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The map label for this gene is thiG

Identifier: 146342549

GI number: 146342549

Start: 5913697

End: 5914479

Strand: Direct

Name: thiG

Synonym: BRADO5711

Alternate gene names: 146342549

Gene position: 5913697-5914479 (Clockwise)

Preceding gene: 146342548

Following gene: 146342550

Centisome position: 79.31

GC content: 67.82

Gene sequence:

>783_bases
ATGCTGAAATTTTACGACAAGACCGTCACCTCCCGCCTCCTCATCGGCACCGCGCTGTATCCCTCGCCTGCCATCATGCA
GGACGCGATCCGTGCCTCCGGCGCCGAGATCGTCACGGTGTCGTTGCGGCGGGAGGCCGCGGGCGGCAAGACGGGCGATG
CGTTCTGGAAGCTGATCAACGAGCTGAACGTCACGGTGCTGCCGAACACGGCCGGCTGCCGCAGCGTGCGCGAGGCCGTC
ACTACGGCCAAGCTCGCGCGCGAATTGTTCGGCACGTCCTGGATCAAGCTCGAGGTCATCGGCGACAACGACACCCTGCA
ACCCGACGTCATAGGCCTGGTCGAGGCCGCCACCATCCTGATTAAGGACGGCTTCGAGGTCTTCCCCTACTGCACCGAGG
ACCTCACCGTCGCCACGCGCCTGGTCGATGCCGGCTGCAGGGTCGTGATGCCCTGGGCGGCGCCGATCGGCTCGGCCAAG
GGCATCATCAACCGCGACGCGCTGAAGCTGCTGCGCGACCGCCTGCCCGATATCACGCTCGTCGTCGATGCCGGCCTCGG
CGCGCCCTCGCACGCCGCGGGGGCGCTGGAGCTCGGCTATGACGCCGTGCTGCTCAACACCGCGATCGCGAAAGCCGCCG
ATCCGGTCGCGATGGCGCGCGCCTTCAGGCTCGGAGTCGAAGCCGGCCGCACCGCCTACGAGGCCGGTCTGATGGACGCC
CGCGATTTCGCCTCGCCCTCCACCCCTGTCGTTGGGACTCCGTTCTGGCATGCCGTATCCTGA

Upstream 100 bases:

>100_bases
TCGGCTCAACACGACTGCCTCTGGAATACTGGATCCCCGCCTGCGCGGGGATGACGGCGTTCCTTGTGGCGACCACCGTC
CGACATGACGGAGACAATCG

Downstream 100 bases:

>100_bases
TCGCTTCTATCCCGTCGTCGACAGCCTCGCCTGGGTCGAGCGTCTCACCCGTCTCGGCGTCGGCACCGTGCAATTGCGCG
CGAAGAATCTCGACGACGCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLKFYDKTVTSRLLIGTALYPSPAIMQDAIRASGAEIVTVSLRREAAGGKTGDAFWKLINELNVTVLPNTAGCRSVREAV
TTAKLARELFGTSWIKLEVIGDNDTLQPDVIGLVEAATILIKDGFEVFPYCTEDLTVATRLVDAGCRVVMPWAAPIGSAK
GIINRDALKLLRDRLPDITLVVDAGLGAPSHAAGALELGYDAVLLNTAIAKAADPVAMARAFRLGVEAGRTAYEAGLMDA
RDFASPSTPVVGTPFWHAVS

Sequences:

>Translated_260_residues
MLKFYDKTVTSRLLIGTALYPSPAIMQDAIRASGAEIVTVSLRREAAGGKTGDAFWKLINELNVTVLPNTAGCRSVREAV
TTAKLARELFGTSWIKLEVIGDNDTLQPDVIGLVEAATILIKDGFEVFPYCTEDLTVATRLVDAGCRVVMPWAAPIGSAK
GIINRDALKLLRDRLPDITLVVDAGLGAPSHAAGALELGYDAVLLNTAIAKAADPVAMARAFRLGVEAGRTAYEAGLMDA
RDFASPSTPVVGTPFWHAVS
>Mature_260_residues
MLKFYDKTVTSRLLIGTALYPSPAIMQDAIRASGAEIVTVSLRREAAGGKTGDAFWKLINELNVTVLPNTAGCRSVREAV
TTAKLARELFGTSWIKLEVIGDNDTLQPDVIGLVEAATILIKDGFEVFPYCTEDLTVATRLVDAGCRVVMPWAAPIGSAK
GIINRDALKLLRDRLPDITLVVDAGLGAPSHAAGALELGYDAVLLNTAIAKAADPVAMARAFRLGVEAGRTAYEAGLMDA
RDFASPSTPVVGTPFWHAVS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=46.031746031746, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BRASO (A4YZQ4)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001207597.1
- ProteinModelPortal:   A4YZQ4
- SMR:   A4YZQ4
- STRING:   A4YZQ4
- GeneID:   5117851
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO5711
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   PIIIDAG
- ProtClustDB:   PRK00208
- BioCyc:   BSP376:BRADO5711-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27582; Mature: 27582

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFYDKTVTSRLLIGTALYPSPAIMQDAIRASGAEIVTVSLRREAAGGKTGDAFWKLIN
CCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEHHCCCCCCHHHHHHHHH
ELNVTVLPNTAGCRSVREAVTTAKLARELFGTSWIKLEVIGDNDTLQPDVIGLVEAATIL
HCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHE
IKDGFEVFPYCTEDLTVATRLVDAGCRVVMPWAAPIGSAKGIINRDALKLLRDRLPDITL
EECCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VVDAGLGAPSHAAGALELGYDAVLLNTAIAKAADPVAMARAFRLGVEAGRTAYEAGLMDA
EEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCH
RDFASPSTPVVGTPFWHAVS
HHCCCCCCCEECCCCHHCCC
>Mature Secondary Structure
MLKFYDKTVTSRLLIGTALYPSPAIMQDAIRASGAEIVTVSLRREAAGGKTGDAFWKLIN
CCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEHHCCCCCCHHHHHHHHH
ELNVTVLPNTAGCRSVREAVTTAKLARELFGTSWIKLEVIGDNDTLQPDVIGLVEAATIL
HCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHE
IKDGFEVFPYCTEDLTVATRLVDAGCRVVMPWAAPIGSAKGIINRDALKLLRDRLPDITL
EECCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEE
VVDAGLGAPSHAAGALELGYDAVLLNTAIAKAADPVAMARAFRLGVEAGRTAYEAGLMDA
EEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCH
RDFASPSTPVVGTPFWHAVS
HHCCCCCCCEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA