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The map label for this gene is 146342605

Identifier: 146342605

GI number: 146342605

Start: 5970906

End: 5972126

Strand: Direct

Name: 146342605

Synonym: BRADO5769

Alternate gene names: NA

Gene position: 5970906-5972126 (Clockwise)

Preceding gene: 146342602

Following gene: 146342607

Centisome position: 80.08

GC content: 63.23

Gene sequence:

>1221_bases
ATGGCGCTGCTGTACCAAGCCTATCAGAACCACATGGACCTGACCGAGCCATGGCGCCAGGGCGCCGCGGCCGCCATGCG
CTATCTCAATCTGATCCCGTCAGGCCTCTCCAGCCGCGCGATGCGGCGGCTGTCGGCAGCACTGGAATTGATCTCGCGCT
CGGCGCTCACCTATGCGCGTCCGGCCTATGGCATCGACAGCGTCATGGTCGGCAATCGCGAGCTCGGCGTCACCGAGGAG
GTGACGTACAAGACCCCGTTCGGCTCGCTGCTCCATTTCAAAAAGGACGGCGCGGCGGATCAGCCCAAGGTGCTGCTGGT
CGCGCCGATGTCGGGTCACTTCGCGACGCTGTTGCGCGGCACGGTGAAGACACTGCTGCAGGATCACGACGTGTACATCA
CCGACTGGCACAATCCGCGCGATATTCCGCCGGGCCATGGCCGCTTCGGGCTGGAAGATTACACTGATCATCTCATCAGC
TTCCTGGGCCAACTCGGGCCACGGCCGCACATGGTGGCGATCTGCCAGCCCTCGGTGTCGGCGCTGGCGGCGACCGCCGT
GATGTGCGAGGGCAACCATCCGTCGCGGCCGGCGACGCTCACCCTGATGGCGGGGCCGATCGACACGCGCATCCAGCCGA
CCAAGGTCAACGAGTTCGCCAAGAGCAAGCCGATCGAGTGGTTCGAGGACAATCTGATCCACTACGTGCCCTTTCAGTGC
CGTGGCGCGTTCCGGAAAGTCTATCCGGGCTTCGTGCAGCTCACGGCCTTCGTGTCGATGAATCTCGAGCGCCACGTCAA
GCAGCACATGGATCTCGCCAATCATCTCGCCAAGGGCGAGGTCGAGAAGGCCGAGTCGATCAAGACATTCTATGACGAGT
ATTTCGCCGTGATGGACCTGCCGGCGGAGTTCTACATCGAGACCGTGCGCGACGTCTTCCAGGAGCATCTGCTGCCCCAG
GGCAAGCTCATGCATCGCGGCAACCCTGTCGATCCGAAGGCGGTGCGCCGCATCGGACTGATGACGGTCGAGGGCGAGCG
CGACGACATCTGCTCGATCGGCCAGACGCTGTCGGCCCAGGACATCTGCACCAATGTGCGCGCGTATCGCCGCGTGCATC
ACATGCAGGCCGGCGTCGGCCATTACGGCGTGTTCAGCGGCAAGAAGTGGAACAACGAGATCTATCCGCTGCTGCGCGAT
TTCGTCCACGTCAATTCGTGA

Upstream 100 bases:

>100_bases
GCAGATTCATTGAGCAAGCGACATGCCAGTTTTTCAGGCAAGACTCCGCCGCGCTGGCTCCGATATTGCATATGCACAAG
CATGTAATGGATCGGACCCG

Downstream 100 bases:

>100_bases
AGTCAGTTCGTCAGGTCAGTTCGTCAGGTCAGTTCGTGAGCGGACCTCGGCCACGCTGCACGCGCTGCAGCGTGGCTGAC
GGCGTCTCTCCGAACTTGTC

Product: putative Poly-beta-hydroxyalkanoate/butyrate (PHA/PHB) depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MALLYQAYQNHMDLTEPWRQGAAAAMRYLNLIPSGLSSRAMRRLSAALELISRSALTYARPAYGIDSVMVGNRELGVTEE
VTYKTPFGSLLHFKKDGAADQPKVLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPPGHGRFGLEDYTDHLIS
FLGQLGPRPHMVAICQPSVSALAATAVMCEGNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIEWFEDNLIHYVPFQC
RGAFRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAESIKTFYDEYFAVMDLPAEFYIETVRDVFQEHLLPQ
GKLMHRGNPVDPKAVRRIGLMTVEGERDDICSIGQTLSAQDICTNVRAYRRVHHMQAGVGHYGVFSGKKWNNEIYPLLRD
FVHVNS

Sequences:

>Translated_406_residues
MALLYQAYQNHMDLTEPWRQGAAAAMRYLNLIPSGLSSRAMRRLSAALELISRSALTYARPAYGIDSVMVGNRELGVTEE
VTYKTPFGSLLHFKKDGAADQPKVLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPPGHGRFGLEDYTDHLIS
FLGQLGPRPHMVAICQPSVSALAATAVMCEGNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIEWFEDNLIHYVPFQC
RGAFRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAESIKTFYDEYFAVMDLPAEFYIETVRDVFQEHLLPQ
GKLMHRGNPVDPKAVRRIGLMTVEGERDDICSIGQTLSAQDICTNVRAYRRVHHMQAGVGHYGVFSGKKWNNEIYPLLRD
FVHVNS
>Mature_405_residues
ALLYQAYQNHMDLTEPWRQGAAAAMRYLNLIPSGLSSRAMRRLSAALELISRSALTYARPAYGIDSVMVGNRELGVTEEV
TYKTPFGSLLHFKKDGAADQPKVLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPPGHGRFGLEDYTDHLISF
LGQLGPRPHMVAICQPSVSALAATAVMCEGNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIEWFEDNLIHYVPFQCR
GAFRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAESIKTFYDEYFAVMDLPAEFYIETVRDVFQEHLLPQG
KLMHRGNPVDPKAVRRIGLMTVEGERDDICSIGQTLSAQDICTNVRAYRRVHHMQAGVGHYGVFSGKKWNNEIYPLLRDF
VHVNS

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45686; Mature: 45555

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLYQAYQNHMDLTEPWRQGAAAAMRYLNLIPSGLSSRAMRRLSAALELISRSALTYAR
CCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PAYGIDSVMVGNRELGVTEEVTYKTPFGSLLHFKKDGAADQPKVLLVAPMSGHFATLLRG
CCCCCCCEEECCCCCCCCCCEEECCCCHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHH
TVKTLLQDHDVYITDWHNPRDIPPGHGRFGLEDYTDHLISFLGQLGPRPHMVAICQPSVS
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
ALAATAVMCEGNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIEWFEDNLIHYVPFQC
HHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHCCCEEECCHHH
RGAFRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAESIKTFYDEYFAVMDL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PAEFYIETVRDVFQEHLLPQGKLMHRGNPVDPKAVRRIGLMTVEGERDDICSIGQTLSAQ
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCEEECCCCCHHHHHHCCCCHH
DICTNVRAYRRVHHMQAGVGHYGVFSGKKWNNEIYPLLRDFVHVNS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLYQAYQNHMDLTEPWRQGAAAAMRYLNLIPSGLSSRAMRRLSAALELISRSALTYAR
CEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PAYGIDSVMVGNRELGVTEEVTYKTPFGSLLHFKKDGAADQPKVLLVAPMSGHFATLLRG
CCCCCCCEEECCCCCCCCCCEEECCCCHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHH
TVKTLLQDHDVYITDWHNPRDIPPGHGRFGLEDYTDHLISFLGQLGPRPHMVAICQPSVS
HHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
ALAATAVMCEGNHPSRPATLTLMAGPIDTRIQPTKVNEFAKSKPIEWFEDNLIHYVPFQC
HHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHCCCEEECCHHH
RGAFRKVYPGFVQLTAFVSMNLERHVKQHMDLANHLAKGEVEKAESIKTFYDEYFAVMDL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PAEFYIETVRDVFQEHLLPQGKLMHRGNPVDPKAVRRIGLMTVEGERDDICSIGQTLSAQ
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCEEECCCCCHHHHHHCCCCHH
DICTNVRAYRRVHHMQAGVGHYGVFSGKKWNNEIYPLLRDFVHVNS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA