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The map label for this gene is gatA [H]

Identifier: 146341399

GI number: 146341399

Start: 4687996

End: 4689471

Strand: Reverse

Name: gatA [H]

Synonym: BRADO4489

Alternate gene names: 146341399

Gene position: 4689471-4687996 (Counterclockwise)

Preceding gene: 146341400

Following gene: 146341398

Centisome position: 62.89

GC content: 68.22

Gene sequence:

>1476_bases
ATGACCGATTTGACTTCGCTGACGCTCGCCGAGGCCCGCAAGGGCCTCGCGGACAAGACCTTCACGTCGCTCGAGTTGAC
CGACGCCCATCTCAAGGCGATCGAGGCGGCGCGGGCGCTCAATGCCTTCGTGATGGAGACGCCGGATCAGGCGCGCGCGA
TGGCGCGCGACGCCGATGCCAGGATCGGCAAGGGCGAGGGCGGGCCGCTGGCCGGCATTCCGCTGGGGATCAAGGACCTG
TTCGCGACCAAGGACGTCCGCACCACGGCGTGTTCGAAGATCCTGGGTAATTTCGTGCCGACCTACGAGTCGACCGTAAC
CGCGCAGCTGTGGCGCGACGGCGCGGTCATGCTCGGCAAGCTCAACAATGACGAGTTCGCGATGGGCTCGTCCAACGAGA
CCTCGTGCTTCGGCCCGGTCGGCAACCCCTGGCGCCGCGAGGGCTCCAACACCACGCTGGTGCCGGGCGGCTCGTCCGGT
GGCTCGGCGTCCGCGGTGGCGGCGCTGCTTTGCATGGGCGCGACCGCGACCGATACCGGCGGCTCGATCCGCCAGCCGGC
GGCGTTCACCGCGACCGTCGGCATCAAGCCGACCTATGGCCGCTGCTCACGCTGGGGCATCGTCGCCTTCGCCTCGTCGC
TCGACCAGGCCGGTCCAATCGCGCGCACGACGCGCGATGCCGCGATCCTGCTGCGCTCGATGGCCGGCCACGATCCGAAG
GACACGACCTCGGTCGACATTCCCGTGCCGGACTACGAGGCCGCGATCGGAAAGTCCGTGAAGGGCATGAAGATCGGCAT
CCCCAAGGAGTATCGTCTCGACGGCATGCCGGCCGAGATCGAGAAGCTGTGGAGCGAGGGCGCGGCCTGGTTGAAGGCCG
CCGGCGCCGAACTGGTCGAGGTGTCGCTGCCGCACACGAAGTATGCCCTGCCGGCCTATTACATCGTGGCTCCGGCGGAG
GCGTCGTCGAACCTCGCCCGCTACGACGGCGTCCGCTATGGCCTGCGCGTGCCCGGCAAGTCGATCGGCGAGCTGTACGA
GAACACCCGCGCCGAAGGTTTCGGCGCCGAAGTCCGCCGCCGCGTGATGATCGGCACCTATGTGCTGTCGGCCGGCTATT
ACGACGCCTATTACATCCGGGCGCAGAAGGTCCGCACCCTCATCAAGAGGGACTTCGAGGACTGCTTCGCCAAGGGCGTC
AGCGCGATCCTGACGCCGGCGACGCCGTCGGCCGCGTTCGGCGTCGGCGAGAAGGGCGGCGCCGATCCGGTCGAGATGTA
TCTCAACGACATCTTCACGGTGACCGTGAACATGGCGGGCCTGCCGGGCATCGCGGTGCCCGCCGGCAAGGACAGTCAGG
GTCTGCCGCTCGGACTGCAGCTGATCGGCCGTCCGTTCGACGAGGAGACGCTGTTCTCGCTCGGCGAGGTCATCGAGCAG
GCGGCGGGACGCTTCACGCCGGTCCGGTGGTGGTGA

Upstream 100 bases:

>100_bases
CCAACGCCAACAAGGCAACCTCGAAGAAGAACAAGTTCTTCGCCGACGACGCCGACGCCGACAAGACGCTCTCCCCGTTC
TTCTGCAGCCCGATCAACAA

Downstream 100 bases:

>100_bases
GCATCGACGATTTCAAGACGAGCCTCGCGGGCGATGCGCCCGCGGCGGGGCTCACGCCCGTGTTGACCGGCCTGTGGTGG
CTGGCGAAGGGCGATTGGGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTDLTSLTLAEARKGLADKTFTSLELTDAHLKAIEAARALNAFVMETPDQARAMARDADARIGKGEGGPLAGIPLGIKDL
FATKDVRTTACSKILGNFVPTYESTVTAQLWRDGAVMLGKLNNDEFAMGSSNETSCFGPVGNPWRREGSNTTLVPGGSSG
GSASAVAALLCMGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARTTRDAAILLRSMAGHDPK
DTTSVDIPVPDYEAAIGKSVKGMKIGIPKEYRLDGMPAEIEKLWSEGAAWLKAAGAELVEVSLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVPGKSIGELYENTRAEGFGAEVRRRVMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFEDCFAKGV
SAILTPATPSAAFGVGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGKDSQGLPLGLQLIGRPFDEETLFSLGEVIEQ
AAGRFTPVRWW

Sequences:

>Translated_491_residues
MTDLTSLTLAEARKGLADKTFTSLELTDAHLKAIEAARALNAFVMETPDQARAMARDADARIGKGEGGPLAGIPLGIKDL
FATKDVRTTACSKILGNFVPTYESTVTAQLWRDGAVMLGKLNNDEFAMGSSNETSCFGPVGNPWRREGSNTTLVPGGSSG
GSASAVAALLCMGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARTTRDAAILLRSMAGHDPK
DTTSVDIPVPDYEAAIGKSVKGMKIGIPKEYRLDGMPAEIEKLWSEGAAWLKAAGAELVEVSLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVPGKSIGELYENTRAEGFGAEVRRRVMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFEDCFAKGV
SAILTPATPSAAFGVGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGKDSQGLPLGLQLIGRPFDEETLFSLGEVIEQ
AAGRFTPVRWW
>Mature_490_residues
TDLTSLTLAEARKGLADKTFTSLELTDAHLKAIEAARALNAFVMETPDQARAMARDADARIGKGEGGPLAGIPLGIKDLF
ATKDVRTTACSKILGNFVPTYESTVTAQLWRDGAVMLGKLNNDEFAMGSSNETSCFGPVGNPWRREGSNTTLVPGGSSGG
SASAVAALLCMGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARTTRDAAILLRSMAGHDPKD
TTSVDIPVPDYEAAIGKSVKGMKIGIPKEYRLDGMPAEIEKLWSEGAAWLKAAGAELVEVSLPHTKYALPAYYIVAPAEA
SSNLARYDGVRYGLRVPGKSIGELYENTRAEGFGAEVRRRVMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFEDCFAKGVS
AILTPATPSAAFGVGEKGGADPVEMYLNDIFTVTVNMAGLPGIAVPAGKDSQGLPLGLQLIGRPFDEETLFSLGEVIEQA
AGRFTPVRWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=510, Percent_Identity=36.6666666666667, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=22.1327967806841, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=445, Percent_Identity=35.7303370786517, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17537465, Length=521, Percent_Identity=24.5681381957774, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=323, Percent_Identity=25.6965944272446, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=519, Percent_Identity=23.3140655105973, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=35.4166666666667, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=465, Percent_Identity=26.2365591397849, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648113, Length=453, Percent_Identity=39.9558498896247, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24652985, Length=509, Percent_Identity=24.3614931237721, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922090, Length=509, Percent_Identity=24.3614931237721, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24652981, Length=509, Percent_Identity=24.3614931237721, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24652983, Length=509, Percent_Identity=24.3614931237721, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=24.750499001996, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161078093, Length=520, Percent_Identity=24.6153846153846, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648435, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648437, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52099; Mature: 51968

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTSLTLAEARKGLADKTFTSLELTDAHLKAIEAARALNAFVMETPDQARAMARDADA
CCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
RIGKGEGGPLAGIPLGIKDLFATKDVRTTACSKILGNFVPTYESTVTAQLWRDGAVMLGK
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSCFGPVGNPWRREGSNTTLVPGGSSGGSASAVAALLCMGATATDTG
CCCCCEECCCCCCCCEECCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
GSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARTTRDAAILLRSMAGHDPK
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
DTTSVDIPVPDYEAAIGKSVKGMKIGIPKEYRLDGMPAEIEKLWSEGAAWLKAAGAELVE
CCCEEECCCCCHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKSIGELYENTRAEGFGAEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHH
RVMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFEDCFAKGVSAILTPATPSAAFGVGEKGG
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCCCC
ADPVEMYLNDIFTVTVNMAGLPGIAVPAGKDSQGLPLGLQLIGRPFDEETLFSLGEVIEQ
CCHHHHHHHCEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
AAGRFTPVRWW
HCCCCCCCCCC
>Mature Secondary Structure 
TDLTSLTLAEARKGLADKTFTSLELTDAHLKAIEAARALNAFVMETPDQARAMARDADA
CCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
RIGKGEGGPLAGIPLGIKDLFATKDVRTTACSKILGNFVPTYESTVTAQLWRDGAVMLGK
CCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
LNNDEFAMGSSNETSCFGPVGNPWRREGSNTTLVPGGSSGGSASAVAALLCMGATATDTG
CCCCCEECCCCCCCCEECCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
GSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARTTRDAAILLRSMAGHDPK
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
DTTSVDIPVPDYEAAIGKSVKGMKIGIPKEYRLDGMPAEIEKLWSEGAAWLKAAGAELVE
CCCEEECCCCCHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VSLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGKSIGELYENTRAEGFGAEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHH
RVMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFEDCFAKGVSAILTPATPSAAFGVGEKGG
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCCCC
ADPVEMYLNDIFTVTVNMAGLPGIAVPAGKDSQGLPLGLQLIGRPFDEETLFSLGEVIEQ
CCHHHHHHHCEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
AAGRFTPVRWW
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]