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The map label for this gene is folA [H]

Identifier: 146342417

GI number: 146342417

Start: 5760730

End: 5761242

Strand: Reverse

Name: folA [H]

Synonym: BRADO5576

Alternate gene names: 146342417

Gene position: 5761242-5760730 (Counterclockwise)

Preceding gene: 146342418

Following gene: 146342416

Centisome position: 77.26

GC content: 68.23

Gene sequence:

>513_bases
ATGATCGAGATCGTGCAGATCGTCGCGGTCGCCGAGAACGGCGTGATCGGCGCTGCCGGCGCCATTCCCTGGCGGCAGAA
GAGCGACATGCAGCGCTTCAAGGCGCTGACCATGGGACGGCCGATCGTCATGGGCCGCAAGACCTTCGTGTCATTTCCTC
GCCGGCCGTTGCCGGGTCGGACCAACATCATCGTCACCCGCGACGCCGCGTTTCGCGCCGCGGGCGCCATCGTGACTCAC
CGCATCGCCGAAGCGCTCACCGTCGCGCGGGGCGATGCGCTGCGGCGTTCGGCCGCGGAAATCGCCATCGTCGGCGGCGC
CGAGATCTATACGGCGACCCTGCCATTGACCGACAGATTGGAGATCACCGAGGTCCACGCGCGGCCGCCGGGCGACACGC
TGTTTCCGGCGATCGATCCGGCGCAGTGGGAGGAGGTGGCGCGCGAGCGTCACGCGTCGGGGCCGGACGATCAGGCCGAC
TTCTCTTACGTCACCTATCGCCGGAAGCGCTGA

Upstream 100 bases:

>100_bases
CGATCACCTTCTACAAGTCGCTCGGCGCCGAGCTGATGGACGACTGGAAGCTCTGCAAGGTCACCGGGCCCGCGCTGACG
CAACTGGCCGAAAGAGCCCC

Downstream 100 bases:

>100_bases
CCGGCACCTGCCCGCAAAGTCCCTGCCGCCCCTTCAAGACCATCCGTTAATGATGAAGTCGCGTTGTTTGGCCGCGGTGG
CCCTGTCCAGCCGCGGCAAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MIEIVQIVAVAENGVIGAAGAIPWRQKSDMQRFKALTMGRPIVMGRKTFVSFPRRPLPGRTNIIVTRDAAFRAAGAIVTH
RIAEALTVARGDALRRSAAEIAIVGGAEIYTATLPLTDRLEITEVHARPPGDTLFPAIDPAQWEEVARERHASGPDDQAD
FSYVTYRRKR

Sequences:

>Translated_170_residues
MIEIVQIVAVAENGVIGAAGAIPWRQKSDMQRFKALTMGRPIVMGRKTFVSFPRRPLPGRTNIIVTRDAAFRAAGAIVTH
RIAEALTVARGDALRRSAAEIAIVGGAEIYTATLPLTDRLEITEVHARPPGDTLFPAIDPAQWEEVARERHASGPDDQAD
FSYVTYRRKR
>Mature_170_residues
MIEIVQIVAVAENGVIGAAGAIPWRQKSDMQRFKALTMGRPIVMGRKTFVSFPRRPLPGRTNIIVTRDAAFRAAGAIVTH
RIAEALTVARGDALRRSAAEIAIVGGAEIYTATLPLTDRLEITEVHARPPGDTLFPAIDPAQWEEVARERHASGPDDQAD
FSYVTYRRKR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=166, Percent_Identity=32.5301204819277, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17505899, Length=178, Percent_Identity=30.3370786516854, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=169, Percent_Identity=31.9526627218935, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116008026, Length=160, Percent_Identity=34.375, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647458, Length=160, Percent_Identity=34.375, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18673; Mature: 18673

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIVQIVAVAENGVIGAAGAIPWRQKSDMQRFKALTMGRPIVMGRKTFVSFPRRPLPGR
CCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCC
TNIIVTRDAAFRAAGAIVTHRIAEALTVARGDALRRSAAEIAIVGGAEIYTATLPLTDRL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEECCEEEEEECCCCCCE
EITEVHARPPGDTLFPAIDPAQWEEVARERHASGPDDQADFSYVTYRRKR
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MIEIVQIVAVAENGVIGAAGAIPWRQKSDMQRFKALTMGRPIVMGRKTFVSFPRRPLPGR
CCEEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCC
TNIIVTRDAAFRAAGAIVTHRIAEALTVARGDALRRSAAEIAIVGGAEIYTATLPLTDRL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEECCEEEEEECCCCCCE
EITEVHARPPGDTLFPAIDPAQWEEVARERHASGPDDQADFSYVTYRRKR
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]