Click here to switch to the map view.

The map label for this gene is ddlB [H]

Identifier: 146342495

GI number: 146342495

Start: 5848920

End: 5849909

Strand: Reverse

Name: ddlB [H]

Synonym: BRADO5656

Alternate gene names: 146342495

Gene position: 5849909-5848920 (Counterclockwise)

Preceding gene: 146342496

Following gene: 146342494

Centisome position: 78.45

GC content: 67.47

Gene sequence:

>990_bases
ATGCGCACCACGATTCTGTTCGGCGGCTCCAACAAGGAACGTCTCGTCTCGGTCGCCAGCGCCAAGGCGCTGCACGCCGC
GCTGCCGGAGGCGGACCTCTGGTTCTGGGAGGCCGACGACACTGTGCAGCAGGCAAGCGGCCCAGCGCTGGTCGCCCACG
CAAAGCCGTTCGAGGAGCCGTTCACGGCCGACGGCGTCCGGATCGGCACGCTCGCCGAGGCGCTCGATCGTGCCCGCGCC
GAGGACCGGATCCTGGTTCTCGGACTTCATGGCGGACGGGCGGAGAACGGCGAGCTTCAAGTGATGTGCGAGATGCGGGG
CGTGCCGTTCACCGGCTCGAGCTCGGCGGCCTCGCACCTCGCCTTCGACAAGGTCGCCGCCAAGCGCTTCGCCGTACTCG
GTGGCGTCACGGCGGCCGAAGGTGTCGCGCTCGACAATGCCGAGACAGCTCTCGCGCGTTACGGCAAGCTGATCGCCAAG
CCGGTGAAGGATGGCTCGAGCTTCGGCCTGATCTTCGTCAACGCCAAACAGGACCTCGTCGCCGTTAAGGCCGCCGCTGC
GACCGAGGAGTATCTCATCGAGCCGTTCGTCACCGGGAAGGAGGCGACCTGCGGCGTGCTCGAGCAGGCGGACGGCTCGT
TGATCGCGCTGCCGCCGATCGAGATCCTGCCGGCCGACGGCGCCTTCAACTACTTCGCGAAGTACCTGGCGGCCGCGACG
AAGGAGATCTGTCCGGGACGCTTCAATCCGGAGGTTACGGCAGCGCTGCAGCGAGAGGCGATCAAGGCGCATCGTGCGCT
GTCCTGCGGCGGCTACTCGCGCACCGACTTCATCGTCTCGGCCGACGGCCCGGTCTATCTCGAGACCAACACGCTGCCCG
GCTTGACCGCCGCCTCGCTATATCCGAAGGCGCTCGCGGCGCAGGGTATCCCCTTTGCCGACTTTCTGCAGCAGCAGATC
GCCTTGGCGGAACGCCGCGTTCGGCACTGA

Upstream 100 bases:

>100_bases
AGATCAAGCGGATCGGAGTCAAGGCGAGCTGACGACCCGCTTCTTCACGTGACGTCCACGCTGTCGCAACCGCATTCTGA
GATCAATCGAGAAACCATTG

Downstream 100 bases:

>100_bases
ATTCGTGCGGTAGTCGCACCGGAGGAACCCGGCCCGGCGTCCCCCGGAGGTTCCTGTAACCCGTAAAATGCTTAAGAATT
CCGGGCAAGGTCCTCATAAA

Product: D-alanine-D-alanine ligase, affects cell division

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRTTILFGGSNKERLVSVASAKALHAALPEADLWFWEADDTVQQASGPALVAHAKPFEEPFTADGVRIGTLAEALDRARA
EDRILVLGLHGGRAENGELQVMCEMRGVPFTGSSSAASHLAFDKVAAKRFAVLGGVTAAEGVALDNAETALARYGKLIAK
PVKDGSSFGLIFVNAKQDLVAVKAAAATEEYLIEPFVTGKEATCGVLEQADGSLIALPPIEILPADGAFNYFAKYLAAAT
KEICPGRFNPEVTAALQREAIKAHRALSCGGYSRTDFIVSADGPVYLETNTLPGLTAASLYPKALAAQGIPFADFLQQQI
ALAERRVRH

Sequences:

>Translated_329_residues
MRTTILFGGSNKERLVSVASAKALHAALPEADLWFWEADDTVQQASGPALVAHAKPFEEPFTADGVRIGTLAEALDRARA
EDRILVLGLHGGRAENGELQVMCEMRGVPFTGSSSAASHLAFDKVAAKRFAVLGGVTAAEGVALDNAETALARYGKLIAK
PVKDGSSFGLIFVNAKQDLVAVKAAAATEEYLIEPFVTGKEATCGVLEQADGSLIALPPIEILPADGAFNYFAKYLAAAT
KEICPGRFNPEVTAALQREAIKAHRALSCGGYSRTDFIVSADGPVYLETNTLPGLTAASLYPKALAAQGIPFADFLQQQI
ALAERRVRH
>Mature_329_residues
MRTTILFGGSNKERLVSVASAKALHAALPEADLWFWEADDTVQQASGPALVAHAKPFEEPFTADGVRIGTLAEALDRARA
EDRILVLGLHGGRAENGELQVMCEMRGVPFTGSSSAASHLAFDKVAAKRFAVLGGVTAAEGVALDNAETALARYGKLIAK
PVKDGSSFGLIFVNAKQDLVAVKAAAATEEYLIEPFVTGKEATCGVLEQADGSLIALPPIEILPADGAFNYFAKYLAAAT
KEICPGRFNPEVTAALQREAIKAHRALSCGGYSRTDFIVSADGPVYLETNTLPGLTAASLYPKALAAQGIPFADFLQQQI
ALAERRVRH

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=338, Percent_Identity=32.2485207100592, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=26.7409470752089, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34796; Mature: 34796

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTILFGGSNKERLVSVASAKALHAALPEADLWFWEADDTVQQASGPALVAHAKPFEEP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCEEEEECCCCCCC
FTADGVRIGTLAEALDRARAEDRILVLGLHGGRAENGELQVMCEMRGVPFTGSSSAASHL
CCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
AFDKVAAKRFAVLGGVTAAEGVALDNAETALARYGKLIAKPVKDGSSFGLIFVNAKQDLV
HHHHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
AVKAAAATEEYLIEPFVTGKEATCGVLEQADGSLIALPPIEILPADGAFNYFAKYLAAAT
EHHHHHCCHHHHHCCCCCCCCCCCHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHHHH
KEICPGRFNPEVTAALQREAIKAHRALSCGGYSRTDFIVSADGPVYLETNTLPGLTAASL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHH
YPKALAAQGIPFADFLQQQIALAERRVRH
CHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTTILFGGSNKERLVSVASAKALHAALPEADLWFWEADDTVQQASGPALVAHAKPFEEP
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCEEEEECCCCCCC
FTADGVRIGTLAEALDRARAEDRILVLGLHGGRAENGELQVMCEMRGVPFTGSSSAASHL
CCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
AFDKVAAKRFAVLGGVTAAEGVALDNAETALARYGKLIAKPVKDGSSFGLIFVNAKQDLV
HHHHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
AVKAAAATEEYLIEPFVTGKEATCGVLEQADGSLIALPPIEILPADGAFNYFAKYLAAAT
EHHHHHCCHHHHHCCCCCCCCCCCHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHHHH
KEICPGRFNPEVTAALQREAIKAHRALSCGGYSRTDFIVSADGPVYLETNTLPGLTAASL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHH
YPKALAAQGIPFADFLQQQIALAERRVRH
CHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA