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The map label for this gene is 146341402

Identifier: 146341402

GI number: 146341402

Start: 4690174

End: 4691250

Strand: Reverse

Name: 146341402

Synonym: BRADO4492

Alternate gene names: NA

Gene position: 4691250-4690174 (Counterclockwise)

Preceding gene: 146341403

Following gene: 146341401

Centisome position: 62.91

GC content: 69.45

Gene sequence:

>1077_bases
ATGTGGCCCGACCGCCGCTTCCTTGATCTCGTTAACACCGAGCACCCGATCGTGCTCGCGCCAATGGCCGGCGTGATGGA
TGCCGATCTCGTCATCGCGGTCGCCCAAGCCGGCGGCCTCGGCTCGCTGCCCTGTGCGATGCTGTCCGCCGACAAGGTGC
GCGAGCAAGTCAGGATCATCCGCCAGCGCGTGTCGGCGCCGGTCAACACGAATTTCTTCTGCCACACGCCCGTGGAGGCC
GACGCGGCTCGTGAGGCCGGGTGGAAGAGGCGGCTCGGCGCCTATTATGAAGAGTATGGCGTCGATCCCGAGGTGCCGGT
CACCGCGGCCAACCGCGCGCCGTTCGATGCGGCGATGTGCGCCATCGTCGAGGAGCTGAAGCCGGAGATCGTCAGCTTCC
ATTTCGGCCTGCCGGAGCAAGGTCTGCTGGCACGTGTGAAGGCGACCGGTGCGGTGATCATGGCCTCTGCAACGGTCGTG
CGCGAGGCGGTCTGGCTGGAGCGGAACGGTGCCGATGTCATCATCGCGCAAGGCGCCGAGGCCGGCGGTCATCGCGGCAT
GTTCCTCACCGACAAGCTCGCCGAGCAGGTCGGGACGTTGGCGCTGGTGCCGCAGATCGTCGATGCGGTGAAGCTGCCGG
TGATCGCGGCCGGCGGGATCGCCGATGCCCGCGGCATCGCGGCGGCATTCGCGCTCGGCGCCTCCGGCGTGCAGATCGGC
AGCGCGTTTCTGCGCTGCCCCGAGTCCAAGGTTAGCGTCCCGGCGCGCGCCGCGCTCGCGGCAGCCAGCGACGAATCGAC
GGTCATCACCAACGTCATGACCGGCCGGCTGGCCCGCGGCGTCGCCAACCGGGTGATGCGCGAGGTCGGGCCGGTCTCGC
CGGACGCGCCGGCGTTTCCGCATGCGGCAACCGCGCTCGGTCCGCTCAAGGCGGCCGCGGAGAAGCAGGGCAAGGTCGAT
TTCACCAACCTTTGGGCCGGGCAGGCGTTGCCGCTCGGGCGCGAGATACCGGCAACCGAGCTCACGCGGACATTGGCCGA
ACAGGCACTGGCGCGCCTCGGCAAGCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGAGATCGTCGCGCGGATCGTCTCAGAGGCCGAGCAGATCCTGCAAGGCCGGCGAAACTCATCCGTGCCGCTCCGGAACT
GAAAGAGAATGAGGTGAACC

Downstream 100 bases:

>100_bases
CGTCAGGGAGACGTCGAGGGCAGGGGAGCTGCCCAGGGAAAGCCTGGGGCTGCCGGTTGCGGCGCGAAAGCGGGCTCTGC
TATAGGGCGGGTACACTGTG

Product: putative 2-nitropropane dioxygenase

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MWPDRRFLDLVNTEHPIVLAPMAGVMDADLVIAVAQAGGLGSLPCAMLSADKVREQVRIIRQRVSAPVNTNFFCHTPVEA
DAAREAGWKRRLGAYYEEYGVDPEVPVTAANRAPFDAAMCAIVEELKPEIVSFHFGLPEQGLLARVKATGAVIMASATVV
REAVWLERNGADVIIAQGAEAGGHRGMFLTDKLAEQVGTLALVPQIVDAVKLPVIAAGGIADARGIAAAFALGASGVQIG
SAFLRCPESKVSVPARAALAAASDESTVITNVMTGRLARGVANRVMREVGPVSPDAPAFPHAATALGPLKAAAEKQGKVD
FTNLWAGQALPLGREIPATELTRTLAEQALARLGKLAG

Sequences:

>Translated_358_residues
MWPDRRFLDLVNTEHPIVLAPMAGVMDADLVIAVAQAGGLGSLPCAMLSADKVREQVRIIRQRVSAPVNTNFFCHTPVEA
DAAREAGWKRRLGAYYEEYGVDPEVPVTAANRAPFDAAMCAIVEELKPEIVSFHFGLPEQGLLARVKATGAVIMASATVV
REAVWLERNGADVIIAQGAEAGGHRGMFLTDKLAEQVGTLALVPQIVDAVKLPVIAAGGIADARGIAAAFALGASGVQIG
SAFLRCPESKVSVPARAALAAASDESTVITNVMTGRLARGVANRVMREVGPVSPDAPAFPHAATALGPLKAAAEKQGKVD
FTNLWAGQALPLGREIPATELTRTLAEQALARLGKLAG
>Mature_358_residues
MWPDRRFLDLVNTEHPIVLAPMAGVMDADLVIAVAQAGGLGSLPCAMLSADKVREQVRIIRQRVSAPVNTNFFCHTPVEA
DAAREAGWKRRLGAYYEEYGVDPEVPVTAANRAPFDAAMCAIVEELKPEIVSFHFGLPEQGLLARVKATGAVIMASATVV
REAVWLERNGADVIIAQGAEAGGHRGMFLTDKLAEQVGTLALVPQIVDAVKLPVIAAGGIADARGIAAAFALGASGVQIG
SAFLRCPESKVSVPARAALAAASDESTVITNVMTGRLARGVANRVMREVGPVSPDAPAFPHAATALGPLKAAAEKQGKVD
FTNLWAGQALPLGREIPATELTRTLAEQALARLGKLAG

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=326, Percent_Identity=30.6748466257669, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 37458; Mature: 37458

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPDRRFLDLVNTEHPIVLAPMAGVMDADLVIAVAQAGGLGSLPCAMLSADKVREQVRII
CCCCHHHHHHHCCCCCEEEECCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RQRVSAPVNTNFFCHTPVEADAAREAGWKRRLGAYYEEYGVDPEVPVTAANRAPFDAAMC
HHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHH
AIVEELKPEIVSFHFGLPEQGLLARVKATGAVIMASATVVREAVWLERNGADVIIAQGAE
HHHHHHCHHHEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCC
AGGHRGMFLTDKLAEQVGTLALVPQIVDAVKLPVIAAGGIADARGIAAAFALGASGVQIG
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCHHHH
SAFLRCPESKVSVPARAALAAASDESTVITNVMTGRLARGVANRVMREVGPVSPDAPAFP
HHHHHCCCHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HAATALGPLKAAAEKQGKVDFTNLWAGQALPLGREIPATELTRTLAEQALARLGKLAG
CHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWPDRRFLDLVNTEHPIVLAPMAGVMDADLVIAVAQAGGLGSLPCAMLSADKVREQVRII
CCCCHHHHHHHCCCCCEEEECCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RQRVSAPVNTNFFCHTPVEADAAREAGWKRRLGAYYEEYGVDPEVPVTAANRAPFDAAMC
HHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHH
AIVEELKPEIVSFHFGLPEQGLLARVKATGAVIMASATVVREAVWLERNGADVIIAQGAE
HHHHHHCHHHEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCEEEEECCCC
AGGHRGMFLTDKLAEQVGTLALVPQIVDAVKLPVIAAGGIADARGIAAAFALGASGVQIG
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCHHHH
SAFLRCPESKVSVPARAALAAASDESTVITNVMTGRLARGVANRVMREVGPVSPDAPAFP
HHHHHCCCHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HAATALGPLKAAAEKQGKVDFTNLWAGQALPLGREIPATELTRTLAEQALARLGKLAG
CHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA