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The map label for this gene is hisJ [C]

Identifier: 146340448

GI number: 146340448

Start: 3670806

End: 3671639

Strand: Reverse

Name: hisJ [C]

Synonym: BRADO3488

Alternate gene names: 146340448

Gene position: 3671639-3670806 (Counterclockwise)

Preceding gene: 146340449

Following gene: 146340441

Centisome position: 49.24

GC content: 65.95

Gene sequence:

>834_bases
TTGCGCTATGCGCTGGCGATCGGCTCAGGCGCCGCGCTCGCGTCCGCGATGACGACGACGCCGGCCTCGGCGCAGGCGGC
CGCCGACAACACGCTCGACCGGGTTCGCGCCAGCAAGGTGCTGCGCATCGCCGTGCTGCCGGGCGAGCTTCCCTATTTCA
ACAAGGACCTCGCCTCCGGCACCTGGTCGGGCTTCTCGATCGAGATGGCCAACGACATCGCCAAGCTGCTCGACGTGAAG
CTCGAATATGTCGAATCGACCTACGGCAATTCGGTGCTCGACCTGCAGGCCGGCAAGATCGATCTCGGCTTCGCGCTCAA
CCCGACGCCGCAGCGCGCGCTGGTGATCGACTTCTGCGGCACGGTGTTCCCGCATCCGTTCGGCGCGATGCTGAAGAAGG
GCATGGAAGCCAAGACCTGGGCCGACATCAACAAGCCGGAGGTGAAGATCGCCGTCGACGTCGGCTCGGCCAACGAGGCG
GTGGCGCGGCGCTTCGCGCCGAACGCGACCATCAAGTCGCTGAAGTCGCGCGACGAGGTGATGCTGGAGATGGCCTCGGG
CCGCGTCGACGTCGTCGTCAACGCCCTGATCCTCGGCCTCACCGCGATCGCCAAGAACCCCAATCTCGGAAGCTACAAGA
TCCTGCAGTCGCCGTCGGTCGCGATCCCCTCCAGCATGGGTGTCCGCCGCGAGGCCGACAAGCGCTGGCGCGACTTCCTC
GCGGTGTGGATCGACTACAACCGCGGCATCGGCCAGATGCGCGAATGGTTCGTCAAGGGCCTGGGGCTTGCCGGCGTCAA
GGCCGAGGATGTGCCGGTCGAAGTGAATATCTAA

Upstream 100 bases:

>100_bases
TGGCGTGCGACGCCGACGCTACGCTCCGCGCCGATTGGTGCAGGAGGATGTTGTGACCACGTCAGACGATCAGACCATTT
CCGAAGACCGCCGCGAGGCC

Downstream 100 bases:

>100_bases
GGCGGCCCCGCTCTCTCCACCGTCATTGCGAGGCGCGTAGCGACGAAGCAATCCAGAGCTCAGGTTGCAGCCCTGGATTG
CTTCGCTTCGCTCGCAATGA

Product: putative signal peptide

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: Carboxycyclohexadienyl Dehydratase; Amino Acid ABC Transporter; Prephrenate Dehydratase; Signal Peptide; Cyclohexadienyl Dehydratase Periplasmic; Soluble Lytic Transglycosylase; Cyclohexadienyl Dehydratase Signal Peptide Protein

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MRYALAIGSGAALASAMTTTPASAQAAADNTLDRVRASKVLRIAVLPGELPYFNKDLASGTWSGFSIEMANDIAKLLDVK
LEYVESTYGNSVLDLQAGKIDLGFALNPTPQRALVIDFCGTVFPHPFGAMLKKGMEAKTWADINKPEVKIAVDVGSANEA
VARRFAPNATIKSLKSRDEVMLEMASGRVDVVVNALILGLTAIAKNPNLGSYKILQSPSVAIPSSMGVRREADKRWRDFL
AVWIDYNRGIGQMREWFVKGLGLAGVKAEDVPVEVNI

Sequences:

>Translated_277_residues
MRYALAIGSGAALASAMTTTPASAQAAADNTLDRVRASKVLRIAVLPGELPYFNKDLASGTWSGFSIEMANDIAKLLDVK
LEYVESTYGNSVLDLQAGKIDLGFALNPTPQRALVIDFCGTVFPHPFGAMLKKGMEAKTWADINKPEVKIAVDVGSANEA
VARRFAPNATIKSLKSRDEVMLEMASGRVDVVVNALILGLTAIAKNPNLGSYKILQSPSVAIPSSMGVRREADKRWRDFL
AVWIDYNRGIGQMREWFVKGLGLAGVKAEDVPVEVNI
>Mature_277_residues
MRYALAIGSGAALASAMTTTPASAQAAADNTLDRVRASKVLRIAVLPGELPYFNKDLASGTWSGFSIEMANDIAKLLDVK
LEYVESTYGNSVLDLQAGKIDLGFALNPTPQRALVIDFCGTVFPHPFGAMLKKGMEAKTWADINKPEVKIAVDVGSANEA
VARRFAPNATIKSLKSRDEVMLEMASGRVDVVVNALILGLTAIAKNPNLGSYKILQSPSVAIPSSMGVRREADKRWRDFL
AVWIDYNRGIGQMREWFVKGLGLAGVKAEDVPVEVNI

Specific function: Component Of The High-Affinity Histidine Permease, A Binding-Protein-Dependent Transport System. The Other Components Are Proteins Hisq, Hism, And Hisp. [C]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29841; Mature: 29841

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYALAIGSGAALASAMTTTPASAQAAADNTLDRVRASKVLRIAVLPGELPYFNKDLASG
CEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHCCC
TWSGFSIEMANDIAKLLDVKLEYVESTYGNSVLDLQAGKIDLGFALNPTPQRALVIDFCG
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCEEEEEHHH
TVFPHPFGAMLKKGMEAKTWADINKPEVKIAVDVGSANEAVARRFAPNATIKSLKSRDEV
HHCCCCHHHHHHCCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHH
MLEMASGRVDVVVNALILGLTAIAKNPNLGSYKILQSPSVAIPSSMGVRREADKRWRDFL
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHE
AVWIDYNRGIGQMREWFVKGLGLAGVKAEDVPVEVNI
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MRYALAIGSGAALASAMTTTPASAQAAADNTLDRVRASKVLRIAVLPGELPYFNKDLASG
CEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHCCC
TWSGFSIEMANDIAKLLDVKLEYVESTYGNSVLDLQAGKIDLGFALNPTPQRALVIDFCG
CCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCEEEEEHHH
TVFPHPFGAMLKKGMEAKTWADINKPEVKIAVDVGSANEAVARRFAPNATIKSLKSRDEV
HHCCCCHHHHHHCCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHH
MLEMASGRVDVVVNALILGLTAIAKNPNLGSYKILQSPSVAIPSSMGVRREADKRWRDFL
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHE
AVWIDYNRGIGQMREWFVKGLGLAGVKAEDVPVEVNI
EEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA