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The map label for this gene is otsA [H]

Identifier: 146343706

GI number: 146343706

Start: 7235841

End: 7237172

Strand: Reverse

Name: otsA [H]

Synonym: BRADO6952

Alternate gene names: 146343706

Gene position: 7237172-7235841 (Counterclockwise)

Preceding gene: 146343707

Following gene: 146343699

Centisome position: 97.06

GC content: 65.17

Gene sequence:

>1332_bases
GTGCGTGATGGTGCGCAGGCGGAGCCGATCGCGCAGATCGAACAGCTCGGCCAAGGCGCACTGGCGCTGCTGGACCTGCC
CGCCGCGCATTACGCCGGCTACTACGAAGGCTTCGCCAATTCGGCGCTGTGGCCGGCGCTGCATTCGCGCAGCGATCTCA
TCAGAGTCTCGCAAGACGACTACAATTCCTATCGCGAGGTCAATTCGTTCGTCGCCCGCGCTCTGCTCAAGTTCCAGAAG
CAGGACACGCTGTTCTGGATTCAGGACTATCATTTCCTGTCGTTGGGCGCGGAGCTGCGGACGCTCGGCGTCAAGAGCCC
GATCGGCTTCTTCCTGCACACGCCGTGGCCGGCGCGCAGCGTGATCGACAGCGTGCCGAATCACCGCGACCTCGTCACCG
CGATGCTCGCTTACGATCTGATCGGTTTCCAGACCGAAGACGATCGGGAGAATTTCGCCGCCTGCGTGCGGGCCGATCTC
GGTCTGCAGGTGCGCAACGGCGTCGTCACCTCGCGGCACGGCCGCACGCGACTTGCGGTGTTTCCGATCGGCATCGACGC
CGACAAGTTCGCGCAGCAGGCGGCCAAGGCGGCGTCGCATCCGGACGTGTCGCGGCTGCGCCGGAGCCTGCATGGGGAGA
AGCTCGCGATCGGCGTCGACCGGCTCGACTACTCCAAGGGCCTCGTCAACCGCATCAACGCGTTCGACCGGATGTGGAGT
GTCCATCCGACCCTGCTGCGCGCGGCGTCGCTGCTGCAGATCGCGACGCCGTCGCGCGAATCCATCGAGGCTTATGGCAA
TCTGCAGAGCGAGGTTGCGCGTCTCGTCAGCGAGGTCAACGGCCGCCACGGCGAGGTCGATTGGACGCCGATCCGCTATC
TCAACAAAGGCTACGGCCAAGGCGTGCTCGCCGGTCTCTATCGCAGCGCGCAGGTCGGTGTCGTCACGCCGCTGCATGAC
GGCATGAACCTCGTTGCCAAGGAATATGTCGCGGCGCAAAGCCCGGTCGATCCCGGCGTGCTGGTGCTGTCGAAATTCGC
CGGCGCCGCCAATGAACTCGACGCGGCACTGCTGGTCAACCCGCACGATATCGACGGCATGGCGCGTACGATCGCGATGG
CACTGTCGATGCCGCTGAATGAGCGGCGCATGCGTCACCAGATCCTGATGACGAAGCTGCGCCGCCATTCGATCCAGCAA
TGGTTCGCGGACTTCCTCGATGCGCTGCAGGACGACGGCGCCCACGAGCCGGTCGAGGTCGAACCCCTGCCTGCGGCGTC
GGCGGGATTGCTCGGCAGCCGCTTCATGTCGTCGACCGCCCGTTATCACTGA

Upstream 100 bases:

>100_bases
GCGTGGCAAGGCCAACGAGCCAATGACCGGCGGACTCGCGGCAGCCCTGCTGCCCGTTGTTGAGAATTCCGGAGCAGTTT
GGGTGGGTTCCTCGGGCCGT

Downstream 100 bases:

>100_bases
GGCCAGGACGGGCCGCGGAATGGCAGGTCGGCCCGGCGCGATCGGCTCAATGGCAATACGACACGCCGTCCAGCACCGCG
CAGCGCAGCTTGTTCGGCGA

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRDGAQAEPIAQIEQLGQGALALLDLPAAHYAGYYEGFANSALWPALHSRSDLIRVSQDDYNSYREVNSFVARALLKFQK
QDTLFWIQDYHFLSLGAELRTLGVKSPIGFFLHTPWPARSVIDSVPNHRDLVTAMLAYDLIGFQTEDDRENFAACVRADL
GLQVRNGVVTSRHGRTRLAVFPIGIDADKFAQQAAKAASHPDVSRLRRSLHGEKLAIGVDRLDYSKGLVNRINAFDRMWS
VHPTLLRAASLLQIATPSRESIEAYGNLQSEVARLVSEVNGRHGEVDWTPIRYLNKGYGQGVLAGLYRSAQVGVVTPLHD
GMNLVAKEYVAAQSPVDPGVLVLSKFAGAANELDAALLVNPHDIDGMARTIAMALSMPLNERRMRHQILMTKLRRHSIQQ
WFADFLDALQDDGAHEPVEVEPLPAASAGLLGSRFMSSTARYH

Sequences:

>Translated_443_residues
MRDGAQAEPIAQIEQLGQGALALLDLPAAHYAGYYEGFANSALWPALHSRSDLIRVSQDDYNSYREVNSFVARALLKFQK
QDTLFWIQDYHFLSLGAELRTLGVKSPIGFFLHTPWPARSVIDSVPNHRDLVTAMLAYDLIGFQTEDDRENFAACVRADL
GLQVRNGVVTSRHGRTRLAVFPIGIDADKFAQQAAKAASHPDVSRLRRSLHGEKLAIGVDRLDYSKGLVNRINAFDRMWS
VHPTLLRAASLLQIATPSRESIEAYGNLQSEVARLVSEVNGRHGEVDWTPIRYLNKGYGQGVLAGLYRSAQVGVVTPLHD
GMNLVAKEYVAAQSPVDPGVLVLSKFAGAANELDAALLVNPHDIDGMARTIAMALSMPLNERRMRHQILMTKLRRHSIQQ
WFADFLDALQDDGAHEPVEVEPLPAASAGLLGSRFMSSTARYH
>Mature_443_residues
MRDGAQAEPIAQIEQLGQGALALLDLPAAHYAGYYEGFANSALWPALHSRSDLIRVSQDDYNSYREVNSFVARALLKFQK
QDTLFWIQDYHFLSLGAELRTLGVKSPIGFFLHTPWPARSVIDSVPNHRDLVTAMLAYDLIGFQTEDDRENFAACVRADL
GLQVRNGVVTSRHGRTRLAVFPIGIDADKFAQQAAKAASHPDVSRLRRSLHGEKLAIGVDRLDYSKGLVNRINAFDRMWS
VHPTLLRAASLLQIATPSRESIEAYGNLQSEVARLVSEVNGRHGEVDWTPIRYLNKGYGQGVLAGLYRSAQVGVVTPLHD
GMNLVAKEYVAAQSPVDPGVLVLSKFAGAANELDAALLVNPHDIDGMARTIAMALSMPLNERRMRHQILMTKLRRHSIQQ
WFADFLDALQDDGAHEPVEVEPLPAASAGLLGSRFMSSTARYH

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=408, Percent_Identity=38.7254901960784, Blast_Score=298, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI115533558, Length=413, Percent_Identity=28.5714285714286, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI115533560, Length=413, Percent_Identity=28.5714285714286, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=441, Percent_Identity=27.2108843537415, Blast_Score=151, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319602, Length=385, Percent_Identity=33.2467532467532, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6320279, Length=431, Percent_Identity=24.8259860788863, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6323537, Length=388, Percent_Identity=24.7422680412371, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323917, Length=387, Percent_Identity=24.8062015503876, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19920676, Length=402, Percent_Identity=37.5621890547264, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 48968; Mature: 48968

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDGAQAEPIAQIEQLGQGALALLDLPAAHYAGYYEGFANSALWPALHSRSDLIRVSQDD
CCCCCCCCHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCHH
YNSYREVNSFVARALLKFQKQDTLFWIQDYHFLSLGAELRTLGVKSPIGFFLHTPWPARS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCHHHHHHCCCCCCCCEEECCCCHHH
VIDSVPNHRDLVTAMLAYDLIGFQTEDDRENFAACVRADLGLQVRNGVVTSRHGRTRLAV
HHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCCEEEEE
FPIGIDADKFAQQAAKAASHPDVSRLRRSLHGEKLAIGVDRLDYSKGLVNRINAFDRMWS
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
VHPTLLRAASLLQIATPSRESIEAYGNLQSEVARLVSEVNGRHGEVDWTPIRYLNKGYGQ
CCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
GVLAGLYRSAQVGVVTPLHDGMNLVAKEYVAAQSPVDPGVLVLSKFAGAANELDAALLVN
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEC
PHDIDGMARTIAMALSMPLNERRMRHQILMTKLRRHSIQQWFADFLDALQDDGAHEPVEV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
EPLPAASAGLLGSRFMSSTARYH
CCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRDGAQAEPIAQIEQLGQGALALLDLPAAHYAGYYEGFANSALWPALHSRSDLIRVSQDD
CCCCCCCCHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCHH
YNSYREVNSFVARALLKFQKQDTLFWIQDYHFLSLGAELRTLGVKSPIGFFLHTPWPARS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHCHHHHHHCCCCCCCCEEECCCCHHH
VIDSVPNHRDLVTAMLAYDLIGFQTEDDRENFAACVRADLGLQVRNGVVTSRHGRTRLAV
HHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCCEEEEE
FPIGIDADKFAQQAAKAASHPDVSRLRRSLHGEKLAIGVDRLDYSKGLVNRINAFDRMWS
EEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
VHPTLLRAASLLQIATPSRESIEAYGNLQSEVARLVSEVNGRHGEVDWTPIRYLNKGYGQ
CCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
GVLAGLYRSAQVGVVTPLHDGMNLVAKEYVAAQSPVDPGVLVLSKFAGAANELDAALLVN
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEC
PHDIDGMARTIAMALSMPLNERRMRHQILMTKLRRHSIQQWFADFLDALQDDGAHEPVEV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
EPLPAASAGLLGSRFMSSTARYH
CCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]