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The map label for this gene is ynfL [H]

Identifier: 146343424

GI number: 146343424

Start: 6908966

End: 6909886

Strand: Direct

Name: ynfL [H]

Synonym: BRADO6653

Alternate gene names: 146343424

Gene position: 6908966-6909886 (Clockwise)

Preceding gene: 146343423

Following gene: 146343425

Centisome position: 92.66

GC content: 67.64

Gene sequence:

>921_bases
ATGGAGATGCATCAGGTCCGTTACTTCCTCACCGTCGCCAAGCTTCTGAATTTTTCCCGTGCCGCCGATGAATGCAACGT
GACGCAGCCGTCGCTGTCGCGCGCCATCAAGCAGCTGGAGGCGGAGATTGGCGGCGAGCTGTTCGTGCGAGGACCACCGT
CGGCACGACTGACCGCGCTCGGCCACGCCGTGCATCCTGCGCTGAAGCAGTGCTACGACGCCGCGCTCGGCGCGCGGGCG
GTGGCGATCTCGATGAGGCAGCGACCGCAGACCAGCCTTCGCCTGGCCCTCACCGAATCGATCGATCTGGCTCCCGTCAT
TCCTCACATCCAGCGCATCCGCAACCGCTTCGAGCATCTCGACCTGACCGTTCAGCGCGGCGACGGCGCCAAGGCCGCGG
CGATGCTGCAAGACGGGGACGTCGAGCTGGTCATCGCGGCGGATCTGCACAAGGCGGGCTCTCATCTCCACCAGCTGCCG
CTGTTCACCGAGGGATTCGACCTCGTGGTCCACACGCAGCACCGGCTCGCAGGCCGGCCGCTGGTTCCAGCCATGGAGCT
TTGCCGGGAGCCGTTGCTGGTCGGAGCCTATTGCGAGCATACGCCGGGCATCCTCGAAGGGCTGAGACGCCACGGCGTCG
GTGCGATCCGTGCGGGCGAGGTCCACGCCGAGCGCGATCTGCTCGGGCTCGTCGCAGCCGGCCTCGGCGTCGCGCTCCTG
CCACGCAGCGCGCAGCTGCCGGCGGGATTGCGCCGCATCGCCATTGCCGGTCTTGCGATCGAGCGAACGGTGAGCCTCTA
CAGCCTGGCAGGACGTCCCTGGAGCAGGATCGGCGCCGCCGTCATCAACCAGATCAGACGATCCCGGACCGATAGGCCGC
AGCGACGCGCACCGCTCGACGCTGCCGGTGAAAACCCGTAG

Upstream 100 bases:

>100_bases
CACGAATAGCCCGGCGCGTGCTGCAGCGTCACCCCGGCTTCGGCCGGGCTCCCCAGCGCGCCGGCCGGCGCCTTCGTTCC
CGCTCATCAGGGTCGCGCCG

Downstream 100 bases:

>100_bases
AGACGGTGCCCGGCAATAGGCGCGGCGAGCCGCGCACCGCATCGGGAGTTTTCGCCATGACAGCTCAGAAGGTCGCATTG
GTCACCGCCGGCGGCAGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MEMHQVRYFLTVAKLLNFSRAADECNVTQPSLSRAIKQLEAEIGGELFVRGPPSARLTALGHAVHPALKQCYDAALGARA
VAISMRQRPQTSLRLALTESIDLAPVIPHIQRIRNRFEHLDLTVQRGDGAKAAAMLQDGDVELVIAADLHKAGSHLHQLP
LFTEGFDLVVHTQHRLAGRPLVPAMELCREPLLVGAYCEHTPGILEGLRRHGVGAIRAGEVHAERDLLGLVAAGLGVALL
PRSAQLPAGLRRIAIAGLAIERTVSLYSLAGRPWSRIGAAVINQIRRSRTDRPQRRAPLDAAGENP

Sequences:

>Translated_306_residues
MEMHQVRYFLTVAKLLNFSRAADECNVTQPSLSRAIKQLEAEIGGELFVRGPPSARLTALGHAVHPALKQCYDAALGARA
VAISMRQRPQTSLRLALTESIDLAPVIPHIQRIRNRFEHLDLTVQRGDGAKAAAMLQDGDVELVIAADLHKAGSHLHQLP
LFTEGFDLVVHTQHRLAGRPLVPAMELCREPLLVGAYCEHTPGILEGLRRHGVGAIRAGEVHAERDLLGLVAAGLGVALL
PRSAQLPAGLRRIAIAGLAIERTVSLYSLAGRPWSRIGAAVINQIRRSRTDRPQRRAPLDAAGENP
>Mature_306_residues
MEMHQVRYFLTVAKLLNFSRAADECNVTQPSLSRAIKQLEAEIGGELFVRGPPSARLTALGHAVHPALKQCYDAALGARA
VAISMRQRPQTSLRLALTESIDLAPVIPHIQRIRNRFEHLDLTVQRGDGAKAAAMLQDGDVELVIAADLHKAGSHLHQLP
LFTEGFDLVVHTQHRLAGRPLVPAMELCREPLLVGAYCEHTPGILEGLRRHGVGAIRAGEVHAERDLLGLVAAGLGVALL
PRSAQLPAGLRRIAIAGLAIERTVSLYSLAGRPWSRIGAAVINQIRRSRTDRPQRRAPLDAAGENP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=29.9212598425197, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=273, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790399, Length=291, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=291, Percent_Identity=26.8041237113402, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=259, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=284, Percent_Identity=25.7042253521127, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33192; Mature: 33192

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMHQVRYFLTVAKLLNFSRAADECNVTQPSLSRAIKQLEAEIGGELFVRGPPSARLTAL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
GHAVHPALKQCYDAALGARAVAISMRQRPQTSLRLALTESIDLAPVIPHIQRIRNRFEHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHC
DLTVQRGDGAKAAAMLQDGDVELVIAADLHKAGSHLHQLPLFTEGFDLVVHTQHRLAGRP
EEEEECCCCCCEEHEEECCCEEEEEEECHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCC
LVPAMELCREPLLVGAYCEHTPGILEGLRRHGVGAIRAGEVHAERDLLGLVAAGLGVALL
CCHHHHHHHCCCEECHHHHCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHCC
PRSAQLPAGLRRIAIAGLAIERTVSLYSLAGRPWSRIGAAVINQIRRSRTDRPQRRAPLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
AAGENP
CCCCCC
>Mature Secondary Structure
MEMHQVRYFLTVAKLLNFSRAADECNVTQPSLSRAIKQLEAEIGGELFVRGPPSARLTAL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
GHAVHPALKQCYDAALGARAVAISMRQRPQTSLRLALTESIDLAPVIPHIQRIRNRFEHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHC
DLTVQRGDGAKAAAMLQDGDVELVIAADLHKAGSHLHQLPLFTEGFDLVVHTQHRLAGRP
EEEEECCCCCCEEHEEECCCEEEEEEECHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCC
LVPAMELCREPLLVGAYCEHTPGILEGLRRHGVGAIRAGEVHAERDLLGLVAAGLGVALL
CCHHHHHHHCCCEECHHHHCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHCC
PRSAQLPAGLRRIAIAGLAIERTVSLYSLAGRPWSRIGAAVINQIRRSRTDRPQRRAPLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCC
AAGENP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]