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The map label for this gene is fixA [H]

Identifier: 146342234

GI number: 146342234

Start: 5592364

End: 5593209

Strand: Reverse

Name: fixA [H]

Synonym: BRADO5386

Alternate gene names: 146342234

Gene position: 5593209-5592364 (Counterclockwise)

Preceding gene: 146342235

Following gene: 146342233

Centisome position: 75.01

GC content: 66.55

Gene sequence:

>846_bases
ATGCATATCGTCGTCTGCATCAAGCAGGTCCCGGACTCCGCGCAGATCCGCGTCCACCCGGTGACCAACACCATCATGCG
CCAGGGTGTGCCGACCATCATCAATCCCTACGACCTGTTCGCGCTCGAAGCCGCGTTGCAGCTGCGTGACAAGTTCGGCG
GCGAAGTGACCGTGCTGACGATGGGACCGCCGTCCGCCGAGGATTCGCTGCGCCGCGCGCTGACCTACGGCGCCGATCGC
GCGGTGCTGCTGACCGACCGCTTCTTCGCCGGCGCCGACACGCTGGCGACGACCTATGCGCTCGCGACCGCCATCCGCAA
GATCAGCGAGACCTTTGGTGCGCCGGATATCGTGTTCACCGGCAAGCAGACGATCGATGGCGACACCGCCCAGGTCGGTC
CCGGCATCGCCAAGCGGCTCGGCCTGCTGCAGCTGACCTATGTGGCCAAGATCAACGAGGTCGATCCGGCCGCGCGCACC
ATCGAGGCGGAGCGCCGCTCGGAAGGTGGCGTTCAGGTGCTCAAGACCAGGCTGCCGTGCCTGGTCACGATGCTCGAGGC
GACCAATGAGATCCGCCGCGGCGGCATGGCGGATGCGCTGCGTGCCGCGCGCGCGCAGATCGTGACCTGGAGCGCCAAGG
ACGCCGGCGTCGAGGACATCTCCAAATGCGGCCTGAAGGGCTCGCCCACGATCGTCAAGCGGGTGTTCGCACCTTCGGCG
CGCGCCGAAAAGGCGGTCTTCGTCGATTTCAGCAATAAGCCGCCGGCGGAGGCGCTGATCGAGGAATTGTTCAAGCGGCA
GCCGAAGCTCGAGGCCGAGCTGATCTCGTCGGCGCACGGCTACTGA

Upstream 100 bases:

>100_bases
GCCTGGCGCAAGGCGTTGAATCGACGATGATAATCATCGTGCTCGAAACTGGTACGACAATTGCTGACCTCATCGCAGGC
ACACACACCGGAGAGATCGC

Downstream 100 bases:

>100_bases
CTTCGCCATCTCACGACGGTTGAGACTTCCAGCTTCTTTCGGATCAACGGGACGACGACCATGAGCGAACCCACCAAAGC
ACCGCCCGCCGCAGCCGGTC

Product: protein fixA/electron transfer flavoprotein beta-subunit (beta-ETF)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALQLRDKFGGEVTVLTMGPPSAEDSLRRALTYGADR
AVLLTDRFFAGADTLATTYALATAIRKISETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKINEVDPAART
IEAERRSEGGVQVLKTRLPCLVTMLEATNEIRRGGMADALRAARAQIVTWSAKDAGVEDISKCGLKGSPTIVKRVFAPSA
RAEKAVFVDFSNKPPAEALIEELFKRQPKLEAELISSAHGY

Sequences:

>Translated_281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALQLRDKFGGEVTVLTMGPPSAEDSLRRALTYGADR
AVLLTDRFFAGADTLATTYALATAIRKISETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKINEVDPAART
IEAERRSEGGVQVLKTRLPCLVTMLEATNEIRRGGMADALRAARAQIVTWSAKDAGVEDISKCGLKGSPTIVKRVFAPSA
RAEKAVFVDFSNKPPAEALIEELFKRQPKLEAELISSAHGY
>Mature_281_residues
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALQLRDKFGGEVTVLTMGPPSAEDSLRRALTYGADR
AVLLTDRFFAGADTLATTYALATAIRKISETFGAPDIVFTGKQTIDGDTAQVGPGIAKRLGLLQLTYVAKINEVDPAART
IEAERRSEGGVQVLKTRLPCLVTMLEATNEIRRGGMADALRAARAQIVTWSAKDAGVEDISKCGLKGSPTIVKRVFAPSA
RAEKAVFVDFSNKPPAEALIEELFKRQPKLEAELISSAHGY

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI4503609, Length=225, Percent_Identity=31.1111111111111, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI62420877, Length=212, Percent_Identity=31.1320754716981, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI87081682, Length=219, Percent_Identity=29.2237442922374, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367123, Length=216, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141345, Length=224, Percent_Identity=33.9285714285714, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321646, Length=211, Percent_Identity=27.4881516587678, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24651147, Length=218, Percent_Identity=31.1926605504587, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651145, Length=218, Percent_Identity=31.1926605504587, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 30345; Mature: 30345

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01065 ETF_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALQLRDKFGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEEE
MGPPSAEDSLRRALTYGADRAVLLTDRFFAGADTLATTYALATAIRKISETFGAPDIVFT
ECCCCHHHHHHHHHHCCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GKQTIDGDTAQVGPGIAKRLGLLQLTYVAKINEVDPAARTIEAERRSEGGVQVLKTRLPC
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
LVTMLEATNEIRRGGMADALRAARAQIVTWSAKDAGVEDISKCGLKGSPTIVKRVFAPSA
HHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCC
RAEKAVFVDFSNKPPAEALIEELFKRQPKLEAELISSAHGY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPTIINPYDLFALEAALQLRDKFGGEVTVLT
CEEEEEEEECCCCCCEEEEECHHHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCEEEEEE
MGPPSAEDSLRRALTYGADRAVLLTDRFFAGADTLATTYALATAIRKISETFGAPDIVFT
ECCCCHHHHHHHHHHCCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GKQTIDGDTAQVGPGIAKRLGLLQLTYVAKINEVDPAARTIEAERRSEGGVQVLKTRLPC
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
LVTMLEATNEIRRGGMADALRAARAQIVTWSAKDAGVEDISKCGLKGSPTIVKRVFAPSA
HHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCHHHHHHHHCCCC
RAEKAVFVDFSNKPPAEALIEELFKRQPKLEAELISSAHGY
CCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088; 3216855 [H]