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The map label for this gene is thiO [H]

Identifier: 146342547

GI number: 146342547

Start: 5912167

End: 5913180

Strand: Direct

Name: thiO [H]

Synonym: BRADO5709

Alternate gene names: 146342547

Gene position: 5912167-5913180 (Clockwise)

Preceding gene: 146342546

Following gene: 146342548

Centisome position: 79.29

GC content: 68.15

Gene sequence:

>1014_bases
ATGCAGCAGACACCCACGCGCGGGGATCGCCCCGTATCCATCATTGGCGCCGGTATTGCGGGCGCCTGGCAGGCGCTGCT
GTTCGCGCGCGCCGGCCACGATGTCACCTTGCACGAGCGCAGCGACGCGGCGCTGATGCTCTCGACAAGCCATTGGGCCG
GCGGCATGCTGGCGCCCTATTGCGAGAGCGAAGTCTCCGAACCGATCATCAGCCGCCTCGGCCTCGCCTCGCTCGACATC
TGGCGGCGCGAGATGCCCGAGACGCCGTTCAATGGCTCGCTGGTCATCGCGCATGCCCGCGACCGCGCCGATTACGACCG
CTTCGCCCGCCGCACCTCCGGCTATCAGCGGCTCGATGCGGCGGCGCTCGCGGAGCTGGAGCCGTCGCTGGAGGGCCGTT
TCCGCGAAGGCCTGTTCTATCCAACCGAGGGCCATGTCGAGCCGCGCCGCGTGTTGCCGAAGCTGCACCAGCGCATCGTC
GAGGCCGGCGGCCGCGTGCTGTTCAACAGCAATGTCAGCACTGATGATCTCGACGGCCTCGTGATCGACTGTCGCGGTCT
CGCCGCGCGCCAGGCCGAGCCTGAGCTGCGCGGCGTCAAGGGCGAGATGATCCTGATCGAGACCTCCGAGGTGAGCCTCG
CGCGTCCGGTGCGGCTGATGCATCCGCGCTTTCCGCTCTATCTGATCCCGCGCGAGGACAATCTGTTCATGCTGGGTGCG
ACCTCGCTGGAAGCCGAGGACACCGGCGTCAGCCTGCGCTCGGCGCTGGAGCTGCTGGGCGCGGCCTATTCGGTGCATCC
GGCGTTCGGCGAGGCCCGCATCCTCGAATTCGGCTCGGGCCTGCGGCCGGCCTATCCCGACAATCTGCCGCGCATCACGG
TCAGAAGCGAGCGCGAGATCCGCGTCAACGGCCTCTATCGCCACGGCTTCCTGATCGCGCCCGCGCTGGCGCAGGCGACA
TTGAACTACGTGGCGCATGGCCAGATCGACAACGAGGTGATGCGATGCGCGTGA

Upstream 100 bases:

>100_bases
AAGCGCGTTCCGAGGGGAGCTCCGAGGGGGAGCTGAGATACCGCACGTGATGCGGTGACCCTTTGAACCTGATCCGGGTC
ATGCCGGCGAAGGGACAGGG

Downstream 100 bases:

>100_bases
CGGTCAATGGCGAGGCCCGCGACATCAGCGCGACCGACGTCGCCGCGCTGCTGTCGGAGCTCGACTACGAGGGCACGCAT
TTCGCGATCGCGCTGAACTA

Product: thiamine biosynthesis oxidoreductase thiO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQQTPTRGDRPVSIIGAGIAGAWQALLFARAGHDVTLHERSDAALMLSTSHWAGGMLAPYCESEVSEPIISRLGLASLDI
WRREMPETPFNGSLVIAHARDRADYDRFARRTSGYQRLDAAALAELEPSLEGRFREGLFYPTEGHVEPRRVLPKLHQRIV
EAGGRVLFNSNVSTDDLDGLVIDCRGLAARQAEPELRGVKGEMILIETSEVSLARPVRLMHPRFPLYLIPREDNLFMLGA
TSLEAEDTGVSLRSALELLGAAYSVHPAFGEARILEFGSGLRPAYPDNLPRITVRSEREIRVNGLYRHGFLIAPALAQAT
LNYVAHGQIDNEVMRCA

Sequences:

>Translated_337_residues
MQQTPTRGDRPVSIIGAGIAGAWQALLFARAGHDVTLHERSDAALMLSTSHWAGGMLAPYCESEVSEPIISRLGLASLDI
WRREMPETPFNGSLVIAHARDRADYDRFARRTSGYQRLDAAALAELEPSLEGRFREGLFYPTEGHVEPRRVLPKLHQRIV
EAGGRVLFNSNVSTDDLDGLVIDCRGLAARQAEPELRGVKGEMILIETSEVSLARPVRLMHPRFPLYLIPREDNLFMLGA
TSLEAEDTGVSLRSALELLGAAYSVHPAFGEARILEFGSGLRPAYPDNLPRITVRSEREIRVNGLYRHGFLIAPALAQAT
LNYVAHGQIDNEVMRCA
>Mature_337_residues
MQQTPTRGDRPVSIIGAGIAGAWQALLFARAGHDVTLHERSDAALMLSTSHWAGGMLAPYCESEVSEPIISRLGLASLDI
WRREMPETPFNGSLVIAHARDRADYDRFARRTSGYQRLDAAALAELEPSLEGRFREGLFYPTEGHVEPRRVLPKLHQRIV
EAGGRVLFNSNVSTDDLDGLVIDCRGLAARQAEPELRGVKGEMILIETSEVSLARPVRLMHPRFPLYLIPREDNLFMLGA
TSLEAEDTGVSLRSALELLGAAYSVHPAFGEARILEFGSGLRPAYPDNLPRITVRSEREIRVNGLYRHGFLIAPALAQAT
LNYVAHGQIDNEVMRCA

Specific function: May have amino acid oxidase activity in the biosynthesis of thiazole when cysteine gives its thiol group to be inserted into the thiazole molecule and the rest of the molecule is deaminated to give pyruvic acid and ammonia [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: NA

Molecular weight: Translated: 37214; Mature: 37214

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQTPTRGDRPVSIIGAGIAGAWQALLFARAGHDVTLHERSDAALMLSTSHWAGGMLAPY
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCHH
CESEVSEPIISRLGLASLDIWRREMPETPFNGSLVIAHARDRADYDRFARRTSGYQRLDA
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
AALAELEPSLEGRFREGLFYPTEGHVEPRRVLPKLHQRIVEAGGRVLFNSNVSTDDLDGL
HHHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
VIDCRGLAARQAEPELRGVKGEMILIETSEVSLARPVRLMHPRFPLYLIPREDNLFMLGA
EEECCCCCCCCCCCHHCCCCCCEEEEEECCCCHHCCHHHHCCCCCEEEEECCCCEEEEEC
TSLEAEDTGVSLRSALELLGAAYSVHPAFGEARILEFGSGLRPAYPDNLPRITVRSEREI
CCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEE
RVNGLYRHGFLIAPALAQATLNYVAHGQIDNEVMRCA
EEEEEEECCEEEHHHHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MQQTPTRGDRPVSIIGAGIAGAWQALLFARAGHDVTLHERSDAALMLSTSHWAGGMLAPY
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCHH
CESEVSEPIISRLGLASLDIWRREMPETPFNGSLVIAHARDRADYDRFARRTSGYQRLDA
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
AALAELEPSLEGRFREGLFYPTEGHVEPRRVLPKLHQRIVEAGGRVLFNSNVSTDDLDGL
HHHHHCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
VIDCRGLAARQAEPELRGVKGEMILIETSEVSLARPVRLMHPRFPLYLIPREDNLFMLGA
EEECCCCCCCCCCCHHCCCCCCEEEEEECCCCHHCCHHHHCCCCCEEEEECCCCEEEEEC
TSLEAEDTGVSLRSALELLGAAYSVHPAFGEARILEFGSGLRPAYPDNLPRITVRSEREI
CCCCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCEE
RVNGLYRHGFLIAPALAQATLNYVAHGQIDNEVMRCA
EEEEEEECCEEEHHHHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]