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The map label for this gene is gltD [H]

Identifier: 146343049

GI number: 146343049

Start: 6485423

End: 6486871

Strand: Direct

Name: gltD [H]

Synonym: BRADO6240

Alternate gene names: 146343049

Gene position: 6485423-6486871 (Clockwise)

Preceding gene: 146343048

Following gene: 146343053

Centisome position: 86.98

GC content: 66.53

Gene sequence:

>1449_bases
ATGGGCAAGGTCACAGGTTTTCTCGAAATCGAGCGGCACGATCGCAAATACGCGCCGGTTGCCGAGCGGCTGAAGCATTT
CCAGGAATTCGTGGTGCCGCTCTCCGAGAAGGAGCTGCGCGACCAGGCGGCGCGCTGCATGAATTGCGGCATCCCCTTTT
GCCACGGCACCGGCTCGGCCGCGCCGGGCACCACCGGCTGCCCGGTCAACAACCAGATCCCGGACTGGAACGACCTGATC
TACAACGGCAACTGGGAAGAGGCGTCGCGCAACCTGCACTCGACCAACAACTTCCCGGAGTTCACCGGCCGCATCTGCCC
CGCTCCGTGCGAGGCGTCGTGCACCTTGAACATCGACGACAACCCGGTGACCATCAAGACGATCGAATGCGCCATCGTCG
ACAAGGCCTGGGAGAACGGCTGGCTCAAGCCGGAGCCGGCCGCGGTCAAGACCGGCAAGAAGGTCGCGATCGTCGGCGCC
GGCCCTGCCGGCCTCGCCGCTGCGCAGCAGCTCGCCCGCGCCGGTCACGAGGTGCATGTCTATGAGAAGCACGCCAAGGC
CGGCGGCCTTCTTCGTTACGGCATTCCCGACTTTAAGATGGAGAAGGGCATCATCGACCGCCGCATCGCTCAGATGGAAG
GCGAAGGCGTCGTCTTCCACTACGGCGCCCATGTCGGCGGCAAGGACGGCATCGATCCGAAGTCGCTGCTGAAGGATTAT
GACGCCGTGGCGCTGACCGGCGGCGCCGAGGCGCCACGCGACCTGCCGATCCCCGGCCGCGACCTTTCCGGCATCCACTA
CGCGATGGATTTCCTGCCGCAGCAGAACCGCCGCGTCGGCAACGAGCCGGTCAACGCCACCGAGATCCTCGCGGGCGGAA
AGCATGTCGTCGTGATCGGCGGCGGCGACACGGGCTCTGACTGCATCGGCACCTCGCTGCGCCAGGGCGCCCTCTCCGTC
ACCCAGCTCGAGATCATGCCGGCGCCGCCGGAGCGTGAGAACAAGGCGCTCACTTGGCCGAACTGGCCCTTGAAGATGCG
GACCTCGTCGAGCCAGGCCGAAGGCGCGGCGCGCGAATTCGCAGTGCTGACGCAGACCTTCGAAGGCGAGAACGGCGCGG
TCAAGAAGATGCACTGCGTCCGGGTCGATGCGCAGTTCAAGCCGATCCCCGGCACCGAGTTCGAGCTCGCTGCCGACCTC
GTGCTGCTGGCGATGGGCTTCGTCCATCCGGTGCACGAGGGCCTGCTCAAGAGCCTCGGCGTCGACCTCGACCCGCGCGG
CAACGTCAAGGCGAACCTGTCGGACTACCAGACCTCGCTGCCGAAGGTGTTCTCCGCCGGCGACATGCGCCGCGGCCAGT
CCCTGGTCGTCTGGGCGATCCGCGAAGGCCGCCTTTGCGCCCAGGCCGTCGACAAGTTCTTGATGGGCAAGACCGAGCTG
CCGCGCTGA

Upstream 100 bases:

>100_bases
CGTACCGCGCAATTTTTCGTACGATACAGATCGGACGCTGGTCATCAGTCAAAGGCCGGACGGTATTTTCGATCTCGTGG
GTGGTGAGGATTAGACGCAG

Downstream 100 bases:

>100_bases
GCTGCGTGTCAGCGCGTAGATAATTGCCTTTAATAGATTTTCCGGACTGCCTACCCCGAAGCCGGTGCGCTCCCCTCCCC
CTTGCGGGGAGGGGTCGGGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MGKVTGFLEIERHDRKYAPVAERLKHFQEFVVPLSEKELRDQAARCMNCGIPFCHGTGSAAPGTTGCPVNNQIPDWNDLI
YNGNWEEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDKAWENGWLKPEPAAVKTGKKVAIVGA
GPAGLAAAQQLARAGHEVHVYEKHAKAGGLLRYGIPDFKMEKGIIDRRIAQMEGEGVVFHYGAHVGGKDGIDPKSLLKDY
DAVALTGGAEAPRDLPIPGRDLSGIHYAMDFLPQQNRRVGNEPVNATEILAGGKHVVVIGGGDTGSDCIGTSLRQGALSV
TQLEIMPAPPERENKALTWPNWPLKMRTSSSQAEGAAREFAVLTQTFEGENGAVKKMHCVRVDAQFKPIPGTEFELAADL
VLLAMGFVHPVHEGLLKSLGVDLDPRGNVKANLSDYQTSLPKVFSAGDMRRGQSLVVWAIREGRLCAQAVDKFLMGKTEL
PR

Sequences:

>Translated_482_residues
MGKVTGFLEIERHDRKYAPVAERLKHFQEFVVPLSEKELRDQAARCMNCGIPFCHGTGSAAPGTTGCPVNNQIPDWNDLI
YNGNWEEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDKAWENGWLKPEPAAVKTGKKVAIVGA
GPAGLAAAQQLARAGHEVHVYEKHAKAGGLLRYGIPDFKMEKGIIDRRIAQMEGEGVVFHYGAHVGGKDGIDPKSLLKDY
DAVALTGGAEAPRDLPIPGRDLSGIHYAMDFLPQQNRRVGNEPVNATEILAGGKHVVVIGGGDTGSDCIGTSLRQGALSV
TQLEIMPAPPERENKALTWPNWPLKMRTSSSQAEGAAREFAVLTQTFEGENGAVKKMHCVRVDAQFKPIPGTEFELAADL
VLLAMGFVHPVHEGLLKSLGVDLDPRGNVKANLSDYQTSLPKVFSAGDMRRGQSLVVWAIREGRLCAQAVDKFLMGKTEL
PR
>Mature_481_residues
GKVTGFLEIERHDRKYAPVAERLKHFQEFVVPLSEKELRDQAARCMNCGIPFCHGTGSAAPGTTGCPVNNQIPDWNDLIY
NGNWEEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDDNPVTIKTIECAIVDKAWENGWLKPEPAAVKTGKKVAIVGAG
PAGLAAAQQLARAGHEVHVYEKHAKAGGLLRYGIPDFKMEKGIIDRRIAQMEGEGVVFHYGAHVGGKDGIDPKSLLKDYD
AVALTGGAEAPRDLPIPGRDLSGIHYAMDFLPQQNRRVGNEPVNATEILAGGKHVVVIGGGDTGSDCIGTSLRQGALSVT
QLEIMPAPPERENKALTWPNWPLKMRTSSSQAEGAAREFAVLTQTFEGENGAVKKMHCVRVDAQFKPIPGTEFELAADLV
LLAMGFVHPVHEGLLKSLGVDLDPRGNVKANLSDYQTSLPKVFSAGDMRRGQSLVVWAIREGRLCAQAVDKFLMGKTELP
R

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=309, Percent_Identity=27.1844660194175, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1789606, Length=494, Percent_Identity=37.8542510121458, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1788811, Length=478, Percent_Identity=36.8200836820084, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87082180, Length=466, Percent_Identity=33.4763948497854, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1788468, Length=443, Percent_Identity=29.1196388261851, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1789243, Length=424, Percent_Identity=27.5943396226415, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=500, Percent_Identity=42, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984108, Length=303, Percent_Identity=28.0528052805281, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=498, Percent_Identity=42.9718875502008, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574881, Length=493, Percent_Identity=49.8985801217039, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665539, Length=493, Percent_Identity=49.8985801217039, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665547, Length=493, Percent_Identity=49.8985801217039, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=493, Percent_Identity=49.8985801217039, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=301, Percent_Identity=29.2358803986711, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18858217, Length=301, Percent_Identity=29.2358803986711, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52223; Mature: 52092

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEIERHDRKYAPVAERLKHFQEFVVPLSEKELRDQAARCMNCGIPFCHGTGSA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCC
APGTTGCPVNNQIPDWNDLIYNGNWEEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDD
CCCCCCCCCCCCCCCHHCEEECCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEECC
NPVTIKTIECAIVDKAWENGWLKPEPAAVKTGKKVAIVGAGPAGLAAAQQLARAGHEVHV
CCEEEEEEEEEEEEHHHHCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
YEKHAKAGGLLRYGIPDFKMEKGIIDRRIAQMEGEGVVFHYGAHVGGKDGIDPKSLLKDY
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCC
DAVALTGGAEAPRDLPIPGRDLSGIHYAMDFLPQQNRRVGNEPVNATEILAGGKHVVVIG
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEEE
GGDTGSDCIGTSLRQGALSVTQLEIMPAPPERENKALTWPNWPLKMRTSSSQAEGAAREF
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHH
AVLTQTFEGENGAVKKMHCVRVDAQFKPIPGTEFELAADLVLLAMGFVHPVHEGLLKSLG
HHHHHEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLDPRGNVKANLSDYQTSLPKVFSAGDMRRGQSLVVWAIREGRLCAQAVDKFLMGKTEL
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
PR
CC
>Mature Secondary Structure 
GKVTGFLEIERHDRKYAPVAERLKHFQEFVVPLSEKELRDQAARCMNCGIPFCHGTGSA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCC
APGTTGCPVNNQIPDWNDLIYNGNWEEASRNLHSTNNFPEFTGRICPAPCEASCTLNIDD
CCCCCCCCCCCCCCCHHCEEECCCHHHHHCCCCCCCCCCHHCCCCCCCCCCCEEEEEECC
NPVTIKTIECAIVDKAWENGWLKPEPAAVKTGKKVAIVGAGPAGLAAAQQLARAGHEVHV
CCEEEEEEEEEEEEHHHHCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEE
YEKHAKAGGLLRYGIPDFKMEKGIIDRRIAQMEGEGVVFHYGAHVGGKDGIDPKSLLKDY
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCC
DAVALTGGAEAPRDLPIPGRDLSGIHYAMDFLPQQNRRVGNEPVNATEILAGGKHVVVIG
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEEEEE
GGDTGSDCIGTSLRQGALSVTQLEIMPAPPERENKALTWPNWPLKMRTSSSQAEGAAREF
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHH
AVLTQTFEGENGAVKKMHCVRVDAQFKPIPGTEFELAADLVLLAMGFVHPVHEGLLKSLG
HHHHHEECCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDLDPRGNVKANLSDYQTSLPKVFSAGDMRRGQSLVVWAIREGRLCAQAVDKFLMGKTEL
CCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCC
PR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]