMTASRAN
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]