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The map label for this gene is ptsI [H]

Identifier: 146341514

GI number: 146341514

Start: 4800047

End: 4801654

Strand: Reverse

Name: ptsI [H]

Synonym: BRADO4611

Alternate gene names: 146341514

Gene position: 4801654-4800047 (Counterclockwise)

Preceding gene: 146341515

Following gene: 146341513

Centisome position: 64.39

GC content: 69.15

Gene sequence:

>1608_bases
ATGGTGGAACGCTGCATCCGAGGTCGCGCAGCGTCGGAAGGTCTGGCTATGGGGCCGGCGGTGATCCGGCGCGCGCTGGA
GGGCGCTAGCCGTGCCAAGGGCTCGATTGCAGAGGAGCATGCCGCGCTCGCCGCCGCGGTCGCGCGCGCGCGTGACGCGG
TGGCCGAGTTGATCGCGAGCCTCCAGGGCGATGCGGCCGAGCTGATCGGCTTCCAGCTCGCGCTGCTCGAGGACGATGCG
CTCACGGAAGCCGCCTTTGCGCAGATCGAGCGCGGCACTGCCGCCGAGGATGCCTGGCGCGCAGCCATGGCCCAGGAGAT
CGCGGGCTACGAATCCGCCGATGACGAATATTTCCGCGCCCGCGCCAGCGACTTGGCCGACATCAGGGATCGCGTGGTGC
GACATCTCTTCGGTCTTGCCGATGATGGCCAGCTTCCCAGTGGCGCCATCATCGTCGCGCGTGACCTCGCGCCATCGGCC
TTCCTCGGCGTCGACTGGTCGAAAGGCGGCGGCATTGCGCTGGGCGAGGGCAGCCCAACCAGCCATGTCGCGATGCTCGC
GCGCGGCCGCGGCGTCCCGATGGTGGTGGGGCTCGGCCGCGGCTGGGAGCGCCTGGCAGGAGAGCTGCTGGTCGACGGCA
CGCGCGCCGAGGTCGTTGTCGATCCTGACCCGGAACGCATCGCCGCCGCTCGTAGAGAGATTGATGAGTTCAGGCGCCGC
AAGGCGGAGGCCATCGCTCGCCAGCACGAGCCGGCGTTGACGCGCGACGGAACGCCGGTGGCGGTGCTGGTCAACATTTC
CGGTCCCAATGACATGGCGATCGCGCCGGCATCTGCCGGCGACGGCGTCGGGCTCGCCCGCACCGAATTCCTGACAGCCA
ATCTGCTCCACGACGAAGAGGCGCAATACCGCGCCTATGCCGAGCTGATGCGCTGGGCCGATGGCCGCCCGGTGACGATC
CGTACGCTCGATGCCGGGGGCGACAAGCCGATCCCTGGCTACACGATCGACGGCGAGAGCAATCCGTTCCTCGGACTGCG
CGGGATCCGCCTGTCGCTGCGCCATCCGGAGATCTTCCGCGTACAGCTGCGGGCGTTGTCGCGGGCCGCCGCGCTCGGGC
CGCTGAAGGTGATGCTGCCGATGGTCACCAATGCCGGCGAGCTGGATGCCGCACGCGGGCATCTGCGTGCTGTGCTGGAC
GACCTCGCGAAGGACAGCCTTGCTTTCGCGGAACCGGCCTTTGGCATCATGATCGAGGTGCCGGCCGCGGCGCTGGCCGC
GCAGGATTTTGCCGCCGACTTCTTCTCGATCGGCACCAATGACCTGACCCAATACGCCACCGCGGCGTCGCGCGACGGCG
CCGAGGTGGCCGACTATGCCGACGTGCTGCATCCGGGCGTCCTCGCGATGATCGCGCATGTCGTGCGGGCGGCCCGGGCA
AGAGGCGTCGACGTTTCGATCTGCGGTGATGCCGCGGGTGACGTCAGCGCCATTCCTGCTCTGCTCAGGGCGGGCGTGCG
CGCGCTCTCGGTCTCGCCGGGTTTGCTGCCGCAGGTAAAAGCCGCGATCCGTGAACTCGATCTGGCCAGGCCTGTGGGGA
GCGCATGA

Upstream 100 bases:

>100_bases
GAAGGGCGAGACCCTCTACGTGCGAGCCAATGGTCCGGATGCGGCGGAGGCCGTGCGCGCGCTTGCCGATCTCGTCAACC
GGAAATTCGACGAGGAGTAG

Downstream 100 bases:

>100_bases
GCGAGCAGGACTCATCGGGCTCCGCAAGCCCGCTCGCCGAATACAAGGCGATCCTGCGCGCGGTCATCGACAGCCGCCCG
TCGGGCACGCGGCAGCGGCT

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MVERCIRGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIASLQGDAAELIGFQLALLEDDA
LTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRARASDLADIRDRVVRHLFGLADDGQLPSGAIIVARDLAPSA
FLGVDWSKGGGIALGEGSPTSHVAMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRR
KAEAIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTANLLHDEEAQYRAYAELMRWADGRPVTI
RTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFRVQLRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLD
DLAKDSLAFAEPAFGIMIEVPAAALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARA
RGVDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVGSA

Sequences:

>Translated_535_residues
MVERCIRGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIASLQGDAAELIGFQLALLEDDA
LTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRARASDLADIRDRVVRHLFGLADDGQLPSGAIIVARDLAPSA
FLGVDWSKGGGIALGEGSPTSHVAMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRR
KAEAIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTANLLHDEEAQYRAYAELMRWADGRPVTI
RTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFRVQLRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLD
DLAKDSLAFAEPAFGIMIEVPAAALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARA
RGVDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVGSA
>Mature_535_residues
MVERCIRGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIASLQGDAAELIGFQLALLEDDA
LTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRARASDLADIRDRVVRHLFGLADDGQLPSGAIIVARDLAPSA
FLGVDWSKGGGIALGEGSPTSHVAMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRR
KAEAIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTANLLHDEEAQYRAYAELMRWADGRPVTI
RTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFRVQLRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLD
DLAKDSLAFAEPAFGIMIEVPAAALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARA
RGVDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVGSA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=430, Percent_Identity=36.7441860465116, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1789193, Length=499, Percent_Identity=34.0681362725451, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788726, Length=513, Percent_Identity=30.6042884990253, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI48994992, Length=467, Percent_Identity=31.4775160599572, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1787994, Length=424, Percent_Identity=28.5377358490566, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510935, Length=150, Percent_Identity=35.3333333333333, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 56153; Mature: 56153

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERCIRGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIAS
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQGDAAELIGFQLALLEDDALTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRA
HCCCHHHHHHHEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
RASDLADIRDRVVRHLFGLADDGQLPSGAIIVARDLAPSAFLGVDWSKGGGIALGEGSPT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECEECCCCCEEECCCCCH
SHVAMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRR
HHHHHHHCCCCCEEEEECCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHH
KAEAIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTANLLHDEE
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCH
AQYRAYAELMRWADGRPVTIRTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFR
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEEEECEEEEECCCHHHH
VQLRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLDDLAKDSLAFAEPAFGIMIEV
HHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PAAALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
RGVDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVGSA
CCCEEEECCCCCCCHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVERCIRGRAASEGLAMGPAVIRRALEGASRAKGSIAEEHAALAAAVARARDAVAELIAS
CCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQGDAAELIGFQLALLEDDALTEAAFAQIERGTAAEDAWRAAMAQEIAGYESADDEYFRA
HCCCHHHHHHHEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
RASDLADIRDRVVRHLFGLADDGQLPSGAIIVARDLAPSAFLGVDWSKGGGIALGEGSPT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECEECCCCCEEECCCCCH
SHVAMLARGRGVPMVVGLGRGWERLAGELLVDGTRAEVVVDPDPERIAAARREIDEFRRR
HHHHHHHCCCCCEEEEECCCCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHH
KAEAIARQHEPALTRDGTPVAVLVNISGPNDMAIAPASAGDGVGLARTEFLTANLLHDEE
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCH
AQYRAYAELMRWADGRPVTIRTLDAGGDKPIPGYTIDGESNPFLGLRGIRLSLRHPEIFR
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCEEEEECEEEEECCCHHHH
VQLRALSRAAALGPLKVMLPMVTNAGELDAARGHLRAVLDDLAKDSLAFAEPAFGIMIEV
HHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PAAALAAQDFAADFFSIGTNDLTQYATAASRDGAEVADYADVLHPGVLAMIAHVVRAARA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHH
RGVDVSICGDAAGDVSAIPALLRAGVRALSVSPGLLPQVKAAIRELDLARPVGSA
CCCEEEECCCCCCCHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]