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The map label for this gene is phbC [H]

Identifier: 146340526

GI number: 146340526

Start: 3760978

End: 3762783

Strand: Reverse

Name: phbC [H]

Synonym: BRADO3568

Alternate gene names: 146340526

Gene position: 3762783-3760978 (Counterclockwise)

Preceding gene: 146340533

Following gene: 146340525

Centisome position: 50.46

GC content: 62.13

Gene sequence:

>1806_bases
ATGACCGACGTCCCGAACAACACCAATCCACAAAAAACCTTCGACGCCGAAGCCTTCGCGACGAATATCGCGAGAGCAAT
GGAGAGCAGCGGCAAGGCCCTCGCCGCCTATCTGAAACCGCGCGAAACCGGCGAGGTTCAGGACCGTCCGCCGACCGAAC
TCACCGAAGTCGTGAAGTCGTTCACGGCAGTCGCCGACTACTGGCTGTCCGACAAGGATCGCGCCAGCGACATCCAAACC
AAGCTCGCCAAGGGCTATCTCGACTTGTGGGGCTCGGCGGCGCGACGGTTGGCCGGCGAGGAGGCGCCGCCGGCGATCTC
GCCGTCGCCGCGCGACAAACGCTTTGCCGATCCTGAGTGGAAGTCGAACCAGTTCTACGATTTCGTCATGCAGGCGTATC
TGCTGACGACCCAGTGGGCGCAAGACCTCGTGCACAATGCCGAAGGCCTCGATCCGCATACGCGCAAGAAGGCCGAGTTC
TACGTCAACCAGATCACCAACGCGCTGGCGCCGTCGAACTTCGTGATGACCAACCCGGAGGTGATGCGGCAGACCGTTGC
GTCGAGCGGCGACAATCTGGTGCGCGGCATGCAGATGCTCGCCGAGGACATCGAGGCCGGCAAGGGCACGCTGAAGATCC
GCCAATCCGATCCGGCCAATCTGGAGGTCGGCGTCAACATGGCGACGACGCCAGGCAAGGTGATCTTCCAGAACGAGATG
ATGCAGCTGATCCAGTACGCGCCGACGACGGAAACCGTGCTGCGGACGCCGCTGTTGATCGTGCCGCCCTGGATCAACAA
GTTCTACATCCTCGACCTCAGGCCGGAAAAATCCTTCATCAAATGGTGCGTCGATCAGGGCCTCACGGTGTTCGTGATCT
CCTGGGTCAATCCCGACAAGAAGCTGGGGACCAAGACCTGGGAAGACTACATGAAGGAAGGTCCGCTCACCGCGATGGAC
GTCATCGAGAAGGTGACGGGCGAGATGAAGGTGCACACGATGGGCTATTGCGTCGGCGGCACCATGCTGGCGACGACGCT
GGCCTGGCTCGCCGACAAGCGCCGCCAGCGCGTGACCTCGGCGACGTTCCTCGCCGCGCAGGTCGACTTCACCCATGCCG
GCGATCTCCTGGTGTTCGTCGACGAGACCCAGATTTCGGCGCTGGAGCGCGACATGCAGGCCTCGGGTGTGCTCGAGGGC
AGCAAGATGGCGATGGCCTTCAACATGCTCAGGTCGAACGATCTGATCTGGTCCTATGTCGTGAACAACTACCTCAAGGG
CCAACCGCCGCAGGCGTTCGACCTGCTGCACTGGAATTCGGACGCCACGCGGATGTCGGCGGCCAATCATTCCTATTATC
TTCGCAACTGCTATCTGGAGAATCGCCTGACCACGGGCACCATGGTGCTGGACAACACGCTGCTCGACCTCTCCAAGGTC
AAGGTGCCCGTCTACAACCTGGCCACCCGCGAGGACCATATCGCGCCGGCGGACTCCGTGCTCTACGGTTCGCAATTCTT
CGGCGGGCCGGTGAAGTATGTGCTGTCGGGCTCCGGACATATCGCCGGTGTGGTCAATCCGCCCTCCTCCGGCAAATACC
AGTACTGGACCAACGACCAGATCCACGACATTTCCCTCAAGGACTGGATGAAGGGCGCTCAGGAGCACAAGGGCTCGTGG
TGGCCGGACTGGCGCGAATGGCTCGGCCAGCTCGATCCGGAACAGGTTCCCGCCCGCAGTGTCGGCAGCGAGGCCTATCC
TCCGATCGAGGATGCCCCGGGCTCCTATGTCCGCGTCCGCGCATAG

Upstream 100 bases:

>100_bases
TTCGCAGCGCAACAGACGGACATCGAATTAACCCCAAACTCTCGGATTGTTGATCTCGCGCGGTGACGAAACGATTCCGA
CGCGATAGTGTGAGCCCGCC

Downstream 100 bases:

>100_bases
CGCCGTCAGCCGCAATTGACTATAACCGCTCCGTCCCGCCGGGACGGGACGGAATCAGTTGAGGGGATCTCGATGACGCG
TGAACTGTTCTGGCTGACGC

Product: Poly-beta-hydroxybutyrate polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MTDVPNNTNPQKTFDAEAFATNIARAMESSGKALAAYLKPRETGEVQDRPPTELTEVVKSFTAVADYWLSDKDRASDIQT
KLAKGYLDLWGSAARRLAGEEAPPAISPSPRDKRFADPEWKSNQFYDFVMQAYLLTTQWAQDLVHNAEGLDPHTRKKAEF
YVNQITNALAPSNFVMTNPEVMRQTVASSGDNLVRGMQMLAEDIEAGKGTLKIRQSDPANLEVGVNMATTPGKVIFQNEM
MQLIQYAPTTETVLRTPLLIVPPWINKFYILDLRPEKSFIKWCVDQGLTVFVISWVNPDKKLGTKTWEDYMKEGPLTAMD
VIEKVTGEMKVHTMGYCVGGTMLATTLAWLADKRRQRVTSATFLAAQVDFTHAGDLLVFVDETQISALERDMQASGVLEG
SKMAMAFNMLRSNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMSAANHSYYLRNCYLENRLTTGTMVLDNTLLDLSKV
KVPVYNLATREDHIAPADSVLYGSQFFGGPVKYVLSGSGHIAGVVNPPSSGKYQYWTNDQIHDISLKDWMKGAQEHKGSW
WPDWREWLGQLDPEQVPARSVGSEAYPPIEDAPGSYVRVRA

Sequences:

>Translated_601_residues
MTDVPNNTNPQKTFDAEAFATNIARAMESSGKALAAYLKPRETGEVQDRPPTELTEVVKSFTAVADYWLSDKDRASDIQT
KLAKGYLDLWGSAARRLAGEEAPPAISPSPRDKRFADPEWKSNQFYDFVMQAYLLTTQWAQDLVHNAEGLDPHTRKKAEF
YVNQITNALAPSNFVMTNPEVMRQTVASSGDNLVRGMQMLAEDIEAGKGTLKIRQSDPANLEVGVNMATTPGKVIFQNEM
MQLIQYAPTTETVLRTPLLIVPPWINKFYILDLRPEKSFIKWCVDQGLTVFVISWVNPDKKLGTKTWEDYMKEGPLTAMD
VIEKVTGEMKVHTMGYCVGGTMLATTLAWLADKRRQRVTSATFLAAQVDFTHAGDLLVFVDETQISALERDMQASGVLEG
SKMAMAFNMLRSNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMSAANHSYYLRNCYLENRLTTGTMVLDNTLLDLSKV
KVPVYNLATREDHIAPADSVLYGSQFFGGPVKYVLSGSGHIAGVVNPPSSGKYQYWTNDQIHDISLKDWMKGAQEHKGSW
WPDWREWLGQLDPEQVPARSVGSEAYPPIEDAPGSYVRVRA
>Mature_600_residues
TDVPNNTNPQKTFDAEAFATNIARAMESSGKALAAYLKPRETGEVQDRPPTELTEVVKSFTAVADYWLSDKDRASDIQTK
LAKGYLDLWGSAARRLAGEEAPPAISPSPRDKRFADPEWKSNQFYDFVMQAYLLTTQWAQDLVHNAEGLDPHTRKKAEFY
VNQITNALAPSNFVMTNPEVMRQTVASSGDNLVRGMQMLAEDIEAGKGTLKIRQSDPANLEVGVNMATTPGKVIFQNEMM
QLIQYAPTTETVLRTPLLIVPPWINKFYILDLRPEKSFIKWCVDQGLTVFVISWVNPDKKLGTKTWEDYMKEGPLTAMDV
IEKVTGEMKVHTMGYCVGGTMLATTLAWLADKRRQRVTSATFLAAQVDFTHAGDLLVFVDETQISALERDMQASGVLEGS
KMAMAFNMLRSNDLIWSYVVNNYLKGQPPQAFDLLHWNSDATRMSAANHSYYLRNCYLENRLTTGTMVLDNTLLDLSKVK
VPVYNLATREDHIAPADSVLYGSQFFGGPVKYVLSGSGHIAGVVNPPSSGKYQYWTNDQIHDISLKDWMKGAQEHKGSWW
PDWREWLGQLDPEQVPARSVGSEAYPPIEDAPGSYVRVRA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 67324; Mature: 67193

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPNNTNPQKTFDAEAFATNIARAMESSGKALAAYLKPRETGEVQDRPPTELTEVVKS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHH
FTAVADYWLSDKDRASDIQTKLAKGYLDLWGSAARRLAGEEAPPAISPSPRDKRFADPEW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
KSNQFYDFVMQAYLLTTQWAQDLVHNAEGLDPHTRKKAEFYVNQITNALAPSNFVMTNPE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHH
VMRQTVASSGDNLVRGMQMLAEDIEAGKGTLKIRQSDPANLEVGVNMATTPGKVIFQNEM
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEHHHH
MQLIQYAPTTETVLRTPLLIVPPWINKFYILDLRPEKSFIKWCVDQGLTVFVISWVNPDK
HHHHHCCCCHHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCH
KLGTKTWEDYMKEGPLTAMDVIEKVTGEMKVHTMGYCVGGTMLATTLAWLADKRRQRVTS
HHCCHHHHHHHHCCCCHHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ATFLAAQVDFTHAGDLLVFVDETQISALERDMQASGVLEGSKMAMAFNMLRSNDLIWSYV
HHEEEEEECEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
VNNYLKGQPPQAFDLLHWNSDATRMSAANHSYYLRNCYLENRLTTGTMVLDNTLLDLSKV
HHHHHCCCCCCCEEEEEECCCHHHHHHCCCCEEEHHEEECCCCCCCEEEECHHHHHHHHC
KVPVYNLATREDHIAPADSVLYGSQFFGGPVKYVLSGSGHIAGVVNPPSSGKYQYWTNDQ
CCCCEEECCCCCCCCCCHHHEECCHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCC
IHDISLKDWMKGAQEHKGSWWPDWREWLGQLDPEQVPARSVGSEAYPPIEDAPGSYVRVR
EEECCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHCCHHHCCCCCCCCCCCCCCCEEEEE
A
C
>Mature Secondary Structure 
TDVPNNTNPQKTFDAEAFATNIARAMESSGKALAAYLKPRETGEVQDRPPTELTEVVKS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHH
FTAVADYWLSDKDRASDIQTKLAKGYLDLWGSAARRLAGEEAPPAISPSPRDKRFADPEW
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
KSNQFYDFVMQAYLLTTQWAQDLVHNAEGLDPHTRKKAEFYVNQITNALAPSNFVMTNPE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHH
VMRQTVASSGDNLVRGMQMLAEDIEAGKGTLKIRQSDPANLEVGVNMATTPGKVIFQNEM
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEHHHH
MQLIQYAPTTETVLRTPLLIVPPWINKFYILDLRPEKSFIKWCVDQGLTVFVISWVNPDK
HHHHHCCCCHHHHHHCCEEEECCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCH
KLGTKTWEDYMKEGPLTAMDVIEKVTGEMKVHTMGYCVGGTMLATTLAWLADKRRQRVTS
HHCCHHHHHHHHCCCCHHHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ATFLAAQVDFTHAGDLLVFVDETQISALERDMQASGVLEGSKMAMAFNMLRSNDLIWSYV
HHEEEEEECEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHH
VNNYLKGQPPQAFDLLHWNSDATRMSAANHSYYLRNCYLENRLTTGTMVLDNTLLDLSKV
HHHHHCCCCCCCEEEEEECCCHHHHHHCCCCEEEHHEEECCCCCCCEEEECHHHHHHHHC
KVPVYNLATREDHIAPADSVLYGSQFFGGPVKYVLSGSGHIAGVVNPPSSGKYQYWTNDQ
CCCCEEECCCCCCCCCCHHHEECCHHCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCC
IHDISLKDWMKGAQEHKGSWWPDWREWLGQLDPEQVPARSVGSEAYPPIEDAPGSYVRVR
EEECCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHCCHHHCCCCCCCCCCCCCCCEEEEE
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]