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The map label for this gene is livK [C]

Identifier: 146342227

GI number: 146342227

Start: 5585218

End: 5587572

Strand: Direct

Name: livK [C]

Synonym: BRADO5379

Alternate gene names: 146342227

Gene position: 5585218-5587572 (Clockwise)

Preceding gene: 146342226

Following gene: 146342229

Centisome position: 74.9

GC content: 63.91

Gene sequence:

>2355_bases
ATGATGCAGGGCATCGACACTGCGATTCGTGCCGACGTGACGGCCCCGTGCAGTTCAGGTTTGCCGCGCCCGGCACGGCC
GGTGAGCCGTGCGGAGATGACCGGAAAGGCAGCCTGTCTCGCGCTCGCATTCGCAATTCCGCTGCAGGTCGCCCAGCCCC
GTCCAGCCGCAGCCGCCGGTAATGAAATCCGCATCGGCAACACCATGCCCTATAGCGGCCCGGCGCTCGCCTACGGCGTC
ATCGGCAAGGCGATCACGGCCTATTTCAACAAGGTCAACGCCGAAGGCGGCATCGACGGCCGCCGCATCAGTTTCATTTC
TTATGACGACGGCTACAATCCGCAGAAGACGGTCGAGATGACTCGCAAGCTCGTCGAGGAGGACAATGTCCTGCTGATGT
TTGCCAGCCTGGGCACCGCCACGAGCCTCGCCGTCCGTCCCTATCTGAACGCCAACAAGATTCCGCAGCTCTTCGTCGCT
TCCGGCTCCTCGCAATGGGATCAGTCGCAGGACTTTCCCTGGACCATGGGCTTTCAGCCGAGCTACCAGACCGAGGCGCA
CATCTTCGCGCAATATCTGCTCGAGACGCATTCACGCGGCAAGATCGCGATCCTCTATCAAGATGATGATTTCGGCAAGG
ACTACATCAAGGGCCTGAAGGATGCTCTCGGCGGCAAGATTCCGGTGGTCGCCGAGGCCGGCTACAAGGTCACCGATGCA
AACGTCAACCAGCAGATCGCGACCTTGAAGGCCTCGGGCGCCGACATCCTGCTCGACGTCACCACCCCGAAATTCGCGAT
CATGGCAATCCGGCGAGTCGCCGAGATCGGCTGGCATCCGGACCATCTCCTCACCTCGGTGTCGGATTCGACCTCCGCGG
TGATGCTGGCCGCCGGCGCGCAGAATTCGGAAGGCATCCTGTCCGCCAACTACTTCACCGATGAGGACGACACGGCGACG
ACCGACGATCCGGCCCACCGCGAATGGGCGTCGTTCATGGACCGCTACCTGCCTCAGGTCTCGCGCAGCAACGGACTTGC
GATCTACGGCTATATCGTCGCCGAGACGATGGTGGCCGTGCTGAAGCAATGCGGCGACGATCTGTCGCGCGAGAACATCA
TCAGGCAGGCATCGTCCCTCAAGGGCCTCCAGGTGCCGATGCTGCTGCCGGGCATCACGATCAACACCAGCGTCCACGAT
CACGCGCCGCTGGAGCAGATGCAGATGATGCAGTTCATCGGCGGCAAATGGCAGCGCTTCGGTCCGGTCCGCAGCGGCAT
CGACCCAGGTACCGTCAGCGAGTCCTTCAAGACGATCTTCCGCTACGGCACCGCGAAGAAGGATCTCGCCAATCAGTTGA
ACGCGAACACCGTGACGCTGATGACCGGATCGTTCGGCAGCACCTATGCCAACATGGGCGCGGATCTCGCTTCGGTCCTC
GACAAGGGCGCCGAACTGCGGGTCCTGCCGGTGATCGGTCGCGGCTCGGTGCAGTCGGTGGCCGACATCCTGCTCCTGCG
CGGCGTCGATGCGGGCGTCATCCGCAAGGACACCCTCGCCTTCCTCGAACGGAAGGACTTCGCCAACAACGTCCGCGAGC
AGCTGGTCTATGTCGCCAAGCTGTTCAACGAGGAAATGCACGTGGTGGCGCCGAGATCGATCACCAGCTTGAGCGAGCTC
GACGGCAAGACGATCGCCGTCGACCTTCCCGATGGCGGCACCTTCGTCACCTCGATCAACGTGTTCGAGCGTCTGGGGAT
CAGGCCGCATCTGCTCTACATCGAGCCGCGGCTCGCGGTCGACATGCTGCGCCGGGGCGACATCGACGCGATTATCGCCG
TCGAGGGCAAGCCGCTGCAATGGCTGAGCCAGATCAGGGATCCGAACCTGCATCTCGTGCCGGTCGAGTACGACAAGGCG
CTCCGCGAGGACTATCTGCCGGCGCAGCTGTCAGCCGAGGACTATCCGAACCTCGTCAGCGCCGGCACCCCGGTGAACAC
GATCGCCGCCGAGGCGCTGCTGGCGTCGTATAATTGGCAGGCCGGCAGCGATCGCCACCGCCGGCTGTCGCTGCTGGTCG
AAGCGCTGTTCAACAACCTTCAGACGCTGCAGCGGCCGCCGTTTCATCCGAAATGGCTGGAGGTCGCGCCGCTGGCGCCG
ATCGCCGGCTGGACCCGGTTCAAGACGGCGCAGGACTGGCTCGATCGCAATATGCCGGCAGCCCAGGTGGTGGGCGCCAA
TGCCCAGGCCAGCGACCTCCCGCCGAACGCGGCGAACGACCCGAAACTGTTTCGCGAGTTCCTGGAGTGGCGGGCCAACC
GCCAGAAGCGGCCGGCCGCCCGGCCGCCGCAATGA

Upstream 100 bases:

>100_bases
ACGTCAGATCTCAGCTTCTCAGACGCGATCCGGACCGCCTGGCAACAAGGGCGGCAGCGATCCCCTGCGCTCAAAAGCCG
GACCATCGACGGGAGGATTC

Downstream 100 bases:

>100_bases
TCGATGCAAAGCGTCAGGCCGCTGCCCTGGTGATCCTGACCTGCACGTCGCAGGGACGCGCGAGATAAGGCGCGGAGGTG
ACGAGCACATCGGCGCCAGC

Product: putative ABC/TRAP transporter periplasmic binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 784; Mature: 784

Protein sequence:

>784_residues
MMQGIDTAIRADVTAPCSSGLPRPARPVSRAEMTGKAACLALAFAIPLQVAQPRPAAAAGNEIRIGNTMPYSGPALAYGV
IGKAITAYFNKVNAEGGIDGRRISFISYDDGYNPQKTVEMTRKLVEEDNVLLMFASLGTATSLAVRPYLNANKIPQLFVA
SGSSQWDQSQDFPWTMGFQPSYQTEAHIFAQYLLETHSRGKIAILYQDDDFGKDYIKGLKDALGGKIPVVAEAGYKVTDA
NVNQQIATLKASGADILLDVTTPKFAIMAIRRVAEIGWHPDHLLTSVSDSTSAVMLAAGAQNSEGILSANYFTDEDDTAT
TDDPAHREWASFMDRYLPQVSRSNGLAIYGYIVAETMVAVLKQCGDDLSRENIIRQASSLKGLQVPMLLPGITINTSVHD
HAPLEQMQMMQFIGGKWQRFGPVRSGIDPGTVSESFKTIFRYGTAKKDLANQLNANTVTLMTGSFGSTYANMGADLASVL
DKGAELRVLPVIGRGSVQSVADILLLRGVDAGVIRKDTLAFLERKDFANNVREQLVYVAKLFNEEMHVVAPRSITSLSEL
DGKTIAVDLPDGGTFVTSINVFERLGIRPHLLYIEPRLAVDMLRRGDIDAIIAVEGKPLQWLSQIRDPNLHLVPVEYDKA
LREDYLPAQLSAEDYPNLVSAGTPVNTIAAEALLASYNWQAGSDRHRRLSLLVEALFNNLQTLQRPPFHPKWLEVAPLAP
IAGWTRFKTAQDWLDRNMPAAQVVGANAQASDLPPNAANDPKLFREFLEWRANRQKRPAARPPQ

Sequences:

>Translated_784_residues
MMQGIDTAIRADVTAPCSSGLPRPARPVSRAEMTGKAACLALAFAIPLQVAQPRPAAAAGNEIRIGNTMPYSGPALAYGV
IGKAITAYFNKVNAEGGIDGRRISFISYDDGYNPQKTVEMTRKLVEEDNVLLMFASLGTATSLAVRPYLNANKIPQLFVA
SGSSQWDQSQDFPWTMGFQPSYQTEAHIFAQYLLETHSRGKIAILYQDDDFGKDYIKGLKDALGGKIPVVAEAGYKVTDA
NVNQQIATLKASGADILLDVTTPKFAIMAIRRVAEIGWHPDHLLTSVSDSTSAVMLAAGAQNSEGILSANYFTDEDDTAT
TDDPAHREWASFMDRYLPQVSRSNGLAIYGYIVAETMVAVLKQCGDDLSRENIIRQASSLKGLQVPMLLPGITINTSVHD
HAPLEQMQMMQFIGGKWQRFGPVRSGIDPGTVSESFKTIFRYGTAKKDLANQLNANTVTLMTGSFGSTYANMGADLASVL
DKGAELRVLPVIGRGSVQSVADILLLRGVDAGVIRKDTLAFLERKDFANNVREQLVYVAKLFNEEMHVVAPRSITSLSEL
DGKTIAVDLPDGGTFVTSINVFERLGIRPHLLYIEPRLAVDMLRRGDIDAIIAVEGKPLQWLSQIRDPNLHLVPVEYDKA
LREDYLPAQLSAEDYPNLVSAGTPVNTIAAEALLASYNWQAGSDRHRRLSLLVEALFNNLQTLQRPPFHPKWLEVAPLAP
IAGWTRFKTAQDWLDRNMPAAQVVGANAQASDLPPNAANDPKLFREFLEWRANRQKRPAARPPQ
>Mature_784_residues
MMQGIDTAIRADVTAPCSSGLPRPARPVSRAEMTGKAACLALAFAIPLQVAQPRPAAAAGNEIRIGNTMPYSGPALAYGV
IGKAITAYFNKVNAEGGIDGRRISFISYDDGYNPQKTVEMTRKLVEEDNVLLMFASLGTATSLAVRPYLNANKIPQLFVA
SGSSQWDQSQDFPWTMGFQPSYQTEAHIFAQYLLETHSRGKIAILYQDDDFGKDYIKGLKDALGGKIPVVAEAGYKVTDA
NVNQQIATLKASGADILLDVTTPKFAIMAIRRVAEIGWHPDHLLTSVSDSTSAVMLAAGAQNSEGILSANYFTDEDDTAT
TDDPAHREWASFMDRYLPQVSRSNGLAIYGYIVAETMVAVLKQCGDDLSRENIIRQASSLKGLQVPMLLPGITINTSVHD
HAPLEQMQMMQFIGGKWQRFGPVRSGIDPGTVSESFKTIFRYGTAKKDLANQLNANTVTLMTGSFGSTYANMGADLASVL
DKGAELRVLPVIGRGSVQSVADILLLRGVDAGVIRKDTLAFLERKDFANNVREQLVYVAKLFNEEMHVVAPRSITSLSEL
DGKTIAVDLPDGGTFVTSINVFERLGIRPHLLYIEPRLAVDMLRRGDIDAIIAVEGKPLQWLSQIRDPNLHLVPVEYDKA
LREDYLPAQLSAEDYPNLVSAGTPVNTIAAEALLASYNWQAGSDRHRRLSLLVEALFNNLQTLQRPPFHPKWLEVAPLAP
IAGWTRFKTAQDWLDRNMPAAQVVGANAQASDLPPNAANDPKLFREFLEWRANRQKRPAARPPQ

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 85780; Mature: 85780

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQGIDTAIRADVTAPCSSGLPRPARPVSRAEMTGKAACLALAFAIPLQVAQPRPAAAAG
CCCCCCHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NEIRIGNTMPYSGPALAYGVIGKAITAYFNKVNAEGGIDGRRISFISYDDGYNPQKTVEM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHH
TRKLVEEDNVLLMFASLGTATSLAVRPYLNANKIPQLFVASGSSQWDQSQDFPWTMGFQP
HHHHHCCCCEEEEEECCCCCHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCC
SYQTEAHIFAQYLLETHSRGKIAILYQDDDFGKDYIKGLKDALGGKIPVVAEAGYKVTDA
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCEEECC
NVNQQIATLKASGADILLDVTTPKFAIMAIRRVAEIGWHPDHLLTSVSDSTSAVMLAAGA
CCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECC
QNSEGILSANYFTDEDDTATTDDPAHREWASFMDRYLPQVSRSNGLAIYGYIVAETMVAV
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
LKQCGDDLSRENIIRQASSLKGLQVPMLLPGITINTSVHDHAPLEQMQMMQFIGGKWQRF
HHHHCCHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHHHHHHHHHCCCHHHC
GPVRSGIDPGTVSESFKTIFRYGTAKKDLANQLNANTVTLMTGSFGSTYANMGADLASVL
CCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCHHHHHCHHHHHHH
DKGAELRVLPVIGRGSVQSVADILLLRGVDAGVIRKDTLAFLERKDFANNVREQLVYVAK
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFNEEMHVVAPRSITSLSELDGKTIAVDLPDGGTFVTSINVFERLGIRPHLLYIEPRLAV
HHCCCEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHCCCCEEEEEECCHHHH
DMLRRGDIDAIIAVEGKPLQWLSQIRDPNLHLVPVEYDKALREDYLPAQLSAEDYPNLVS
HHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCHHC
AGTPVNTIAAEALLASYNWQAGSDRHRRLSLLVEALFNNLQTLQRPPFHPKWLEVAPLAP
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
IAGWTRFKTAQDWLDRNMPAAQVVGANAQASDLPPNAANDPKLFREFLEWRANRQKRPAA
CCCCHHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RPPQ
CCCC
>Mature Secondary Structure
MMQGIDTAIRADVTAPCSSGLPRPARPVSRAEMTGKAACLALAFAIPLQVAQPRPAAAAG
CCCCCCHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NEIRIGNTMPYSGPALAYGVIGKAITAYFNKVNAEGGIDGRRISFISYDDGYNPQKTVEM
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHH
TRKLVEEDNVLLMFASLGTATSLAVRPYLNANKIPQLFVASGSSQWDQSQDFPWTMGFQP
HHHHHCCCCEEEEEECCCCCHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCCEEECCCC
SYQTEAHIFAQYLLETHSRGKIAILYQDDDFGKDYIKGLKDALGGKIPVVAEAGYKVTDA
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCEEECC
NVNQQIATLKASGADILLDVTTPKFAIMAIRRVAEIGWHPDHLLTSVSDSTSAVMLAAGA
CCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEECC
QNSEGILSANYFTDEDDTATTDDPAHREWASFMDRYLPQVSRSNGLAIYGYIVAETMVAV
CCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
LKQCGDDLSRENIIRQASSLKGLQVPMLLPGITINTSVHDHAPLEQMQMMQFIGGKWQRF
HHHHCCHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCHHHHHHHHHHCCCHHHC
GPVRSGIDPGTVSESFKTIFRYGTAKKDLANQLNANTVTLMTGSFGSTYANMGADLASVL
CCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCHHHHHCHHHHHHH
DKGAELRVLPVIGRGSVQSVADILLLRGVDAGVIRKDTLAFLERKDFANNVREQLVYVAK
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFNEEMHVVAPRSITSLSELDGKTIAVDLPDGGTFVTSINVFERLGIRPHLLYIEPRLAV
HHCCCEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHCCCCEEEEEECCHHHH
DMLRRGDIDAIIAVEGKPLQWLSQIRDPNLHLVPVEYDKALREDYLPAQLSAEDYPNLVS
HHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCHHC
AGTPVNTIAAEALLASYNWQAGSDRHRRLSLLVEALFNNLQTLQRPPFHPKWLEVAPLAP
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
IAGWTRFKTAQDWLDRNMPAAQVVGANAQASDLPPNAANDPKLFREFLEWRANRQKRPAA
CCCCHHHHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
RPPQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA