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The map label for this gene is wcaJ [H]

Identifier: 146341731

GI number: 146341731

Start: 5041771

End: 5043246

Strand: Direct

Name: wcaJ [H]

Synonym: BRADO4836

Alternate gene names: 146341731

Gene position: 5041771-5043246 (Clockwise)

Preceding gene: 146341728

Following gene: 146341734

Centisome position: 67.61

GC content: 64.43

Gene sequence:

>1476_bases
ATGCATTACGTGGATCAGCCCGAAACTCTCGACCGGAACGGCATGCCTGGGGCCGTATTGGCGGACCGGCAAATTGAATG
TCGGAAATGGCCCATCCATTATGGCGCGGTGGAGCCGTTTGCGATCACGGCGGACATCGCGACGATCGCCGTGACCAGCG
TGCTGTCTGGATATCTCTATCACTTTCAGGACGCGGCCGGCGCGAACGACGTCAGCAAGTCGGTCGGGCTTGCGATCCTC
GTCTCGGCCCTGTTCGTCTCGCTCATGAAGATCCGCGGCATGTATCGGCCGGCGGAGCTCCTGATCCTGTCGCGACAGAT
CCGCGCGACGTGCCTGACCTGGATCACCGCCTTCCTGGTGCTTGCCGGCGCGCTGTTCGCGCTCAAGATCGGCGGCGAGT
TCTCGCGCGGCACCAGCATCGTCTTCGCGATGCTGGGACTGACGGCGCTGGTGCTGAACCGGCGGATGACCGAATATCTG
CTTGCGAAAGGCCTCGCTGAGAAACGCTTTTCAGGACGCAACATCGTCCTGATCACCGACGAGGCGAGCCAGGACAATCC
AGGTCTCAGCCAGGCGCTCGTCGACACCGGCTTTCGCGTCAGGCGGCACTTCCTGCTTCCGGATCCCGGCGCAGCCCCCG
ACCAGCGACAGCACCTGGTGGCCAGCGTGATCGATTATGTGCGCGGCTCGGACATCGAGGAGATTGTGGTCGGCGCCAGC
CCCGATCCCTGGTCCGAACTGCGGACGCTGGGAGCAGACCTCAGGGTTCTGCCCTTCCCCGTAAGCTTCGTGCCCGTCGG
AGCGGCCGCGGAGCTGTTCAATCAGCCGCGTCACGATCTCGGCAGCACCGTCTGCGTCGAGCTGCATCGCGGCCTGATGA
GCCCGCTGGAGCACGCGACCAAGCGTCTCATGGACCTAGCCCTGGCGGGCACCGCTCTGCTGCTGCTGTCGCCCTTGTTG
CTGCTGGTGGCCATCGCCATCAAGCTCGATTCGGCGGGGCCGGTCTTTTTCCGGCAGCAGCGCTGCGGCTTCAACGGCAG
GTCGTTCCAGATCTACAAGTTTCGGACCATGTCGGTGCTCGAGGACGGCCCTACGATCGTCCAGGCGAAGGCCGGGGACA
GCCGGTTCACGCGCCTGGGCGCGTGGCTGCGCCGCACCAGCATCGATGAACTGCCCCAGCTGCTCAATGTCATGAACGGC
AGCATGTCTCTGGTCGGTCCTCGCCCGCACGCCCTGGCGCACGACAACGAATTCGACAAGCTGGTGCGCAACTACGCCTT
CCGCCGCCGTGTAAAGCCGGGCCTGACGGGCTGGGCGCAGGTGCATGGCTGCCGCGGACCGACGCCGACGCGCGCCTCGA
TCGAGCAGCGCGTCGAATTCGACCTCTGGTACATCGACAATTGGAGTCTGTCGCTCGACATCGCGATTTTGCTCAGGACG
CCGATCGAGCTGATCCGAGGCGACAACGCCTATTAG

Upstream 100 bases:

>100_bases
ATTTAAACAACCGGCGATTTTAATTGTTCCAATTAAATGATGAATAATATACTAAGGCGCAAGCCAGCTATTTTAACCGC
GTTTCGAACATCGAGATCCC

Downstream 100 bases:

>100_bases
GCAGCCAAGCTGCCTGCGATCGCATCGCCGGAACATGTCGCGGTGACAAGGCTGGGAAATGCGCGCTGCCTGGTCGGAAA
CGTCAGGTGCCCGGATGCAA

Product: putative sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MHYVDQPETLDRNGMPGAVLADRQIECRKWPIHYGAVEPFAITADIATIAVTSVLSGYLYHFQDAAGANDVSKSVGLAIL
VSALFVSLMKIRGMYRPAELLILSRQIRATCLTWITAFLVLAGALFALKIGGEFSRGTSIVFAMLGLTALVLNRRMTEYL
LAKGLAEKRFSGRNIVLITDEASQDNPGLSQALVDTGFRVRRHFLLPDPGAAPDQRQHLVASVIDYVRGSDIEEIVVGAS
PDPWSELRTLGADLRVLPFPVSFVPVGAAAELFNQPRHDLGSTVCVELHRGLMSPLEHATKRLMDLALAGTALLLLSPLL
LLVAIAIKLDSAGPVFFRQQRCGFNGRSFQIYKFRTMSVLEDGPTIVQAKAGDSRFTRLGAWLRRTSIDELPQLLNVMNG
SMSLVGPRPHALAHDNEFDKLVRNYAFRRRVKPGLTGWAQVHGCRGPTPTRASIEQRVEFDLWYIDNWSLSLDIAILLRT
PIELIRGDNAY

Sequences:

>Translated_491_residues
MHYVDQPETLDRNGMPGAVLADRQIECRKWPIHYGAVEPFAITADIATIAVTSVLSGYLYHFQDAAGANDVSKSVGLAIL
VSALFVSLMKIRGMYRPAELLILSRQIRATCLTWITAFLVLAGALFALKIGGEFSRGTSIVFAMLGLTALVLNRRMTEYL
LAKGLAEKRFSGRNIVLITDEASQDNPGLSQALVDTGFRVRRHFLLPDPGAAPDQRQHLVASVIDYVRGSDIEEIVVGAS
PDPWSELRTLGADLRVLPFPVSFVPVGAAAELFNQPRHDLGSTVCVELHRGLMSPLEHATKRLMDLALAGTALLLLSPLL
LLVAIAIKLDSAGPVFFRQQRCGFNGRSFQIYKFRTMSVLEDGPTIVQAKAGDSRFTRLGAWLRRTSIDELPQLLNVMNG
SMSLVGPRPHALAHDNEFDKLVRNYAFRRRVKPGLTGWAQVHGCRGPTPTRASIEQRVEFDLWYIDNWSLSLDIAILLRT
PIELIRGDNAY
>Mature_491_residues
MHYVDQPETLDRNGMPGAVLADRQIECRKWPIHYGAVEPFAITADIATIAVTSVLSGYLYHFQDAAGANDVSKSVGLAIL
VSALFVSLMKIRGMYRPAELLILSRQIRATCLTWITAFLVLAGALFALKIGGEFSRGTSIVFAMLGLTALVLNRRMTEYL
LAKGLAEKRFSGRNIVLITDEASQDNPGLSQALVDTGFRVRRHFLLPDPGAAPDQRQHLVASVIDYVRGSDIEEIVVGAS
PDPWSELRTLGADLRVLPFPVSFVPVGAAAELFNQPRHDLGSTVCVELHRGLMSPLEHATKRLMDLALAGTALLLLSPLL
LLVAIAIKLDSAGPVFFRQQRCGFNGRSFQIYKFRTMSVLEDGPTIVQAKAGDSRFTRLGAWLRRTSIDELPQLLNVMNG
SMSLVGPRPHALAHDNEFDKLVRNYAFRRRVKPGLTGWAQVHGCRGPTPTRASIEQRVEFDLWYIDNWSLSLDIAILLRT
PIELIRGDNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=295, Percent_Identity=36.9491525423729, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54195; Mature: 54195

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYVDQPETLDRNGMPGAVLADRQIECRKWPIHYGAVEPFAITADIATIAVTSVLSGYLY
CCCCCCCCCCCCCCCCCCEEECCCCCCEECCEECCCCCCEEEEHHHHHHHHHHHHHHHHH
HFQDAAGANDVSKSVGLAILVSALFVSLMKIRGMYRPAELLILSRQIRATCLTWITAFLV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAGALFALKIGGEFSRGTSIVFAMLGLTALVLNRRMTEYLLAKGLAEKRFSGRNIVLITD
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EASQDNPGLSQALVDTGFRVRRHFLLPDPGAAPDQRQHLVASVIDYVRGSDIEEIVVGAS
CCCCCCCCHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCC
PDPWSELRTLGADLRVLPFPVSFVPVGAAAELFNQPRHDLGSTVCVELHRGLMSPLEHAT
CCCHHHHHHCCCCCEEEECCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRLMDLALAGTALLLLSPLLLLVAIAIKLDSAGPVFFRQQRCGFNGRSFQIYKFRTMSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCEEEEEEEEEHHHH
EDGPTIVQAKAGDSRFTRLGAWLRRTSIDELPQLLNVMNGSMSLVGPRPHALAHDNEFDK
CCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHH
LVRNYAFRRRVKPGLTGWAQVHGCRGPTPTRASIEQRVEFDLWYIDNWSLSLDIAILLRT
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCEEEEEEEEHHC
PIELIRGDNAY
CHHHHCCCCCC
>Mature Secondary Structure
MHYVDQPETLDRNGMPGAVLADRQIECRKWPIHYGAVEPFAITADIATIAVTSVLSGYLY
CCCCCCCCCCCCCCCCCCEEECCCCCCEECCEECCCCCCEEEEHHHHHHHHHHHHHHHHH
HFQDAAGANDVSKSVGLAILVSALFVSLMKIRGMYRPAELLILSRQIRATCLTWITAFLV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAGALFALKIGGEFSRGTSIVFAMLGLTALVLNRRMTEYLLAKGLAEKRFSGRNIVLITD
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
EASQDNPGLSQALVDTGFRVRRHFLLPDPGAAPDQRQHLVASVIDYVRGSDIEEIVVGAS
CCCCCCCCHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEECCC
PDPWSELRTLGADLRVLPFPVSFVPVGAAAELFNQPRHDLGSTVCVELHRGLMSPLEHAT
CCCHHHHHHCCCCCEEEECCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRLMDLALAGTALLLLSPLLLLVAIAIKLDSAGPVFFRQQRCGFNGRSFQIYKFRTMSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCEEEEEEEEEHHHH
EDGPTIVQAKAGDSRFTRLGAWLRRTSIDELPQLLNVMNGSMSLVGPRPHALAHDNEFDK
CCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHH
LVRNYAFRRRVKPGLTGWAQVHGCRGPTPTRASIEQRVEFDLWYIDNWSLSLDIAILLRT
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHEEEEEEEECCCEEEEEEEEHHC
PIELIRGDNAY
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]