| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is murE [H]
Identifier: 146342503
GI number: 146342503
Start: 5859102
End: 5860562
Strand: Reverse
Name: murE [H]
Synonym: BRADO5664
Alternate gene names: 146342503
Gene position: 5860562-5859102 (Counterclockwise)
Preceding gene: 146342504
Following gene: 146342502
Centisome position: 78.6
GC content: 67.69
Gene sequence:
>1461_bases ATGAAACTTCACGACCTCCTCGGCGATCAGGCGGGGCTTGACCCGCGCGTCGCCGCCGTCGAAGTCTCCGGCCTTGCCGC GGACAGCCGCGCTGTCCGGCCCGGAGACCTGTTCTTCGCGCTGTCGGGAACCAAGACCGATGGCACTCGCTTCATCGCGG CTGCGGTCGCTGCGGGGGCGATCGCGGTGGCCGGCCACGAAGCCCCGGCCGGTGATCTGCCCGTTCCCTTCGTCTCGCTG CCAAATCCGCGACGTGCTCTGGCGCTTGCTGCATCGCGCGTCTTCCCCCGGCAGCCGGCAACGATCGCGGCGGTGACCGG AACCAGCGGCAAGACGTCGGTCGCAGCCTTCACGCGGCAGATCTGGCAGAGCCTCGGCCACAACGCGGCGAGTATCGGCA CGGTCGGATTGATTTCGGACAAGCGCACCGTCTACGGCTCGCTGACCACGCCCGACCCGATTGCGCTGCATCGGCAGATC GACGAGATCACCCAGGACGGCGTGACGCACCTCGCTTTCGAAGCGTCGTCGCATGGGCTCGATCAATACAGGCTGGACGG CGTCAGGGTCTCGGCCGGCGGGTTCACCAACCTGTCGCGCGACCACATGGATTACCACCCCGATCTCGTCCATTATCTCA ATGCCAAGCTGCGGCTGTTTCGCGACCTTGTGCCGCCGGGCGGGCCGGCGGTGATTTCGGCGGATCATGAGTGCTCGGCA GAAGTGATTGCCGCGGCTGATGCGCGCGGGCTCCGGCTGATGACGGTGGGGACGCATGGCAATGGCGCAGGGGCGGGCAT CCGTCTCGTGGCCGCCGCCGTCGATGGCTTTGCACAGCAGCTCGAGCTCCAGCATTGCGGCCGGATCACCTTCCTCCGCC TGCCGCTGGTCGGCGCGTTTCAGATCGAGAATGCGCTGGTGGCCGCGGGCCTTGCCATCGGCACCGGCAGCGATCCGCAG GCGGTGTTCGAGGCTCTGGAAAAGCTCGAAGGCGCCAAGGGCCGGCTCGAACTGGTCGGTGAGCACAACGGCGCGCCCAT CTTCATCGACTACGCTCACAAGCCGGATGCGCTGGCGAAGGCGCTGCACGCCTTGCGGCCGTATGCCCAGAGAAAGCTCG TGGTCGTGTTCGGCGCCGGCGGCGACCGGGACAGCGGCAAGCGGCCGCTGATGGGCGAGATCGCCGCTGCCGGCGCTGAT CGCGTTATCGTCACCGACGACAATCCCCGGAGCGAGAATCCCGCTGCGATCCGCGCCGCGATCCTGGCCGCCGCACCTGG TGCGCGCGAGATCGGCGATCGCGCCGAGGCGATCCGGGTGGCGATCAGCGAGCTTCAGCCGGGGGATGCGCTGGTCATCG CCGGCAAGGGCCACGAGACCGGACAGATCATCGGCGATACGGTTCTGCATTTCAGCGATCACGAAGCGGTCGCGGACGCA CTGGCAACGAGGGCATCATGA
Upstream 100 bases:
>100_bases TTCTTGCGGCATCGCGGCTGACGCAGTAAGCCGTCCGCGAATCGCATGGCTTTGGAGCCGCCGCCGCCCGGGGCGGGGCC AGGACCGGTACGGGACCAGG
Downstream 100 bases:
>100_bases CGAAGGCGCTGTGGACGGTGGCCGAGGCGGCGCGCGCGCTCGGTCTCGCCGGCGACTATCCTGAGACCGCGATCGATTTC GTCACCCAGGACAGCCGCGC
Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Products: NA
Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]
Number of amino acids: Translated: 486; Mature: 486
Protein sequence:
>486_residues MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA LATRAS
Sequences:
>Translated_486_residues MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA LATRAS >Mature_486_residues MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA LATRAS
Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]
COG id: COG0769
COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family. MurE subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=36.7556468172485, Blast_Score=230, Evalue=2e-61,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005761 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.13 [H]
Molecular weight: Translated: 50452; Mature: 50452
Theoretical pI: Translated: 6.61; Mature: 6.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 0.8 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 0.8 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGA CCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCE IAVAGHEAPAGDLPVPFVSLPNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQ EEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH IWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQIDEITQDGVTHLAFEASSHGL HHHHHCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC DQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA CCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAF CEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE QIENALVAAGLAIGTGSDPQAVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAK EHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH ALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGADRVIVTDDNPRSENPAAIRAA HHHHHCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHH ILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCEEEEECCHHHHHHH LATRAS HHHCCC >Mature Secondary Structure MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGA CCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCE IAVAGHEAPAGDLPVPFVSLPNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQ EEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH IWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQIDEITQDGVTHLAFEASSHGL HHHHHCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC DQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA CCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAF CEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE QIENALVAAGLAIGTGSDPQAVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAK EHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH ALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGADRVIVTDDNPRSENPAAIRAA HHHHHCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHH ILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCEEEEECCHHHHHHH LATRAS HHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12597275 [H]