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The map label for this gene is livK [C]

Identifier: 146342438

GI number: 146342438

Start: 5780755

End: 5781894

Strand: Reverse

Name: livK [C]

Synonym: BRADO5597

Alternate gene names: 146342438

Gene position: 5781894-5780755 (Counterclockwise)

Preceding gene: 146342439

Following gene: 146342437

Centisome position: 77.54

GC content: 64.39

Gene sequence:

>1140_bases
GTGAGATCGATCATCCTGGCCGGACTGTGCTCCGCCGTACTGCTGTCAGGAGCCGCCTACGCGCAATCCGGCGCCCCCAT
CAAGATCGCAAACGTCGCCGAGCTTTCCGGCGGCGGCGCGACCGTCGGTACCAACTGGAAGAACGGCATCGACCTCGCCG
TCGAGGAAATCAACGCCAAGGGCGGCATCCTCGGCCGCAAGATCGAGGTCAGCCACGCCGACTCGCAGTCGAACCCCGGC
GTCGCCCGCGCGCAGGTGCAGAAAGCGCTCGACAGCGAGCCCTACGTGCTGCTCGGGCCGGGCTATTCCGGCTCGGTGAA
GGTGACCGCGCCGCTGGCCGCTGAAGCCGGCATCACCCAGATCATGGGCGGCGAGGCGGCCGAGCTGACGCAGGCCGGCA
ACAAGTTCCTGTTCCGCACTTCGTTCGGCCAGCAGTCCTCGATGCCGAAGGTCGCCAAGTACATCAACGATGAGCTGAAG
GCGAAGTCGGTCGCGGTGGTCTGGGTCAACAACGATTTCGGCCGCGGCGGGCGCGACGTCATCACCAAGGAGTTCAACCG
TCTCGGCATCAAGGTCGCCGCGGACCTCTCGACCGAAGCGGGCCAGGCGGACTTCGCCGCCGACGTCAGCAAGATCAAGG
CGGCGGCACCTGATGCCGTCTTCATCTACGTCAACGAGGAAGAGAGCGCGCGCATCCTGAAAGAGATCAAGCGCCAGGGC
GTGACCGCGCCGCTGATCGGCGAGACCACGCTGGTCGGCCAGAAGGTGATCGAACTCGCCGGCGATGCCGCCAACGGCGC
CCGCGGCCACGTCGGCCTCACGACCGATGCGCCGGTCGAGGCTATCAAGGCGTTCCGCGACCGCTTCGTGAAGAAGTACA
ACTACGTGCCCGACCATAACGGCCTGAAGGGCTACCTCGCCATCTACATGGTCAAGGCCACGACCGAGAAAATGGGCAAG
GTCGATTCCAAGGGCTTTGCCGATGCATTGCACGGCTTCACCATCAAGACCTCGGCCGAGCCGAACATCCTGATGGATGT
CACCTTCGACCAGAACGGCGACATCGATCGCCAGGGCTTCCTGGTCGAGGTCGTCGAAGGCAAGCAGGTCGTCAAGCAGG
TGCTGCCAAAGCTGAACTAA

Upstream 100 bases:

>100_bases
CAGGCGGTTGACGCCGCCTGAGCCGACCGCCAATACGCGGCGGAACACATCGTTCAAGCAAGAAACGCAAACAAGCGTTT
AGAGCAGAGGAGGAAACAAC

Downstream 100 bases:

>100_bases
CGAGACCGGTCGATCTGCGGCGCTGGAGGGGGACTGTCCAGCGTCGCCGTCACCCAAGGGCTCACGGGGAAACGATGTCC
AATCTGCTCGACCTGATCGT

Product: branched chain amino-acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRSIILAGLCSAVLLSGAAYAQSGAPIKIANVAELSGGGATVGTNWKNGIDLAVEEINAKGGILGRKIEVSHADSQSNPG
VARAQVQKALDSEPYVLLGPGYSGSVKVTAPLAAEAGITQIMGGEAAELTQAGNKFLFRTSFGQQSSMPKVAKYINDELK
AKSVAVVWVNNDFGRGGRDVITKEFNRLGIKVAADLSTEAGQADFAADVSKIKAAAPDAVFIYVNEEESARILKEIKRQG
VTAPLIGETTLVGQKVIELAGDAANGARGHVGLTTDAPVEAIKAFRDRFVKKYNYVPDHNGLKGYLAIYMVKATTEKMGK
VDSKGFADALHGFTIKTSAEPNILMDVTFDQNGDIDRQGFLVEVVEGKQVVKQVLPKLN

Sequences:

>Translated_379_residues
MRSIILAGLCSAVLLSGAAYAQSGAPIKIANVAELSGGGATVGTNWKNGIDLAVEEINAKGGILGRKIEVSHADSQSNPG
VARAQVQKALDSEPYVLLGPGYSGSVKVTAPLAAEAGITQIMGGEAAELTQAGNKFLFRTSFGQQSSMPKVAKYINDELK
AKSVAVVWVNNDFGRGGRDVITKEFNRLGIKVAADLSTEAGQADFAADVSKIKAAAPDAVFIYVNEEESARILKEIKRQG
VTAPLIGETTLVGQKVIELAGDAANGARGHVGLTTDAPVEAIKAFRDRFVKKYNYVPDHNGLKGYLAIYMVKATTEKMGK
VDSKGFADALHGFTIKTSAEPNILMDVTFDQNGDIDRQGFLVEVVEGKQVVKQVLPKLN
>Mature_379_residues
MRSIILAGLCSAVLLSGAAYAQSGAPIKIANVAELSGGGATVGTNWKNGIDLAVEEINAKGGILGRKIEVSHADSQSNPG
VARAQVQKALDSEPYVLLGPGYSGSVKVTAPLAAEAGITQIMGGEAAELTQAGNKFLFRTSFGQQSSMPKVAKYINDELK
AKSVAVVWVNNDFGRGGRDVITKEFNRLGIKVAADLSTEAGQADFAADVSKIKAAAPDAVFIYVNEEESARILKEIKRQG
VTAPLIGETTLVGQKVIELAGDAANGARGHVGLTTDAPVEAIKAFRDRFVKKYNYVPDHNGLKGYLAIYMVKATTEKMGK
VDSKGFADALHGFTIKTSAEPNILMDVTFDQNGDIDRQGFLVEVVEGKQVVKQVLPKLN

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=263, Percent_Identity=28.1368821292776, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789867, Length=263, Percent_Identity=28.5171102661597, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40036; Mature: 40036

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIILAGLCSAVLLSGAAYAQSGAPIKIANVAELSGGGATVGTNWKNGIDLAVEEINAK
CCHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCEECCCCCCCHHHEEEECCCC
GGILGRKIEVSHADSQSNPGVARAQVQKALDSEPYVLLGPGYSGSVKVTAPLAAEAGITQ
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHH
IMGGEAAELTQAGNKFLFRTSFGQQSSMPKVAKYINDELKAKSVAVVWVNNDFGRGGRDV
HCCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHH
ITKEFNRLGIKVAADLSTEAGQADFAADVSKIKAAAPDAVFIYVNEEESARILKEIKRQG
HHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCC
VTAPLIGETTLVGQKVIELAGDAANGARGHVGLTTDAPVEAIKAFRDRFVKKYNYVPDHN
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
GLKGYLAIYMVKATTEKMGKVDSKGFADALHGFTIKTSAEPNILMDVTFDQNGDIDRQGF
CCCEEEEEEEEEHHHHHHCCCCCCCHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCE
LVEVVEGKQVVKQVLPKLN
EEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MRSIILAGLCSAVLLSGAAYAQSGAPIKIANVAELSGGGATVGTNWKNGIDLAVEEINAK
CCHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCEECCCCCCCHHHEEEECCCC
GGILGRKIEVSHADSQSNPGVARAQVQKALDSEPYVLLGPGYSGSVKVTAPLAAEAGITQ
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHH
IMGGEAAELTQAGNKFLFRTSFGQQSSMPKVAKYINDELKAKSVAVVWVNNDFGRGGRDV
HCCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHH
ITKEFNRLGIKVAADLSTEAGQADFAADVSKIKAAAPDAVFIYVNEEESARILKEIKRQG
HHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCC
VTAPLIGETTLVGQKVIELAGDAANGARGHVGLTTDAPVEAIKAFRDRFVKKYNYVPDHN
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
GLKGYLAIYMVKATTEKMGKVDSKGFADALHGFTIKTSAEPNILMDVTFDQNGDIDRQGF
CCCEEEEEEEEEHHHHHHCCCCCCCHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCCE
LVEVVEGKQVVKQVLPKLN
EEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA