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The map label for this gene is cspE [C]

Identifier: 146340625

GI number: 146340625

Start: 3840135

End: 3840788

Strand: Direct

Name: cspE [C]

Synonym: BRADO3673

Alternate gene names: 146340625

Gene position: 3840135-3840788 (Clockwise)

Preceding gene: 146340624

Following gene: 146340628

Centisome position: 51.5

GC content: 65.75

Gene sequence:

>654_bases
ATGGGGTCGGACGGATTTGAGTCCAAGAAGCTTGGAGTACCGGTCGCTGGTGTAACTACACCAAACCGGCTCGGGGCAGA
CTATCAGGTCGGTGTCGCGCGCGACTCGGCGATCGACGCGCTGACCGGTCTTGGCGAGAACCTCGTCGAAATCAGCGGCG
TCATCAAATGGTTCGACGCCTCGAAGGGATACGGCTTCATCGTTCCCGACAACGGCTGGCCCGACGTGCTGCTGCACGTC
ACGGTGCTGAGACGCGATGGCTACCAGACCGCCTATGAAGGCGCTCGGCTGGTGGTCGAGTGCGTCCAGCGCGCCAAGGG
CTACCAGGCCTTCCGCATCGTCTCGATGGACGAGTCGACCGCGATCCATCCGGCGCAGATGCTGCCGCCGCGCACCCATG
TGACGGTGACGCCGACCAGCGGCCTTGAGCGCGCGCAGGTCAAGTGGTTCAACCGCCTTCGCGGCTTCGGCTTCGTCACC
TGCGGCGAGGGCACGCCGGACATCTTCGTCCACATGGAGACCCTGCGCCGGTTCGGCATGACCGAGCTGCGGCCCGGCCA
ATGGGTGCTGGTCCGCTATGGGCCGGGCTCCAAGGGCATGATGGCGGCCGAGATCCATCCCGAGACCGGCTCACCGGCGC
TGTCCTCGCACTGA

Upstream 100 bases:

>100_bases
CGAGAGATTCGCGTGGCTCGTCGAGGGTATAGACGGTGAGCACGCGCAGGGCGCCGTTGCAGGGGTGGCGGCTCACAATT
CATCTGTGAGGTCCGGGGTT

Downstream 100 bases:

>100_bases
CGGGGATCCCGGAGCGAAACGCAAGCAGAGCCCCGGATCCGGGGCTTTGTCGTTTTCTGGTGCCTTCCTCATGCTGATGC
CCGGCCGTGGGAGGGGCTTT

Product: putative DNA binding cold shock protein, CspA-like

Products: NA

Alternate protein names: Transcriptional Regulator Cold Shock Protein; Cold Shock Protein CspA; Cold Shock-Like Protein CspA; Cold Shock Domain-Containing Protein CspD; Cold Shock-Like Protein CspE

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MGSDGFESKKLGVPVAGVTTPNRLGADYQVGVARDSAIDALTGLGENLVEISGVIKWFDASKGYGFIVPDNGWPDVLLHV
TVLRRDGYQTAYEGARLVVECVQRAKGYQAFRIVSMDESTAIHPAQMLPPRTHVTVTPTSGLERAQVKWFNRLRGFGFVT
CGEGTPDIFVHMETLRRFGMTELRPGQWVLVRYGPGSKGMMAAEIHPETGSPALSSH

Sequences:

>Translated_217_residues
MGSDGFESKKLGVPVAGVTTPNRLGADYQVGVARDSAIDALTGLGENLVEISGVIKWFDASKGYGFIVPDNGWPDVLLHV
TVLRRDGYQTAYEGARLVVECVQRAKGYQAFRIVSMDESTAIHPAQMLPPRTHVTVTPTSGLERAQVKWFNRLRGFGFVT
CGEGTPDIFVHMETLRRFGMTELRPGQWVLVRYGPGSKGMMAAEIHPETGSPALSSH
>Mature_216_residues
GSDGFESKKLGVPVAGVTTPNRLGADYQVGVARDSAIDALTGLGENLVEISGVIKWFDASKGYGFIVPDNGWPDVLLHVT
VLRRDGYQTAYEGARLVVECVQRAKGYQAFRIVSMDESTAIHPAQMLPPRTHVTVTPTSGLERAQVKWFNRLRGFGFVTC
GEGTPDIFVHMETLRRFGMTELRPGQWVLVRYGPGSKGMMAAEIHPETGSPALSSH

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=37.6811594202899, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=38.8059701492537, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=36.7647058823529, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23649; Mature: 23518

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDGFESKKLGVPVAGVTTPNRLGADYQVGVARDSAIDALTGLGENLVEISGVIKWFDA
CCCCCCCCCCCCCEEECCCCCHHCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHEEEEC
SKGYGFIVPDNGWPDVLLHVTVLRRDGYQTAYEGARLVVECVQRAKGYQAFRIVSMDEST
CCCCEEEECCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
AIHPAQMLPPRTHVTVTPTSGLERAQVKWFNRLRGFGFVTCGEGTPDIFVHMETLRRFGM
CCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHHHHCCC
TELRPGQWVLVRYGPGSKGMMAAEIHPETGSPALSSH
CCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GSDGFESKKLGVPVAGVTTPNRLGADYQVGVARDSAIDALTGLGENLVEISGVIKWFDA
CCCCCCCCCCCCEEECCCCCHHCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHEEEEC
SKGYGFIVPDNGWPDVLLHVTVLRRDGYQTAYEGARLVVECVQRAKGYQAFRIVSMDEST
CCCCEEEECCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
AIHPAQMLPPRTHVTVTPTSGLERAQVKWFNRLRGFGFVTCGEGTPDIFVHMETLRRFGM
CCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHHHHCCC
TELRPGQWVLVRYGPGSKGMMAAEIHPETGSPALSSH
CCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA