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The map label for this gene is fixB [H]

Identifier: 146342233

GI number: 146342233

Start: 5591197

End: 5592303

Strand: Reverse

Name: fixB [H]

Synonym: BRADO5385

Alternate gene names: 146342233

Gene position: 5592303-5591197 (Counterclockwise)

Preceding gene: 146342234

Following gene: 146342232

Centisome position: 75.0

GC content: 66.85

Gene sequence:

>1107_bases
ATGAGCGAACCCACCAAAGCACCGCCCGCCGCAGCCGGTCGCGCCGCGACCAAGAAGGAGCTGCCGGAGCATTTCAAGGC
CTACAAGCACGTCTGGGTGTTCATCGAGCAGGAGCGCGGGCAGGTTCATCCCGTGTCCTGGGAGCTGATGGGCGCCGGCC
GCAAGCTCGCCGACAAGCTGAAGGTCGATCTTGCGGCCGTGGTCATCGGACCGCCCGGTGAGGCCGTGCAGCATGCCGCC
GCCGAGTCGTTCTGCTACGGTGCCGATCTGGCTTACATCGTGGCCGACGAGGTTCTTACCGACTATCGCAACGAGGCCTA
CACCAAGGCGCTGACCGATACCGTCAACACCTTCAAGCCGGAGATCCTGCTGCTCGGCGCCACCACGCTGGGCCGCGACC
TCGCCGGCTCGGTGGCCACCACCTTGCTGACCGGTCTCACCGCCGACTGCACCGAGCTCGACGTCGATGCCGACGGCTCG
CTGGCCGCGACGCGACCGACCTTCGGCGGTTCGCTGCTGTGCACGATCTACACGCTGAACTACCGGCCGCAGATGGCGAC
CGTGCGCCCGCGCGTGATGCCGATGCCCGAACGGGTCGAGAAGCCGGCGACCCGGATCATCGAGCACAAGCTCGGCCTGG
TCGAGGAGGACATCGTCACCAAAGTGCTGTCCTTCATTCCGGACCGCGACTCCGCCAAGTCCAATCTCGCTTATGCCGAC
ATCGTCGTCGCCGGTGGGCTCGGACTGGGCTCGCCCGAGAACTTCCAGCTCGTCAAGGGCCTGGCGGCGGTGCTCGGCGC
GGAGTTCGGCTGCTCGCGCCCGCTGGTACAGAAGGGCTGGGTGACTTCGGACCGCCAGATCGGCCAGACCGGCAAGACGA
TCCGTCCGAAGCTGTACATCGCCGCCGGCATCTCCGGCGCGATCCAGCATCGGGTGGGCGTCGAGGGCGCCGATCTGATC
GTCGCCATCAACACCGACAAGAACGCGCCGATCTTCGATTTCGCGCATATCGGCATCGTCACCGACGCGATCCGGCTGCT
GCCGGCGCTCACCGAAGCATTCCGCGCGCGGCTGTCGCCGCATTCGCGCGACCGCATCGCAAGCTAG

Upstream 100 bases:

>100_bases
GCTCGAGGCCGAGCTGATCTCGTCGGCGCACGGCTACTGACTTCGCCATCTCACGACGGTTGAGACTTCCAGCTTCTTTC
GGATCAACGGGACGACGACC

Downstream 100 bases:

>100_bases
GCACCAGAGGAGCAGGGCAATGATCGAGGAACGTTTCGACGCGATCGTCGTGGGCGCCGGCATGGCCGGCAATGCGGCTG
CGCTGACCATGGCCCAGCGC

Product: protein FixB/electron transfer flavoprotein subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSEPTKAPPAAAGRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVDLAAVVIGPPGEAVQHAA
AESFCYGADLAYIVADEVLTDYRNEAYTKALTDTVNTFKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGS
LAATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPERVEKPATRIIEHKLGLVEEDIVTKVLSFIPDRDSAKSNLAYAD
IVVAGGLGLGSPENFQLVKGLAAVLGAEFGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADLI
VAINTDKNAPIFDFAHIGIVTDAIRLLPALTEAFRARLSPHSRDRIAS

Sequences:

>Translated_368_residues
MSEPTKAPPAAAGRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVDLAAVVIGPPGEAVQHAA
AESFCYGADLAYIVADEVLTDYRNEAYTKALTDTVNTFKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGS
LAATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPERVEKPATRIIEHKLGLVEEDIVTKVLSFIPDRDSAKSNLAYAD
IVVAGGLGLGSPENFQLVKGLAAVLGAEFGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADLI
VAINTDKNAPIFDFAHIGIVTDAIRLLPALTEAFRARLSPHSRDRIAS
>Mature_367_residues
SEPTKAPPAAAGRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKLKVDLAAVVIGPPGEAVQHAAA
ESFCYGADLAYIVADEVLTDYRNEAYTKALTDTVNTFKPEILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGSL
AATRPTFGGSLLCTIYTLNYRPQMATVRPRVMPMPERVEKPATRIIEHKLGLVEEDIVTKVLSFIPDRDSAKSNLAYADI
VVAGGLGLGSPENFQLVKGLAAVLGAEFGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYIAAGISGAIQHRVGVEGADLIV
AINTDKNAPIFDFAHIGIVTDAIRLLPALTEAFRARLSPHSRDRIAS

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family [H]

Homologues:

Organism=Homo sapiens, GI189181759, Length=291, Percent_Identity=32.9896907216495, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4503607, Length=335, Percent_Identity=30.1492537313433, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1787990, Length=271, Percent_Identity=33.9483394833948, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786226, Length=127, Percent_Identity=47.244094488189, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI87082157, Length=305, Percent_Identity=26.8852459016393, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17506929, Length=330, Percent_Identity=32.4242424242424, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325261, Length=333, Percent_Identity=30.6306306306306, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17136898, Length=325, Percent_Identity=31.3846153846154, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24652801, Length=325, Percent_Identity=31.3846153846154, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha [H]

EC number: NA

Molecular weight: Translated: 39463; Mature: 39332

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPTKAPPAAAGRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCHHHHHHH
KVDLAAVVIGPPGEAVQHAAAESFCYGADLAYIVADEVLTDYRNEAYTKALTDTVNTFKP
HEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGSLAATRPTFGGSLLCTIYTLN
CEEEEECHHHCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEEEC
YRPQMATVRPRVMPMPERVEKPATRIIEHKLGLVEEDIVTKVLSFIPDRDSAKSNLAYAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
IVVAGGLGLGSPENFQLVKGLAAVLGAEFGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYI
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHCCCCCCCCCEEEE
AAGISGAIQHRVGVEGADLIVAINTDKNAPIFDFAHIGIVTDAIRLLPALTEAFRARLSP
EECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HSRDRIAS
CCCCCCCC
>Mature Secondary Structure 
SEPTKAPPAAAGRAATKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADKL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEECCCHHHHHHH
KVDLAAVVIGPPGEAVQHAAAESFCYGADLAYIVADEVLTDYRNEAYTKALTDTVNTFKP
HEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EILLLGATTLGRDLAGSVATTLLTGLTADCTELDVDADGSLAATRPTFGGSLLCTIYTLN
CEEEEECHHHCHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEEEC
YRPQMATVRPRVMPMPERVEKPATRIIEHKLGLVEEDIVTKVLSFIPDRDSAKSNLAYAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
IVVAGGLGLGSPENFQLVKGLAAVLGAEFGCSRPLVQKGWVTSDRQIGQTGKTIRPKLYI
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHCCCCCCCCCEEEE
AAGISGAIQHRVGVEGADLIVAINTDKNAPIFDFAHIGIVTDAIRLLPALTEAFRARLSP
EECCCHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HSRDRIAS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850088 [H]