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The map label for this gene is pleD [H]

Identifier: 146341429

GI number: 146341429

Start: 4720818

End: 4722239

Strand: Reverse

Name: pleD [H]

Synonym: BRADO4520

Alternate gene names: 146341429

Gene position: 4722239-4720818 (Counterclockwise)

Preceding gene: 146341430

Following gene: 146341428

Centisome position: 63.33

GC content: 66.39

Gene sequence:

>1422_bases
GTGTCCGCGCGCATCCTTGTCGTCGATGACGTCCCCGCCAACGTCAAGCTGTTGGAGGCACGGCTGTCGGCCGAATATTT
CGACGTGCTGACCGCCTCGAACGGCGCCGAGGCGCTGCAGATCTGCCAGCGCGCCGAATGCGACATCATCCTGCTCGACG
TGATGATGCCGGACATGGACGGCTTCGAGGTTTGCCGCCGGCTGAAGTCGAACCCGGCGACGCATTTCATTCCCGTCGTG
ATGGTGACGGCGCTCGACAGCCCGTCCGACCGCGTGCGCGGCCTCGAGGCCGGCGCCGACGATTTTCTCACCAAGCCGGT
CTCCGACGTCGTGCTGATCGCGCGCGTGCGCTCGCTGACCCGGCTGAAGATGATGACCGACGAGCTGCGCATGCGCGCCC
TGACCTCGCTGGAGATCGGCGTGCAGGCGCCCGAGCGCAACGCGGTCGCCGATGCAGGAAAGGGCGGGCGGATCCTTCTG
GTCGACGACCGGCCGTCGTCCTATGAGCGGCTCGGACCGATGCTGGCGACCGAGCATTCGGTCGATGTCGAGCCGCATCC
GGCGGAGGCCGTGTTCAACGCGGCCGACGGCAATTACGATCTCGTCATCGTCTCGCTCGACCTCGACGATTTCGACGGCT
TGCGGCTGTGCAGCCAGCTGCGTTCGCTGGAGCGCACGCGTCACGTGCCGATCCTGGCGATCGCGGAAGCCGAGAACAAT
TCCCGGCTGCTGCGCGGCCTGGAGATCGGCGTGAACGACTATCTGTTGCGCCCCGTCGATAAGAACGAGCTCTTGGCGCG
CGCGCGCACCCAGATCCGCAAGCGCCGCTACACCGATCACCTGCGCGACAACGTGCAGAACTCGATCGAGATGGCGATCA
CCGACGCGCTGACAGGCCTGCACAATCGCCGTTACATGGAGACGCATCTGTCGACGCTTGCCGACCAGGCGGCGAGCCGC
GGCAAGCCGCTGGCGCTGATGATGCTCGACCTCGACTACTTCAAGTCGATCAACGACACTTACGGCCATGACGCCGGCGA
CGACGTGCTGCGGGAATTCGCCATGCGGGTGCGCAAGTCGATCCGCGGCATCGATCTCGCCTGCCGCTATGGCGGCGAGG
AGTTCGTGATCGTGATGCCGGAGACCGATTTGCACGTCGCCGGCATGGTCGCCGAGCGCCTGCGCCGCTCGGTCGCCAAT
GAGCCGTTCTCGGTGCACAAGGGCGAGAAGCGCATCGACGTCACGGTCTCGATCGGCATCTCCACGTTGGAGCAGAAGGG
CGAGCCGATCGCCGACGTCATGAAGCGCGCCGACACCGCGCTGTACCGCGCCAAGAACGAAGGCCGCAACCGCGCGGTCG
CCATCGCGCCGGTGCATCAGCCATCCTCCTTCCTGCCGCAGGCGGCCGGTCGGGGCCGATAG

Upstream 100 bases:

>100_bases
CATCCGCGAGGGTGGCTGCGAAGCCTATCTTTCTAAACCGATTTCGGTCGGAAAATTCATAGAAACCGTGCGACGTTTCA
TCGGCTGAGGAGTTTCTGCA

Downstream 100 bases:

>100_bases
AGGGCAGGGCGGCTCCGCTCTCTCCTTCGCTGGCTCAAGTTGCCGCCCGTGCGTCCCTGCGTCTGCCATCGTGCCATTCC
GGGCCTCGTGGGCGGGGCGC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALQICQRAECDIILLDVMMPDMDGFEVCRRLKSNPATHFIPVV
MVTALDSPSDRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRALTSLEIGVQAPERNAVADAGKGGRILL
VDDRPSSYERLGPMLATEHSVDVEPHPAEAVFNAADGNYDLVIVSLDLDDFDGLRLCSQLRSLERTRHVPILAIAEAENN
SRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRRYTDHLRDNVQNSIEMAITDALTGLHNRRYMETHLSTLADQAASR
GKPLALMMLDLDYFKSINDTYGHDAGDDVLREFAMRVRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRSVAN
EPFSVHKGEKRIDVTVSIGISTLEQKGEPIADVMKRADTALYRAKNEGRNRAVAIAPVHQPSSFLPQAAGRGR

Sequences:

>Translated_473_residues
MSARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALQICQRAECDIILLDVMMPDMDGFEVCRRLKSNPATHFIPVV
MVTALDSPSDRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRALTSLEIGVQAPERNAVADAGKGGRILL
VDDRPSSYERLGPMLATEHSVDVEPHPAEAVFNAADGNYDLVIVSLDLDDFDGLRLCSQLRSLERTRHVPILAIAEAENN
SRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRRYTDHLRDNVQNSIEMAITDALTGLHNRRYMETHLSTLADQAASR
GKPLALMMLDLDYFKSINDTYGHDAGDDVLREFAMRVRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRSVAN
EPFSVHKGEKRIDVTVSIGISTLEQKGEPIADVMKRADTALYRAKNEGRNRAVAIAPVHQPSSFLPQAAGRGR
>Mature_472_residues
SARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALQICQRAECDIILLDVMMPDMDGFEVCRRLKSNPATHFIPVVM
VTALDSPSDRVRGLEAGADDFLTKPVSDVVLIARVRSLTRLKMMTDELRMRALTSLEIGVQAPERNAVADAGKGGRILLV
DDRPSSYERLGPMLATEHSVDVEPHPAEAVFNAADGNYDLVIVSLDLDDFDGLRLCSQLRSLERTRHVPILAIAEAENNS
RLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRRYTDHLRDNVQNSIEMAITDALTGLHNRRYMETHLSTLADQAASRG
KPLALMMLDLDYFKSINDTYGHDAGDDVLREFAMRVRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRSVANE
PFSVHKGEKRIDVTVSIGISTLEQKGEPIADVMKRADTALYRAKNEGRNRAVAIAPVHQPSSFLPQAAGRGR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=44.7204968944099, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.9746835443038, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=37.2670807453416, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=37.2093023255814, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.7278106508876, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=37.037037037037, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788381, Length=164, Percent_Identity=37.1951219512195, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=38.0368098159509, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=38.135593220339, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=32.7485380116959, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=38.9830508474576, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=39.6694214876033, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=29.3413173652695, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=36.4406779661017, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=39.2, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=171, Percent_Identity=34.5029239766082, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=40.7407407407407, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=33.6134453781513, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=38.135593220339, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=189, Percent_Identity=28.042328042328, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=31.3559322033898, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=33.9449541284404, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=38.4615384615385, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=173, Percent_Identity=28.3236994219653, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790552, Length=120, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788905, Length=106, Percent_Identity=39.622641509434, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789402, Length=118, Percent_Identity=32.2033898305085, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=30.7086614173228, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=34.1880341880342, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=33.8983050847458, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081921, Length=140, Percent_Identity=30, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52503; Mature: 52372

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALQICQRAECDIILLDVMMPDMD
CCCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GFEVCRRLKSNPATHFIPVVMVTALDSPSDRVRGLEAGADDFLTKPVSDVVLIARVRSLT
HHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRMRALTSLEIGVQAPERNAVADAGKGGRILLVDDRPSSYERLGPMLATEHS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCHHCCCCC
VDVEPHPAEAVFNAADGNYDLVIVSLDLDDFDGLRLCSQLRSLERTRHVPILAIAEAENN
CCCCCCCHHHHEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
SRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRRYTDHLRDNVQNSIEMAITDALTGL
CHHHHHEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRRYMETHLSTLADQAASRGKPLALMMLDLDYFKSINDTYGHDAGDDVLREFAMRVRKS
HCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRSVANEPFSVHKGEKRIDVTVSIGI
HCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEECH
STLEQKGEPIADVMKRADTALYRAKNEGRNRAVAIAPVHQPSSFLPQAAGRGR
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCC
>Mature Secondary Structure 
SARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALQICQRAECDIILLDVMMPDMD
CCEEEEEECCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
GFEVCRRLKSNPATHFIPVVMVTALDSPSDRVRGLEAGADDFLTKPVSDVVLIARVRSLT
HHHHHHHHHCCCCHHHHHHHHEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKMMTDELRMRALTSLEIGVQAPERNAVADAGKGGRILLVDDRPSSYERLGPMLATEHS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHCCHHCCCCC
VDVEPHPAEAVFNAADGNYDLVIVSLDLDDFDGLRLCSQLRSLERTRHVPILAIAEAENN
CCCCCCCHHHHEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCC
SRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRRYTDHLRDNVQNSIEMAITDALTGL
CHHHHHEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNRRYMETHLSTLADQAASRGKPLALMMLDLDYFKSINDTYGHDAGDDVLREFAMRVRKS
HCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IRGIDLACRYGGEEFVIVMPETDLHVAGMVAERLRRSVANEPFSVHKGEKRIDVTVSIGI
HCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEECH
STLEQKGEPIADVMKRADTALYRAKNEGRNRAVAIAPVHQPSSFLPQAAGRGR
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA