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The map label for this gene is pcaR [H]

Identifier: 146343429

GI number: 146343429

Start: 6914624

End: 6915511

Strand: Direct

Name: pcaR [H]

Synonym: BRADO6658

Alternate gene names: 146343429

Gene position: 6914624-6915511 (Clockwise)

Preceding gene: 146343425

Following gene: 146343431

Centisome position: 92.73

GC content: 63.96

Gene sequence:

>888_bases
GTGAAAAAGCTACAACCGAAGCCAAGGGGCAAACGGACAGCAGCTTCACTGCGGCCGAGAACAACAACAGCGGATCGTGT
CAGGCCGATGCCAAAACTAAAGCGGACTGATGAGGAGAAGAAGCAGGCGCGTGGCGACGGGCCTGAGTTCGTCGAAGCAC
TCGATCGAGGGCTTCGCGTGATACAGGCCTTCAGCCGCGAAGGCCGGCCGATGACGTTGAGCAAGGCCGCCGAACTCACA
GGTCTCGCGCGTGCCACGGTGCGCCGCATCCTGCTGACGCTGAAGGCATCGGGCTTCGTTCATGGCGACGATCGGCTGTT
CTCGCTGACGCCGCGGGTGCTGTTGTTAGCATCCAGCTACCTCGCATCGAACCAGATCAACACCGTGGTACAGCCGTTGC
TGGATGTCGCGGCGACGGAGGCCCGCGAGGTCTGTTCTCTCGCGGTTCTCGATGGCGATCAGGTCGTGTTCATCGCGCGC
GCCACGCCGACGCGGATGTTCTCCAGTGGGCTCGAGATCGGCTACAGGCTGCCGGCCTTCTGCACCTCCGTGGGGCGGGT
GCTGCTCAGTCGCATGCCGGACGATCAGTTGCGGCGGGCCATCGAGCAGACGCCGCTCACGCCGATGACGCCGCAAACGA
CCACCGACCAGGAAGCGCTGATCGCCGCGATATCGACCGTGCGCAGCGACGGTTATTCGGTGGTCGACGGCGAGGCCGAG
CCCGGGTTCCGCTCGATCTCGGTGCCGATCCGCCGCTACGACGGCAGCATCGTTGCGGCCGCGAATATTGGCGGGCACGT
CGACCGGATTACGATCGACGAGATGAAAGAGCGCTTTCTGCCGTTGCTGAAGCAGCTTGCGACGACCGTGCAGCCCCTGC
TGGTCTGA

Upstream 100 bases:

>100_bases
CGCAAGTCGGGCGTTGACGTCACGCGATCATCTAATATTGTTCGCTAGACGAACACATGTTCGCCAAGATTAGATTCGCT
TTAACTGGGGAGAAGCGCAA

Downstream 100 bases:

>100_bases
TTTCACTGATCCTAAGCGGCACCGGCTTCACTGAAGCGGTGCCGGCCGGCGCGTCGGCCCTGCGCACGCCCCTGCCATCT
CCTCCAGCTTAGCTCTGCCA

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKKLQPKPRGKRTAASLRPRTTTADRVRPMPKLKRTDEEKKQARGDGPEFVEALDRGLRVIQAFSREGRPMTLSKAAELT
GLARATVRRILLTLKASGFVHGDDRLFSLTPRVLLLASSYLASNQINTVVQPLLDVAATEAREVCSLAVLDGDQVVFIAR
ATPTRMFSSGLEIGYRLPAFCTSVGRVLLSRMPDDQLRRAIEQTPLTPMTPQTTTDQEALIAAISTVRSDGYSVVDGEAE
PGFRSISVPIRRYDGSIVAAANIGGHVDRITIDEMKERFLPLLKQLATTVQPLLV

Sequences:

>Translated_295_residues
MKKLQPKPRGKRTAASLRPRTTTADRVRPMPKLKRTDEEKKQARGDGPEFVEALDRGLRVIQAFSREGRPMTLSKAAELT
GLARATVRRILLTLKASGFVHGDDRLFSLTPRVLLLASSYLASNQINTVVQPLLDVAATEAREVCSLAVLDGDQVVFIAR
ATPTRMFSSGLEIGYRLPAFCTSVGRVLLSRMPDDQLRRAIEQTPLTPMTPQTTTDQEALIAAISTVRSDGYSVVDGEAE
PGFRSISVPIRRYDGSIVAAANIGGHVDRITIDEMKERFLPLLKQLATTVQPLLV
>Mature_295_residues
MKKLQPKPRGKRTAASLRPRTTTADRVRPMPKLKRTDEEKKQARGDGPEFVEALDRGLRVIQAFSREGRPMTLSKAAELT
GLARATVRRILLTLKASGFVHGDDRLFSLTPRVLLLASSYLASNQINTVVQPLLDVAATEAREVCSLAVLDGDQVVFIAR
ATPTRMFSSGLEIGYRLPAFCTSVGRVLLSRMPDDQLRRAIEQTPLTPMTPQTTTDQEALIAAISTVRSDGYSVVDGEAE
PGFRSISVPIRRYDGSIVAAANIGGHVDRITIDEMKERFLPLLKQLATTVQPLLV

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=228, Percent_Identity=23.2456140350877, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082359, Length=231, Percent_Identity=26.4069264069264, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786715, Length=224, Percent_Identity=27.6785714285714, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788131, Length=221, Percent_Identity=27.6018099547511, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789999, Length=269, Percent_Identity=25.2788104089219, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI145693096, Length=225, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788581, Length=231, Percent_Identity=22.5108225108225, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32375; Mature: 32375

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLQPKPRGKRTAASLRPRTTTADRVRPMPKLKRTDEEKKQARGDGPEFVEALDRGLRV
CCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHH
IQAFSREGRPMTLSKAAELTGLARATVRRILLTLKASGFVHGDDRLFSLTPRVLLLASSY
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHHHHH
LASNQINTVVQPLLDVAATEAREVCSLAVLDGDQVVFIARATPTRMFSSGLEIGYRLPAF
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCHHHHHHHCCCCCCCHHHH
CTSVGRVLLSRMPDDQLRRAIEQTPLTPMTPQTTTDQEALIAAISTVRSDGYSVVDGEAE
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCC
PGFRSISVPIRRYDGSIVAAANIGGHVDRITIDEMKERFLPLLKQLATTVQPLLV
CCCEEEECCCEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLQPKPRGKRTAASLRPRTTTADRVRPMPKLKRTDEEKKQARGDGPEFVEALDRGLRV
CCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHH
IQAFSREGRPMTLSKAAELTGLARATVRRILLTLKASGFVHGDDRLFSLTPRVLLLASSY
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEHHHHHHHHHHHH
LASNQINTVVQPLLDVAATEAREVCSLAVLDGDQVVFIARATPTRMFSSGLEIGYRLPAF
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCHHHHHHHCCCCCCCHHHH
CTSVGRVLLSRMPDDQLRRAIEQTPLTPMTPQTTTDQEALIAAISTVRSDGYSVVDGEAE
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCC
PGFRSISVPIRRYDGSIVAAANIGGHVDRITIDEMKERFLPLLKQLATTVQPLLV
CCCEEEECCCEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]