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The map label for this gene is pifA [H]

Identifier: 146343415

GI number: 146343415

Start: 6897716

End: 6899827

Strand: Direct

Name: pifA [H]

Synonym: BRADO6644

Alternate gene names: 146343415

Gene position: 6897716-6899827 (Clockwise)

Preceding gene: 146343414

Following gene: 146343416

Centisome position: 92.51

GC content: 63.16

Gene sequence:

>2112_bases
ATGACCAACCGACAGCTTGGCGATCCCGTGGTTCACAAGGATGACGACGCGTTCAACCGCTGGCCATTCAGCCAGCGGCT
GGCGCGCATGATCGCGACGTTCGACGCGCGCGAAGGCGCGCCGGTGATTGGAATTTACGGAAAATGGGGGTCGGGCAAGA
CCTCTGTGCTCAATTTCCTGAAAAAAGTGCTGGAGACCGAGCATGCCGATCAGGTCGTGCTGCTGTCCTTCAATCCCTGG
CTGTTCAAGGACAATGAGACGCTGCTGAAGCGGTTCTTCGAAGAGCTTTTCTTTGTAATGGGACGAAGCAGCCAGAGCAC
GCGCGAACAGTTTGGCCACATGCTGGCTGAGTTCGGCGGCGTGCTCGGTTTCCTGGCGAGCGGTGCAGGGACCGCGACAA
AGGAGCTGGGAAAGGCTTTCCTCAAAACGATTGAAGAGGTTCGCGATGACGTTCGCGAGGCGATGCAGACCGCCGACAAG
AAGGTCGTCGTGCTGATCGATGATCTCGACCGGCTCGACCGCGACGAAATCCTGCAGCTGCTCAAGATTGTCAGGCTCAC
GGCCAACTTCCCCAATGTGATCTATCTGCTCGCCTTCGACGACGAGATGGTGGGTCGCGCCGTTGCGCAGAAATTCGGCA
GCGATGGCGATGCCGGCCGCCAGTTCCTGGAGAAGATCGTCCAGTATCCGTATACGCTCCCGGCGGTCCCTCGCGAGCGC
CTGATGACCTTCGTCGAGCAGCAGGCGATCGCAGCCCTTGACCATGCCGGAGTGACGCTGCCCGACGACGACTGGCTGCG
GTTCAAGACGCTGGCTCGCGACGGCCTTGCCCGTCGTCTGACCACCCCGCGCCAGGCGATCCGCTACGGCAACGCGTTGA
GGTTCGCGCTGCCGATGCTGAAGGGTGAGGTGGATGTCGCCGATCAGATGAGCATCGAGGGCCTGCGGGTGCTGTACCCG
GAGCTCTACGCCGTCGTCCGCGACAACAAGCGCGTGTTCGCGGTGGAGGACGATCCCGAGGGCTATCCGAGCCTCGACGC
GGGGCCCAAGCGCCGGACGCTCTACGACAAGGACGGCATCGACGCCCGGATCGAGGCGGCCTTGCATAGCGTCGCTCCCG
ACGAAGCCGACTCCGCCCGAACACTCATCGCGGCCCTGTTCAAAACAATGCCGTTGCGGCCGCAAGGAATAGCGAGACCG
CGCTACTTCGACCGCTACTTCTCTTATGGGATCGCTGCTGATGAGATCGGTGATCTCGAGCTGGAGGAATTACTGCATGT
TTCGACCGGCGAGGCGGTGCAGCGGATCAAGACACTCTACGCCCGCAATCCGCGGGAGCTGCTGCAGCTTCTGCTGAAAC
GCGCCGACAACGCGAGCATGCAGACGCTGCTGACCCTCGCCCAGGCGCTGATCGGCGCAAGCCCGCATCTGGACGAAACG
TCGCAGATCCGGCCGCGGGACCTCGCTTATGTCATCGCTCAGCTGGTGTTGAGACCGAACGCCTTGGCCCAGTCCTGGGC
GGAAGATCACCCGGCGCGCCCCGTCGAGATCTATGAGATCTGGAAAGTAGGGCAGGATCTGCCGCTCTCGCTGGCCTTGT
ATTTCCGCGAAGCGATCCACCGTGCCGCCGCCGAGCACGAACGCGACCGAAACCGCCGCCAGCGCTCCGACACGTTGATC
AGGGAGGAGAGTTGGCGCGCTTTGGACAAGGCGCTAGCCAGCCGTCTCGCGGACGAGGCCAAGTTGGATTGGCGGGTGAT
GCTGCGCGAGCGGGCCGATGCGCCACCGCTGATGTTCGAATGGGGTCGCCTGGATCGGCCAGCCCAACGCGCTTGGCTGG
CCGCGCTGTTGACGGAGCCGGATGCCGTCGCGCCGTTCCTGCGCGCCAGCGTCGCCGATGTCAGCGCGCTGAGCCTCTCT
TTCGTATGGGAGGAACTGGGGTCGCTGATCGACATCGACACGCTCAAGAAAGCCGCACGCCATGCTGGCGAGTCGGTGAC
GGATCCGGAGACCGGCAACATCATCCGGACATTTCTGATGGGATGTGACGAGCGCAACGCCCGCGCCGCGCGGGCCTCAG
CAAGGGACACAAACGAGGCCGAATCGTCGTGA

Upstream 100 bases:

>100_bases
GCCTCACGGAGCGTCATTCACGTGACGCGAATTCCGGCGTAGGGTGCTGATCGCTGATCTCCGATGCCTGCGATCTTCCT
TTCTTGCCGGGGTGGATGTG

Downstream 100 bases:

>100_bases
CATGTGACATCTCGATGTCTGCTTACGCCGAGCCGCCCGGCACGGGTCTGTCGACCCGTTCAGATCGAACCTGATTGTGA
CCGGTCCGGTCCTTGCCCCG

Product: P-loop ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MTNRQLGDPVVHKDDDAFNRWPFSQRLARMIATFDAREGAPVIGIYGKWGSGKTSVLNFLKKVLETEHADQVVLLSFNPW
LFKDNETLLKRFFEELFFVMGRSSQSTREQFGHMLAEFGGVLGFLASGAGTATKELGKAFLKTIEEVRDDVREAMQTADK
KVVVLIDDLDRLDRDEILQLLKIVRLTANFPNVIYLLAFDDEMVGRAVAQKFGSDGDAGRQFLEKIVQYPYTLPAVPRER
LMTFVEQQAIAALDHAGVTLPDDDWLRFKTLARDGLARRLTTPRQAIRYGNALRFALPMLKGEVDVADQMSIEGLRVLYP
ELYAVVRDNKRVFAVEDDPEGYPSLDAGPKRRTLYDKDGIDARIEAALHSVAPDEADSARTLIAALFKTMPLRPQGIARP
RYFDRYFSYGIAADEIGDLELEELLHVSTGEAVQRIKTLYARNPRELLQLLLKRADNASMQTLLTLAQALIGASPHLDET
SQIRPRDLAYVIAQLVLRPNALAQSWAEDHPARPVEIYEIWKVGQDLPLSLALYFREAIHRAAAEHERDRNRRQRSDTLI
REESWRALDKALASRLADEAKLDWRVMLRERADAPPLMFEWGRLDRPAQRAWLAALLTEPDAVAPFLRASVADVSALSLS
FVWEELGSLIDIDTLKKAARHAGESVTDPETGNIIRTFLMGCDERNARAARASARDTNEAESS

Sequences:

>Translated_703_residues
MTNRQLGDPVVHKDDDAFNRWPFSQRLARMIATFDAREGAPVIGIYGKWGSGKTSVLNFLKKVLETEHADQVVLLSFNPW
LFKDNETLLKRFFEELFFVMGRSSQSTREQFGHMLAEFGGVLGFLASGAGTATKELGKAFLKTIEEVRDDVREAMQTADK
KVVVLIDDLDRLDRDEILQLLKIVRLTANFPNVIYLLAFDDEMVGRAVAQKFGSDGDAGRQFLEKIVQYPYTLPAVPRER
LMTFVEQQAIAALDHAGVTLPDDDWLRFKTLARDGLARRLTTPRQAIRYGNALRFALPMLKGEVDVADQMSIEGLRVLYP
ELYAVVRDNKRVFAVEDDPEGYPSLDAGPKRRTLYDKDGIDARIEAALHSVAPDEADSARTLIAALFKTMPLRPQGIARP
RYFDRYFSYGIAADEIGDLELEELLHVSTGEAVQRIKTLYARNPRELLQLLLKRADNASMQTLLTLAQALIGASPHLDET
SQIRPRDLAYVIAQLVLRPNALAQSWAEDHPARPVEIYEIWKVGQDLPLSLALYFREAIHRAAAEHERDRNRRQRSDTLI
REESWRALDKALASRLADEAKLDWRVMLRERADAPPLMFEWGRLDRPAQRAWLAALLTEPDAVAPFLRASVADVSALSLS
FVWEELGSLIDIDTLKKAARHAGESVTDPETGNIIRTFLMGCDERNARAARASARDTNEAESS
>Mature_702_residues
TNRQLGDPVVHKDDDAFNRWPFSQRLARMIATFDAREGAPVIGIYGKWGSGKTSVLNFLKKVLETEHADQVVLLSFNPWL
FKDNETLLKRFFEELFFVMGRSSQSTREQFGHMLAEFGGVLGFLASGAGTATKELGKAFLKTIEEVRDDVREAMQTADKK
VVVLIDDLDRLDRDEILQLLKIVRLTANFPNVIYLLAFDDEMVGRAVAQKFGSDGDAGRQFLEKIVQYPYTLPAVPRERL
MTFVEQQAIAALDHAGVTLPDDDWLRFKTLARDGLARRLTTPRQAIRYGNALRFALPMLKGEVDVADQMSIEGLRVLYPE
LYAVVRDNKRVFAVEDDPEGYPSLDAGPKRRTLYDKDGIDARIEAALHSVAPDEADSARTLIAALFKTMPLRPQGIARPR
YFDRYFSYGIAADEIGDLELEELLHVSTGEAVQRIKTLYARNPRELLQLLLKRADNASMQTLLTLAQALIGASPHLDETS
QIRPRDLAYVIAQLVLRPNALAQSWAEDHPARPVEIYEIWKVGQDLPLSLALYFREAIHRAAAEHERDRNRRQRSDTLIR
EESWRALDKALASRLADEAKLDWRVMLRERADAPPLMFEWGRLDRPAQRAWLAALLTEPDAVAPFLRASVADVSALSLSF
VWEELGSLIDIDTLKKAARHAGESVTDPETGNIIRTFLMGCDERNARAARASARDTNEAESS

Specific function: Responsible for the exclusion of phage T7 by plasmid F. Growth of bacteriophage T7 is inhibited in cells of E.coli that carries the plasmid F [H]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KAP NTPase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011646 [H]

Pfam domain/function: PF07693 KAP_NTPase [H]

EC number: NA

Molecular weight: Translated: 79240; Mature: 79109

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRQLGDPVVHKDDDAFNRWPFSQRLARMIATFDAREGAPVIGIYGKWGSGKTSVLNFL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHH
KKVLETEHADQVVLLSFNPWLFKDNETLLKRFFEELFFVMGRSSQSTREQFGHMLAEFGG
HHHHCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VLGFLASGAGTATKELGKAFLKTIEEVRDDVREAMQTADKKVVVLIDDLDRLDRDEILQL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
LKIVRLTANFPNVIYLLAFDDEMVGRAVAQKFGSDGDAGRQFLEKIVQYPYTLPAVPRER
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LMTFVEQQAIAALDHAGVTLPDDDWLRFKTLARDGLARRLTTPRQAIRYGNALRFALPML
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
KGEVDVADQMSIEGLRVLYPELYAVVRDNKRVFAVEDDPEGYPSLDAGPKRRTLYDKDGI
CCCCCHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DARIEAALHSVAPDEADSARTLIAALFKTMPLRPQGIARPRYFDRYFSYGIAADEIGDLE
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCC
LEELLHVSTGEAVQRIKTLYARNPRELLQLLLKRADNASMQTLLTLAQALIGASPHLDET
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
SQIRPRDLAYVIAQLVLRPNALAQSWAEDHPARPVEIYEIWKVGQDLPLSLALYFREAIH
CCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
RAAAEHERDRNRRQRSDTLIREESWRALDKALASRLADEAKLDWRVMLRERADAPPLMFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEE
WGRLDRPAQRAWLAALLTEPDAVAPFLRASVADVSALSLSFVWEELGSLIDIDTLKKAAR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HAGESVTDPETGNIIRTFLMGCDERNARAARASARDTNEAESS
HCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNRQLGDPVVHKDDDAFNRWPFSQRLARMIATFDAREGAPVIGIYGKWGSGKTSVLNFL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHH
KKVLETEHADQVVLLSFNPWLFKDNETLLKRFFEELFFVMGRSSQSTREQFGHMLAEFGG
HHHHCCCCCCCEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VLGFLASGAGTATKELGKAFLKTIEEVRDDVREAMQTADKKVVVLIDDLDRLDRDEILQL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
LKIVRLTANFPNVIYLLAFDDEMVGRAVAQKFGSDGDAGRQFLEKIVQYPYTLPAVPRER
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LMTFVEQQAIAALDHAGVTLPDDDWLRFKTLARDGLARRLTTPRQAIRYGNALRFALPML
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHH
KGEVDVADQMSIEGLRVLYPELYAVVRDNKRVFAVEDDPEGYPSLDAGPKRRTLYDKDGI
CCCCCHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
DARIEAALHSVAPDEADSARTLIAALFKTMPLRPQGIARPRYFDRYFSYGIAADEIGDLE
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHCCCCCC
LEELLHVSTGEAVQRIKTLYARNPRELLQLLLKRADNASMQTLLTLAQALIGASPHLDET
HHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
SQIRPRDLAYVIAQLVLRPNALAQSWAEDHPARPVEIYEIWKVGQDLPLSLALYFREAIH
CCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
RAAAEHERDRNRRQRSDTLIREESWRALDKALASRLADEAKLDWRVMLRERADAPPLMFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEE
WGRLDRPAQRAWLAALLTEPDAVAPFLRASVADVSALSLSFVWEELGSLIDIDTLKKAAR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
HAGESVTDPETGNIIRTFLMGCDERNARAARASARDTNEAESS
HCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 5283385 [H]