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The map label for this gene is braE [H]

Identifier: 146342355

GI number: 146342355

Start: 5693165

End: 5694133

Strand: Reverse

Name: braE [H]

Synonym: BRADO5511

Alternate gene names: 146342355

Gene position: 5694133-5693165 (Counterclockwise)

Preceding gene: 146342356

Following gene: 146342354

Centisome position: 76.36

GC content: 65.12

Gene sequence:

>969_bases
ATGACCAGGCCGCGTCTCACCACGCTCGCCGTAATCGTCGTGGCGCTCGCCGTGGTCCCGTTCCTCGGCCTGTCCGGCGC
GCTACTGAATCTCATGATCTTCATGATGATAACAGCGCTGGCGGCGCAAGGCTGGAACATCCTCGGCGGCTATGCCGGCC
TGTCGTCGTTCGGCCACGCCGCGTTCTTCGGCGCCGGCGCCTATGCGATGGCCGTGCTGCAGACCCGCTTCGGCGTCAAC
GCCTGGATCGCGCTGGTCATCGGTATCGCGTTGGGCGCGCTGGTCGGCGCTGCCATCGGCTTTCTCAGTTTCCGCTCCGG
CCTGAAAGGCTCGTACTTTGCGCTGATCACCTTGGCCTTCGCCGAGGTCGCGCGCATCCTCGCCAACAGCACCAATTTCA
CCGGCGGCGCCGCCGGCATCCTGCTGAAGCTGCAAACGGGTCTGCCGTACCTGCAATTCGCCGACCGCCGCTATTTCCTG
CTGATCGCGCTCGGCTTCGTCGCGATCGGTCTGCTGGTGAGCTGGTGGCTGGAGCATTCGCGGTTCGGCGCCTATCTCGT
GGCGCTGCGCGAGAACGAGCAGGCAGCGCAGGCGCTCGGCGTCGATGTGTTCGCGGTCAAGATGAAGGCGATCGCGATCT
CCGGCGCGCTGACAGCTGCCGCCGGCTGCCTCTATGCGCAGAACTATCTGTTCATCGATGCCAATGTCGCGTTCGGCTCG
TGGATCTCGATCGAGGCGCTGTTCGCGGCGATCGTCGGCGGCTCCGGCACCGTGCTAGGGCCCATGCTGGGTGCGATCGT
GCTGCTGGGACTTGGCGAACTGACCAAGGGCCTCTCCGGCGGCATTCCCGGGATGGATCTCATGGTGTTCGGCGTCATCC
TCGTGCTGTCCGTGGCGTTCTCGCCGAATGGCCTGGTGATGCTGATGAAGAGCCTGGGCTTGTCCACGGCAAGAGCGAAG
GAGGCGTGA

Upstream 100 bases:

>100_bases
CCAGCCTGTTCCTCGGTGACAGCCTCGGTCAGATCGGTATCTTCCTGATTTTCATCGTCACTCTGCTGGTGCGCCCGACC
GGCCTGTTCGGAGCGCGCTC

Downstream 100 bases:

>100_bases
TGCTCGAGGTCAAGTCCCTCACCAAACGCTTCTCCGGCCTCGTCGCGGTCGACCAGGCGTCGCTGTCGGTCGCCAAAGGA
TCAATCACGGGCCTAATCGG

Product: putative branched-chain amino acid ABC transporter permease/livM-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTRPRLTTLAVIVVALAVVPFLGLSGALLNLMIFMMITALAAQGWNILGGYAGLSSFGHAAFFGAGAYAMAVLQTRFGVN
AWIALVIGIALGALVGAAIGFLSFRSGLKGSYFALITLAFAEVARILANSTNFTGGAAGILLKLQTGLPYLQFADRRYFL
LIALGFVAIGLLVSWWLEHSRFGAYLVALRENEQAAQALGVDVFAVKMKAIAISGALTAAAGCLYAQNYLFIDANVAFGS
WISIEALFAAIVGGSGTVLGPMLGAIVLLGLGELTKGLSGGIPGMDLMVFGVILVLSVAFSPNGLVMLMKSLGLSTARAK
EA

Sequences:

>Translated_322_residues
MTRPRLTTLAVIVVALAVVPFLGLSGALLNLMIFMMITALAAQGWNILGGYAGLSSFGHAAFFGAGAYAMAVLQTRFGVN
AWIALVIGIALGALVGAAIGFLSFRSGLKGSYFALITLAFAEVARILANSTNFTGGAAGILLKLQTGLPYLQFADRRYFL
LIALGFVAIGLLVSWWLEHSRFGAYLVALRENEQAAQALGVDVFAVKMKAIAISGALTAAAGCLYAQNYLFIDANVAFGS
WISIEALFAAIVGGSGTVLGPMLGAIVLLGLGELTKGLSGGIPGMDLMVFGVILVLSVAFSPNGLVMLMKSLGLSTARAK
EA
>Mature_321_residues
TRPRLTTLAVIVVALAVVPFLGLSGALLNLMIFMMITALAAQGWNILGGYAGLSSFGHAAFFGAGAYAMAVLQTRFGVNA
WIALVIGIALGALVGAAIGFLSFRSGLKGSYFALITLAFAEVARILANSTNFTGGAAGILLKLQTGLPYLQFADRRYFLL
IALGFVAIGLLVSWWLEHSRFGAYLVALRENEQAAQALGVDVFAVKMKAIAISGALTAAAGCLYAQNYLFIDANVAFGSW
ISIEALFAAIVGGSGTVLGPMLGAIVLLGLGELTKGLSGGIPGMDLMVFGVILVLSVAFSPNGLVMLMKSLGLSTARAKE
A

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=329, Percent_Identity=28.8753799392097, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33336; Mature: 33205

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPRLTTLAVIVVALAVVPFLGLSGALLNLMIFMMITALAAQGWNILGGYAGLSSFGHA
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
AFFGAGAYAMAVLQTRFGVNAWIALVIGIALGALVGAAIGFLSFRSGLKGSYFALITLAF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AEVARILANSTNFTGGAAGILLKLQTGLPYLQFADRRYFLLIALGFVAIGLLVSWWLEHS
HHHHHHHHCCCCCCCCHHHEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
RFGAYLVALRENEQAAQALGVDVFAVKMKAIAISGALTAAAGCLYAQNYLFIDANVAFGS
CCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
WISIEALFAAIVGGSGTVLGPMLGAIVLLGLGELTKGLSGGIPGMDLMVFGVILVLSVAF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
SPNGLVMLMKSLGLSTARAKEA
CCCHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TRPRLTTLAVIVVALAVVPFLGLSGALLNLMIFMMITALAAQGWNILGGYAGLSSFGHA
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCH
AFFGAGAYAMAVLQTRFGVNAWIALVIGIALGALVGAAIGFLSFRSGLKGSYFALITLAF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AEVARILANSTNFTGGAAGILLKLQTGLPYLQFADRRYFLLIALGFVAIGLLVSWWLEHS
HHHHHHHHCCCCCCCCHHHEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
RFGAYLVALRENEQAAQALGVDVFAVKMKAIAISGALTAAAGCLYAQNYLFIDANVAFGS
CCCEEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
WISIEALFAAIVGGSGTVLGPMLGAIVLLGLGELTKGLSGGIPGMDLMVFGVILVLSVAF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
SPNGLVMLMKSLGLSTARAKEA
CCCHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]